Information of genes involved in ethylene signaling in <i>Morus notabilis</i>.

<p>^a The CDS length was predicted according to the predicted <i>Morus</i> genes.</p><p>^b The number of exon was predicted based on the <i>Morus</i> genomic data.</p><p>Information of genes involved in ethylene signaling in <i>Morus notabilis</i>.</p

Changes in ethylene production, SSC and firmness during fruit development of <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40.

<p>(A) <i>Morus</i> fruit during development. (B) The ethylene production, SSC and firmness. The batch 1 fruits were used for these experiments. Error bars on each column indicate SDs from eight replicates.</p

Gene structure and predicted functional domains of <i>Morus notabilis</i> ethylene signaling genes.

<p>Gene structures were obtained by aligning the cloned cDNA sequences with the <i>M</i>. <i>notabilis</i> genome data and functional domains were predicted in SMART (<a href="http://smart.embl-heidelberg.de/smart/set_mode.cgi?NORMAL=1" target="_blank">http://smart.embl-heidelberg.de/smart/set_mode.cgi?NORMAL=1</a>). Gene structures were displayed by Fancy Gene (<a href="http://bio.ieo.eu/fancygene/" target="_blank">http://bio.ieo.eu/fancygene/</a>)</p

Primers used for qRT-PCR in <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40.

<p>Primers used for qRT-PCR in <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40.</p

Effects of exogenous C₂H₄ and 1-MCP on postharvest fruit of <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40 at 23°C.

<p>The batch 2 fruits were divided into three groups. Two groups were treated with ethylene and 1-MCP, respectively. The third group of fruits was sealed in a similar container of the same volume as a control. Error bars on each column indicate SDs from eight replicates.</p

Primers used for cloning the genes involved in the ethylene signal transduction in <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40.

<p>Primers used for cloning the genes involved in the ethylene signal transduction in <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40.</p

The expression profiles of genes involved in ethylene signal transduction in <i>Morus notabilis</i>.

<p>Expression analysis of genes involved in ethylene signal transduction based on the RPKM (reads per kilobase of exon model per million mapped reads) profile of five tissues (root, bark, bud, flower, and leaf). Cluster 3.0 software was used to normalize the expression level of the ethylene signal-related genes from RNA sequencing data. Sample names are shown above the heat maps. Color scale indicates the degree of expression: green, low expression; red, high expression; grey, no expression.</p

Expression of the genes involved in ethylene biosynthesis and signaling pathway during fruit ripening of <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40.

<p>The batch 2 fruits were used for qRT-PCR. Error bars on each column indicate SDs from three replicates. Significant differences (<i>P</i><0.05) among treatment are marked with different letters above bars.</p

Expression of different components of the ethylene signaling pathway during fruit development of <i>Morus atropurpurea</i> cv. <i>Jialing</i> No.40.

<p>The batch 1 fruits were used for qRT-PCR. Each column height indicates relative mRNA abundance. Error bars on each column indicate SDs from three replicates. Significant differences (<i>P</i><0.05) are marked with different letters above bars.</p

Phylogenetic analysis of MnETRs (A), MnCTR1 (B), MnRTE (C), and MnEILs(D).

<p>The amino acid sequences were analyzed with MUSCLE 3.6 and the phylogenetic tree constructed with MEGA 5.0 using a bootstrap test of phylogeny with minimum evolution test and default parameters. AtETR1 (AAA70047.1), AtERS1 (NP_181626.1), AtETR2 (NP_188956.1), AtERS2 (AAC62209.1), AtEIN4(AAD02485.1), AtCTR1 (AAA32780.1), AtEDR1 (AAG31143.1), AtRTE1(F4ITL6.1), AtRTE2(Q9SD42.1), AtEIN3 (NP_188713.1), AtEIL1 (NP_180273.1), AtEIL2 (NP_197611.1), AtEIL3 (NP_177514.1) and AtEIL4(NP_196574.1) in <i>Arabidopsis</i>; LeETR1 (AAC02213.1), LeETR2 (AAC02214.1), LeETR3 (AAC49124.1), LeETR4 (AAU34076.1), LeETR5 (AAD31397.1), LeETR6 (AAL86614.1), LeCTR1(AAL87456.1), TCTR2 (CAA06334.1), LeCTR3 (AAR89820.1), LeCTR4 (AAR89822.1), LeEIL1 (AAK58857.1), LeEIL2 (AAK58858.1), LeEIL3 (AAK58859.1) and LeEIL4 (BAC99307.1) in <i>Solanum lycopersicum</i>; PmRTE (XP_008220470.1) in <i>Prunus mume</i>; ObRTE (XP_006654719.1) in <i>Oryza brachyantha</i>; CsRTE (XP_006475936.1) in <i>Citrus sinensis</i>; StRTE (XP_006349393.1) in <i>Solanum tuberosum</i>; SiRTE (XP_004961370.1) in <i>Setaria italic</i>; CaRTE (XP_004498900.1) in <i>Cicer arietinum</i>; FaRTE (XP_004291036.1) in <i>Fragaria vesca subsp</i>. <i>Vesca</i>; CsRTE1 (XP_004157357.1) in <i>Cucumis sativus</i>; CsRTE2 (XP_004141314.1) in <i>Cucumis sativus</i>; DcRTE1 (ADW80941.1) in <i>Dianthus caryophyllus</i>.</p