42 research outputs found
Evaluation of cathepsin D immunostaining in colorectal adenocarcinoma
Background and Objectives: Cathepsin D (CD), an estrogen-regulated
lysosomal protease, has been detected in a variety of tissues, Cn
expression has been correlated with the invasive potential of breast
cancer, acting as an autocrine mitogen or as a protease that degrades
the extracellular matrix, The role of CD expression in predicting
prognosis or invasive potential in colorectal carcinomas is mostly
unknown.
Methods: CD immunohistochemical expression was studied in 60 surgical
specimens of colon adenocarcinomas, A three-step avidin biotinylated,
horseradish immuno-peroxidase (ABC-HRP) staining technique was performed
on 4 mu m paraffin-embedded tissue sections with a polyclonal antibody
to CD.
Results: Carcinoma cells showed positive CD immunostaining in 41.6% of
adenocarcinomas (50%, 43.7%, 37.5%, and 25% of Dukes’ Stage A, B, C,
and D, respectively). Nonneoplastic stromal cells demonstrated positive
staining in 68.3% of the adenocarcinoma specimens (37.5%, 62.5%,
91.6%, and 75% of Stage A, B, C, and D, respectively). Patients with
coloreclal carcinomas exhibiting simultaneously negative and positive CD
expression in malignant and stromal cells, respectively, had a worse
5-year overall survival (P < 0.05). The mean 5-year survival of the 16
patients overexpressing CD in nonneoplastic stromal cells (>15% of
stromal cells positive for CD) was significantly worse in comparison
with the rest of the adenocarcinomas (n = 44) (27.6 +/- 4.6 vs. 46 +/-
2.7 months, respectively, P < 0.01).
Conclusions: Expression of CD immunoreactivity by the stromal cells may
be associated with a more invasive phenotype. Therefore, CD expression
in tumor and stromal cells may serve as an important indicator of
progression and guide postoperative treatment. (C) 1997 Wiley-Liss, Inc
Effect of Turbulence on the Separation of Liquid−Liquid Dispersions in Batch Settlers of Different Geometries
Coalescence and breakage processes
We extend a model for coalescence and breakage of liquid–liquid dispersions proposed by Fasano/Rosso. The main feature is that the experimentally observed fact of a maximal droplet mass is taken into account. Our model includes spontaneous breakage as well as collisional fragmentation. Existence and uniqueness of solutions is proved and the long-time behaviour is investigated. Copyright © 2002 John Wiley & Sons, Ltd
SIGNIFICANCE OF ESTROGEN-RECEPTORS AND CATHEPSIN-D TISSUE DETECTION IN GASTRIC ADENOCARCINOMA
Estrogen receptors (ERs) have recently been reported to be present in
carcinomas of stomach, an organ that has so far been considered as
nontarget for sex hormones. Cathepsin D is an estrogen-regulated
lysosomal protease that has been overexpressed in breast cancer. ER and
cathepsin D immunohistochemical expression were studied in this research
in order to estimate their association to known histopathological and
clinical parameters and their possible prognostic significance as well.
Sixty-two patients with gastric adenocarcinomas were included in this
study. The cancers were studied immunohistochemically concerning ER
positivity in tumor cell nuclei and cathepsin D cytoplasmic expression.
Nuclear ER staining was detected in tumor cells of 25% of male and 27%
of female patients. ER positivity was demonstrated mainly in the well
and moderately differentiated carcinomas; 87.5% of ER(+) tumors were
also characterized as cathepsin D positive and a significant correlation
between ER and cathepsin D positive expression was demonstrated (P <
0.05). Cytoplasmic cathepsin D expression was observed in carcinomatous
cells of 70.9% of gastric tumors. Early tumor stage and good
differentiation were significantly associated with increased cathepsin D
expression (P < 0.05, P < 0.001). Histologic type, degree of
differentiation and tumor stage were significantly correlated to
survival (P < 0.05, P < 0.001 and P < 0.001). The patients who were
cathepsin D(+) had a significant prognostic advantage over the cathepsin
D(-) patients (P < 0.001).
The presence of ER and estrogen-regulated cathepsin D indicates the
involvement of sex hormonal factors in these tumors and cathepsin D
positive expression in tumor cells seems to be related to better
prognosis. Their biological, clinical, and prognostic roles remain to be
further elucidated. (C) 1995 Wiley-Liss, Inc
Modified Bascom's asymmetric midgluteal cleft closure technique for recurrent pilonidal disease - Early experience in a military hospital
Effects of caspase-9 and survivin gene polymorphisms in pancreatic cancer risk and tumor characteristics
Objectives: This case-control study was performed to evaluate the association between a specific caspase-9 polymorphism as well as the genetic polymorphism -31G/C located in the cycle-dependent elements/cell cycle homology regions repressor element of the human survivin promoter and the risk of pancreatic cancer. Methods: Eighty patients with pancreatic cancer and 160 healthy controls were investigated for genotype and allelic frequencies of caspase-9 1263A/G and survivin -31G/C polymorphisms by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism. Results: The G carrier group of patients and the G allele of caspase-9 1263A/G were overrepresented among the pancreatic cancer cases. With regard to tumor characteristics, a statistically significant association was detected between the survivin C carrier group of patients and the advanced T stage as well as the presence of lymph node metastasis. Conclusions: The caspase-9 G allele confers increased susceptibility to pancreatic cancer development, and the survivin C carriage status may be related to aggressive features of this malignancy. Copyright © 2010 by Lippincott Williams & Wilkins