IN VITRO EVALUATION OF A DIET SUPPLEMENTED WITH Mn ON NUTRIENT DIGESTIBILITY AND RUMEN FERMENTATION PATTERN IN CATTLE

An experiment was conducted in 3 male rumen fistulated cattle fed on rice straw and concentrate based total diet to study the effects of supplementation of Manganese (Mn) from inorganic source (manganese sulphate) on in vitro dry matter (DM), organic matter (OM) & neutral detergent fibre (NDF) digestibility (%) & in vitro ammonia nitrogen (NH3-N) & total volatile fatty acid (VFA) production. These animals were offered total diet supplemented with 0 ppm Mn (T0) and 24 ppm Mn (T1) as per NRC, 2001 from inorganic source like manganese sulphate. After 21 days of feeding, rumen liquor samples were drawn at different time intervals (24 hour & 48 hour) to study the digestibility of DM, OM & NDF. For estimation of in vitro ammonia nitrogen (NH3-N) & total volatile fatty acid (TVFA) concentration each sample were incubated for 2 hour, 4 hour, 12 hour, 24 hour & 48 hour interval. This study inferred that supplementation of Mn through feed can improve the digestibility and rumen fermentation pattern and there will be more utilization of nutrient (crude protein, ether extract, organic matter). Trace mineral like Mn at per NRC (2001) recommendation was found more effective for the purpose than without supplementation in a diet

Perfect Teleportation and Superdense Coding With W-States

True tripartite entanglement of the state of a system of three qubits can be classified on the basis of stochastic local operations and classical communications (SLOCC). Such states can be classified in two categories: GHZ states and W-states. It is known that GHZ states can be used for teleportation and superdense coding, but the prototype W-state cannot be. However, we show that there is a class of W-states that can be used for perfect teleportation and superdense coding.Comment: 9 pages, no figur

ANALYSIS OF AGRO-ECOLOGICAL SITUATION FOR IDENTIFICATION OF PROBLEMS BY PRA TECHNIQUES IN ADAPTIVE VILLAGE OF KRISHI VIGYAN KENDRA UNDER NEW ALLUVIA ZONE OF MURSHIDABAD DISTRICT OF WEST BENGAL

Agro Ecosystem analysis using the Participatory Rural Appraisal (PRA) techniques of an adaptive village (Jainpur) of New Alluvial Zone of Murshidabad-Jiaganj block in Murshidabad district, West Bengal revealed that the village basically has rice and jute based farming system. The cropping intensity of the village is 233%. Out of 363 household 80% is engaged in Agriculture, Animal Husbandry and other allied activities. The land availability per household is 0.40 ha. The villagers are mostly scheduled caste. By snow ball technique major problems were identified .On the basis of bio-physical and socio-economic problems, thrust area were selected. Area specific On Farm Trials (OFT) in farmers' were conducted on some researchable issues. Front Line Demonstration (FLD), training programme, health camp, awareness camp and other different extension activities were arranged to mitigate the problems

Isolation and Purification of C-phycocyanin From Nostoc Muscorum (Cyanophyceae and Cyanobacteria) Exhibits Antimalarial Activity in Vitro

The phycobilin pigments are intensively fluorescent and water soluble. They are categorized into three types, such as pigments containing high, intermediate and low energies are phycoerythrins (phycoerythrocyanins), phycocyanins and allophycocyanins, respectively. Besides light harvesting, the phycobiliproteins have shown industrial and biomedical importance. Among them, C-phycocyanin (C-PC) has been considered to be the most preferred one. The present study was undertaken to evaluate the antimalarial activity of C-PC isolated from a nitrogen-fixing cyanobacterium and Nostoc muscorum. C-PC was extracted and purified by acetone extraction and ammonium sulfate precipitation and dialysis followed by amicon filtration. It was isolated as a~124 kDa water soluble protein molecule. It showed antimalarial activity in vitro against chloroquine sensitive and resistant Plasmodium falciparum strains. Inhibitory concentrations at 50%, 90% and 95% were determined as 10.27±2.79, 53.53±6.26 and 73.78±6.92 µg/ml against the chloroquine-sensitive strains; 10.37±1.43, 56.99±11.07 and 72.79±8.59 µg/ml against chloroquine resistant of Plasmodium falciparum strains. C-PC was found to have antimalarial activity even at a concentration of 3.0µg/ml. The possible mechanism might be relied on the destruction of polymerization of haemozoin by binding of C-PC with ferriprotoporphyrin-IX at the water surface of the plasma membrane

Isolation and Purification of C-phycocyanin From Nostoc Muscorum (Cyanophyceae and Cyanobacteria) Exhibits Antimalarial Activity in Vitro

The phycobilin pigments are intensively fluorescent and water soluble. They are categorized into three types, such as pigments containing high, intermediate and low energies are phycoerythrins (phycoerythrocyanins), phycocyanins and allophycocyanins, respectively. Besides light harvesting, the phycobiliproteins have shown industrial and biomedical importance. Among them, C-phycocyanin (C-PC) has been considered to be the most preferred one. The present study was undertaken to evaluate the antimalarial activity of C-PC isolated from a nitrogen-fixing cyanobacterium and Nostoc muscorum. C-PC was extracted and purified by acetone extraction and ammonium sulfate precipitation and dialysis followed by amicon filtration. It was isolated as a~124 kDa water soluble protein molecule. It showed antimalarial activity in vitro against chloroquine sensitive and resistant Plasmodium falciparum strains. Inhibitory concentrations at 50%, 90% and 95% were determined as 10.27±2.79, 53.53±6.26 and 73.78±6.92 µg/ml against the chloroquine-sensitive strains; 10.37±1.43, 56.99±11.07 and 72.79±8.59 µg/ml against chloroquine resistant of Plasmodium falciparum strains. C-PC was found to have antimalarial activity even at a concentration of 3.0µg/ml. The possible mechanism might be relied on the destruction of polymerization of haemozoin by binding of C-PC with ferriprotoporphyrin-IX at the water surface of the plasma membrane

Robotic intraoperative tracheal repair during retrosternal malignant goiter excision: an anesthetic challenge

Thyroidectomy is the treatment of choice for large retrosternal goiters. These tumors often are large enough to require thoracotomy. Robotic surgery can help in avoiding thoracotomy and its associated post operative complications. Rarely, such tumors and their surgery can be complicated by tracheal tears. Such tears, especially those near the carina are difficult to repair and often require open thoracotomy. We described such a case where we avoided open thoracotomy and instead performed a minimally invasive robotic repair of tracheal tear. The maintenance of ventilation during this robotic repair was critical. A combination of several airway devices was used to allow the complex surgical repair to be executed without affecting ventilation

Ball valving laryngeal masses: a difficult “ball” in the anaesthetists’ court

Dynamic airway obstruction (also called ball-valve effect) is a serious condition often seen in glottic lesions. This is an anesthetic challenge as total airway obstruction can occur after the induction of general anesthesia. We present a case of a middle aged female who had a large laryngeal mass which was showing ball-valve effect. The patient had refused for tracheostomy despite it being the safest procedure in the circumstances. We then performed videolaryngoscopy and managed to intubate the patient with the help of some improvisation. The patient underwent a succesful and uneventful surgery