In vivo efficacy of natural essential oil of Syzygium aromaticum L. bud for protecting the Pisum sativum L. seeds

The mycological investigations on sixty samples of stored garden pea food seeds revealed presence of twelve and ten species of fungi by blotter and agar plate techniques respectively. The fungal species were associated with genera viz., Alternaria, Aspergillus, Botrytis, Chaetomium, Cladosporium, Curvularia, Fusarium, Penicillium and Rhizopus. The fungal species viz., Cladosporium herbarum (Pers.) Link and Penicillium italicum Wehmer did not show its appearance in agar plate method. It showed dominance of Aspergillus flavus, Aspergillus niger, Aspergillus ochraceous, Aspergillus terreus in blotter method in comparison to agar plating. Aspergillus tmarii, P. italicum and Rhizopus stolonifer did not grow on sterilized seeds in Blotter method. Pathogenicity tests of dominant fungi caused biodeterioration in garden pea seeds. The antifungal testing of essential oils revealed Syzygium aromaticum L. bud oil to be fungitoxic at 500 ppm (0.025 mL). The minimum inhibitory concentration of the S. aromaticum oil was found to be 300 ppm against four fungi viz., A. flavus, A. niger, A. ochraceous and A. terreus. At this concentration the oil was found to be fungicidal and thermostable. The oil activity was not affected by physical factors and showed broad spectrum. In vivo study depicted that clove oil was more effective in comparison to EDCT. It controlled a maximum of 4 fungi while the clove oil showed no growth of fungi even after 6 months storage

Fabrication of α‑Fe2O3 Nanostructures: synthesis, characterization, and their promising application in the treatment of Carcinoma A549 Lung Cancer Cells

In the present work, iron nanoparticles were synthesized in the α-Fe2O3 phase with the reduction of potassium hexachloroferrate(III) by using l-ascorbic acid as a reducing agent in the presence of an amphiphilic non-ionic polyethylene glycol surfactant in an aqueous solution. The synthesized α-Fe2O3 NPs were characterized by powder X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, atomic force microscopy, dynamic light scattering, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and ultraviolet–visible spectrophotometry. The powder X-ray diffraction analysis result confirmed the formation of α-Fe2O3 NPs, and the average crystallite size was found to be 45 nm. The other morphological studies suggested that α-Fe2O3 NPs were predominantly spherical in shape with a diameter ranges from 40 to 60 nm. The dynamic light scattering analysis revealed the zeta potential of α-Fe2O3 NPs as −28 ± 18 mV at maximum stability. The ultraviolet–visible spectrophotometry analysis shows an absorption peak at 394 nm, which is attributed to their surface plasmon vibration. The cytotoxicity test of synthesized α-Fe2O3 NPs was investigated against human carcinoma A549 lung cancer cells, and the biological adaptability exhibited by α-Fe2O3 NPs has opened a pathway to biomedical applications in the drug delivery system. Our investigation confirmed that l-ascorbic acid-coated α-Fe2O3 NPs with calculated IC50 ≤ 30 μg/mL are the best suited as an anticancer agent, showing the promising application in the treatment of carcinoma A549 lung cancer cells

Deciphering of seed Health of common food grains (wheat, rice) of North Eastern UP and Gurgaon Haryana, India

Abstract The stored random samples of food seeds of wheat and rice (60 samples) were purchased from places of Eastern UP and Gurgaon district Haryana. Its moisture contents were estimated. The Mycological investigations of wheat seeds revealed presence of a total number of 16 fungal species viz., Alternaria alternata, Aspergillus candidus, Aspergillus flavus, A. niger, A. ochraceous, A. phoenicis, A. tamari, A. terreus, A. sydowi, Fusarium moniliforme, F. oxysporum F. solani, P. glabrum, Rhizopus nigricans, Trichoderma viride and Trichothecium roseum. While mycological analysis of rice seeds showed presence of 15 fungal species viz., Alternaria padwickii, A. oryzae, Curvularia lunata, Fusarium moniliforme, Aspergillus clavatus, A. flavus, A. niger, Cladosporium sp., Nigrospora oryzae, Alternaria tenuissima, Chaetomium globosum, F. solani, Microascus cirrosus, Helminthosporium oryzae, Pyricularia grisea. It also projected variation in presence of fungal species in blotter and agar plate method of analysis. In wheat Blotter method of analysis showed 16 fungal species while agar plate depicted 13 fungal species. In rice Agar plate method depicted presence of 15 fungal species while blotter method shows presence of 12 fungal species. The insect analysis revealed that wheat samples were infected with Tribolium castaneum. While rice seeds sample showed presence of insect Sitophilus oryzae. The investigations revealed that Aspergillus flavus, A. niger, Sitophilus oryzae and Tribolium castaneum caused reduction in seed weight loss, seed germination, carbohydrate and protein contents of common food grains (wheat, rice). It also revealed that randomly selected A. flavus isolate 1 of wheat showed higher potential of aflatoxin B1 production (1392.940 μg/l) while rice isolate 2 showed 1231.117 μg/l production

In vivo efficacy of natural essential oil of Syzygium aromaticum L. bud for protecting the Pisum sativum L. seeds

383-390The mycological investigations on sixty samples of stored garden pea food seeds revealed presence of twelve and ten species of fungi by blotter and agar plate techniques respectively. The fungal species were associated with genera viz., Alternaria, Aspergillus, Botrytis, Chaetomium, Cladosporium, Curvularia, Fusarium, Penicillium and Rhizopus. The fungal species viz., Cladosporium herbarum (Pers.) Link and Penicillium italicum Wehmer did not show its appearance in agar plate method. It showed dominance of Aspergillus flavus, Aspergillus niger, Aspergillus ochraceous, Aspergillus terreus in blotter method in comparison to agar plating. Aspergillus tmarii, P. italicum and Rhizopus stolonifer did not grow on sterilized seeds in Blotter method. Pathogenicity tests of dominant fungi caused biodeterioration in garden pea seeds. The antifungal testing of essential oils revealed Syzygium aromaticum L. bud oil to be fungitoxic at 500 ppm (0.025 mL). The minimum inhibitory concentration of the S. aromaticum oil was found to be 300 ppm against four fungi viz., A. flavus, A.niger, A. ochraceous and A. terreus. At this concentration the oil was found to be fungicidal and thermostable. The oilactivity was not affected by physical factors and showed broad spectrum. In vivo study depicted that clove oil was moreeffective in comparison to EDCT. It controlled a maximum of 4 fungi while the clove oil showed no growth of fungi evenafter 6 months storage