KORELASI AKTIVITAS ADENOSINE DEAMINASE ANTARA CAIRAN PLEURA DAN SERUM PADA PENDERITA DENGAN EFUSI PLEURA

Pleural effusion is an abnormal accumulation of fluid in the pleural space resulting from increased production of fluid or decrease resorption of fluid in the pleural space. Pleural effusion can be caused by infectious diseases, malignancies, collagen disease, gastrointestinal disease, heart disease, and other causes such as medication, nephrotic syndrome, and radiation. Adenosine deaminase (ADA) is an enzyme involved in the catabolism of purines which catalysis the change of adenosine into inosine and deoxyadenosine into deoxyinosine. This enzyme can be measured in pleural fluid, serum and other body fluids such as cerebrospinal and ascites fluid. The aim of this study was to analyze the correlation between adenosine deaminase activity in pleural fluid and serum in patients with pleural effusion. Methods. Subjects were 46 patients with various causes of pleural effusion. This research was an analytical observational study with a cross sectional design. Examination of ADA activity was performed in pleural fluid and serum. ADA activity was examined using enzymatic methods, using Diazyme reagent by TMS 24i Premium. Results. Mean±SD ADA activity for all pleural effusion sample in serum was 13.037 8.365 U//L and pleural fluid 30.843 28.860 U//L. No correlation between ADA activity in serum and pleural fluid (r=0.173,p= 0.252) in all sample. No correlation between ADA activity in serum and pleural fluis was found in malignancies (r=0.109, p=0.630), tuberculosis (r= 0.366,p = 0.123), systemic diseases (r =0.466, p=0.429), and non tuberculosis group (r=0.126,p=0.532). Conclusion. There was an increase of ADA activity in pleural fluid. There was no significant correlation between pleural fluid ADA activity and serum

CORRELATION BETWEEN ADENOSINE DEAMINASE ACTIVITY IN PLEURAL FLUID AND SERUM OF PATIENTS WITH PLEURAL EFFUSION

Pleural effusion is an abnormal accumulation of fluid in the pleural space resulting from increased production of fluid or decreased resorption of fluid in the pleural space. Pleural effusion can be caused by infectious diseases, malignancies, collagen disease, gastrointestinal disease, heart disease and other causes such as medication. Adenosine Deaminase (ADA) is an enzyme involved in the catabolism of purines. This enzyme can be measured in pleural fluid, serum and other body fluids such as cerebrospinal and ascites fluid. The aim of this study was to analyze the correlation between adenosine deaminase activity in pleural fluid and serum in patients with pleural effusion. This research was an observational study with a cross-sectional design. Examination of ADA activity was performed in pleural fluid and serum. Adenosine deaminase activity was examined using photometric methods (Non-Giusti), using Diazyme reagent by TMS 24i Premium. Subjects were 46 patients with pleural effusion caused by malignancies, tuberculosis and systemic diseases. Mean±SD ADA activity for all pleural effusion samples in serum was 13.037± 8.365 (0.5-34.1) U//L and pleural fluid 30.843± 28.860 U//L (1.3-140.8). No correlation between ADA activity in serum and pleural fluid (r=0.173, p= 0.252) was found in all samples. No correlation between ADA activity in serum and pleural fluis was found in malignancies (r=0.109, p=0.630), tuberculosis (r= 0.366, p=0.123), systemic diseases (r =0.466, p=0.429) and non-tuberculosis group (r=0.126, p=0.532). There was no correlation between pleural fluid ADA activity and serum

CRITICAL VALUE OF GLUCOSE USING CRITIVA 1.0 APPLICATION IN BALI MANDARA EYE HOSPITAL

Critical value is very critical result in laboratory examination that can indication several emergency conditions. Reporting critical values are controversial between hospital management, clinicians, and laboratory. Reporting critical value required effective communication. Information technology has become one of the solution effective communication in reporting critical values. Critical values can occur in clinical chemistry, hematology, and other laboratory examination. Glucose is one of the clinical chemistry parameters which is often foound critical value and therapy must be given immediately. Aim of this research is to evaluated time reporting using Critiva 1.0 application in cellular phone of clinical pathologist Bali Mandara Eye Hospital. Samples were 50 times reporting the glucose critical value simulation using the Critiva 1.0 application and 50 times reporting the Standard Operating Operations (SPO) simulation at the Bali Mandara Eye Hospital. Simulation carried by laboratory techonology in charge. We used means to analyze time reporting and SPSS version 16. Mean reporting glucose critical value using Critiva 1.0 application was 12 seconds. Mean reporting glucose critical value using SPO Bali Mandara Eye Hospital was 720 seconds

Hematological profile of Patients in Udayana University General Hospital

Background: In medical patients, the single most common investigation is carried out by means of the full blood count (FBC). This is very helpful in providing information for diagnosis and management, if interpreted carefully and in relation to clinical history. Primary hematological disorders by abnormalities in FBC are often beware by doctors. Method: We performed hematology laboratory from May 2019 until October 2019 in all of patient who administered in Udayana University General Hospital. From the recruited participants who were directly collected data demographics and information about drug history. Results: In present study out of 450 cases, 205 (45.6%) were males and 245 (54.4%) were females. It was found from different department, 71% from general practitioner, 14.5% from internal medicine, 6.9% from obstetric and gynecology department, and 4.9% from pediatric department. We found 7.1% cases of anemia, 34.9% cases of leukocytosis, 9.8% cases of leukopenia, and 16.9% cases of thrombocytopenia. Conclusion: Important information sourced from FBC can help doctors in patient diagnosis and management. In relation to diagnosis, surgical intervention and drug treatment it is very important to value not only the current lab results but also to assign potential trends over time

The relationship between Epidermal Growth Factor Receptor (EGFR) mutation and computed tomography findings in lung adenocarcinoma

Background: Adenocarcinoma is the highest histology subtype of lung cancer. Epidermal Growth Factor Receptor (EGFR) is a factor that can predict the prognosis or response to treatment in patients with lung adenocarcinoma. Adenocarcinomas with EGFR mutations generally have a component of Ground Glass Opacity (GGO), smaller size, oval shape, multiple nodal metastases, and distant metastases.  The aim of this study was to determine the relationship between EGFR mutations and CT scan images in pulmonary adenocarcinoma patients. Methods: This was a retrospective analytical study consisting of 92 samples, which were categorized into the mutation group (n = 62) and wild group (n = 30). This study was conducted at Dr. Soetomo General Hospital from January 2015 – December 2017. The assessed CT-Scan findings were tumor size, shape, density, lymph node size, pleural effusion, and metastasis presence. The relationship was analyzed using the chi-square test and considered significant if the p-value was < 0.05. Results: This study found a significant relationship between EGFR mutations status with tumor size ≤ 3 cm (p = 0.02), lymph node size < 1.5 cm (p = < 0.001) and metastasis (p = 0.026). However, tumor density, tumor form, and pleural effusion did not have significant relationship with EGFR mutation. Conclusion: Tumor size ≤ 3 cm, lymph node size < 1.5 cm and presence of metastasis can be found in EGFR mutations pulmonary adenocarcinoma patients

Serum Hepcidin Level in Patients with Acute Lymphoblastic Leukemia (ALL) during The Treatment Phase: Their Effects on Erythropoisis Activity and Iron Reserves

Hepcidin levels increased significantly with increased of iron stores in early phase of acute leukemia patients when erithropoisis pressed by blast cells in bone marrow, then decreased significantly with acute leukemia remission. The study aimed to determine role of hepcidin in activity of erythropoisis and serum iron reserves in acute lymphoblastic leukemia (ALL) patients during phase of therapy. This study used an observational analytic design. Serum hepcidin examination used ELISA methods. Erythropoisis activity was determined by complete blood and reticulocyte percentage. Iron reserves was determined by serum iron and ferritin levels by ECLIA. Result shows a total of 48 patient subjects were divided into groups of induction, consolidation and maintenance phase with an average age of 6.81 years (induction), 9.7 years (consolidation) and 7.8 years (maintenance). In the normality test with Shapiro-Wilk data showed abnormal distribution (p <0.05). Analysis by the Kruskal-Wallis test showed there were differences between the three treatment phases in the examination of hemoglobin, reticulocytes, serum iron, ferritin and hepcidin (p <0.05). In statistical analysis with Spearman's rank correlation shows there is a significant correlation between hemoglobin with ferritin (r = -0.416, p = 0.003), hemoglobin with hepcidin (r = -0.305, p = 0.035), reticulocytes with hepcidin (r = -0.496), p = 0.000) and serum iron with hepcidin (r = -0.302, p = 0.037). We concluded that the higher levels of hepcidin indicate lower levels of hemoglobin, reticulocytes and serum iron in patients with acute lymphoblastic leukemia during treatment phase