STRUCTURAL UNDERSTANDING OF CYTOTOXIN 1 OF NAJA SPUTATRIX: A POTENTIAL ANTICANCER AGENT

Snake venom cytotoxin from different Naja species possesses significant cytotoxic activity on tumor cells. The cytoxin from snake venom can exert a plethora of biological activities including depolarization and muscular cell contraction, lysis of variety of cells such as red blood cells, epithelial cells and fetal lung cells, and also apoptotic activity on certain types of tumor cells. In the present article, we have effectively utilized comparative modeling approach to propose the first molecular model structure of cytotoxin 1 of Naja sputatrix.  The charge distribution on the structure and distribution of secondary structural elements were also investigated with the aid of In silico based approach. A homology structural model of the protein was generated and analyzed to deduce molecular enrichment strategy. The model data and other relevant post model analysis data provides a clear understanding of molecular structure of cytotoxin 1 of Naja sputatrix and its relevant cytotoxic potential for the development of a beneficial anticancer natural lead compound. Keywords: Cytotoxin 1, Naja Sputatrix, Anticancer Agent, Snake Venom, Structural model, Malayan cobra

The saga of cytotoxin evolution–Switching of destructive role to a constructive role

259-265Snake venom contains the toxin proteins, cytotoxins. Cytotoxins exert their effect upon the target cells by interacting with membrane lipids and proteins. Ultimate objective of a cytotoxin is to destroy the target cells. These cytotoxins contain cysteine residues responsible for disulphide linkage between them. Similar variety of peptides enriched with cysteine is also found in many other organisms. But interestingly, in those cases they never have a cell destructive function, in turn, they act to be cell-friendly. In this work, we analysed the cytotoxins and related peptides in terms of amino acid percentage profile, multiple sequence alignment, codon usage, isoelectric point determination, protein secondary structure prediction and phylogenetic tree construction through different softwares. Among all the interesting results, lysine profile was very much informative. High amounts of lysine are conserved in all the cytotoxins whereas in other related peptides it is in less numbers. Phylogenetic tree showed a stepwise dynamic evolution of these interesting molecules. This paper, therefore, showed that there is a great possibility to turn harmful natural peptides into a beneficial engineered molecule for the betterment of lives of mankind