RNA Helicase DDX5 Regulates Platelet Derived Growth Factor Receptor β and Androgen Receptor (AR) Expression in Breast Cancer

P68 RNA helicase, a prototypical member of the DEAD box family of RNA helicases is important in many biological processes, including early organ development. However, its aberrant expression contributes to tumor development and progression. In this study, we show that p68 upregulates the transcriptional expression of the growth factor receptor, PDGFRb P68 knockdown in MDAMB231 and BT549 breast cancer cells significantly decreases mRNA and protein levels of PDGFRb and EMT markers, resulting in decreased migration. We have previously shown that PDGF-BB induces p68 phosphorylation, resulting in EMT via nuclear translocation of b -catenin. Here, we show that p68 promotes migration in response to PDGF-BB stimulation via upregulation of PDGFRb in breast cancer cells, suggesting that PDGFRb is in turn regulated by p68 to maintain a positive feedback loop. Further, our study reveals the association of p68 in androgen receptor (AR) response via PDGFRb. We demonstrate that p68 and PDGFRb co-regulate AR expression and promote androgen-mediated proliferation in breast cancer cells, highlighting the critical role of p68-PDGFRb axis in AR regulatio

RNA HELICASE DDX5 REGULATES PLATELET DERIVED GROWTH FACTOR RECEPTORAND ANDROGEN RECEPTOR (AR) EXPRESSION IN BREAST CANCER

P68 RNA helicase, a prototypical member of the DEAD box family of RNA helicases is important in many biological processes, including early organ development. However, its aberrant expression contributes to tumor development and progression. In this study, we show that p68 upregulates the transcriptional expression of the growth factor receptor, PDGFRb P68 knockdown in MDAMB231 and BT549 breast cancer cells significantly decreases mRNA and protein levels of PDGFRb and EMT markers, resulting in decreased migration. We have previously shown that PDGF-BB induces p68 phosphorylation, resulting in EMT via nuclear translocation of b -catenin. Here, we show that p68 promotes migration in response to PDGF-BB stimulation via upregulation of PDGFRb in breast cancer cells, suggesting that PDGFRb is in turn regulated by p68 to maintain a positive feedback loop. Further, our study reveals the association of p68 in androgen receptor (AR) response via PDGFRb. We demonstrate that p68 and PDGFRb co-regulate AR expression and promote androgen-mediated proliferation in breast cancer cells, highlighting the critical role of p68-PDGFRb axis in AR regulatio

Biochemical mapping of the measles virus H and F envelope glycoprotein protein-protein interface

The Paramyxoviridae family includes several viruses that are important to human health, including measles virus. The envelope glycoproteins are essential for attachment and entry of the virus into the host cell [1, 2]. To develop novel therapeutics against the virus, detailed knowledge of envelope glycoprotein protein-protein interaction is important. The goal of this study is to characterize the MeV entry machinery on a molecular level. Interaction of haemaglutinin (H) and fusion (F) protein in pre-fusion form can be biochemically detected with DTSSP. To map the interaction site of H and F protein in pre-fusion form, we have mutated lysine (K) to arginine (R) in the F protein, and examined surface expression. The mutated F was still expressed on the surface and amount of surface expression correlated with fusion activity. The altered F proteins produced in this study will be used to further characterize the H-F interaction

Importance of language skill learning of dental undergraduates: need assessment and remediation in India

For students entering health education, it is essential to learn the languages that are native to the place of education. The study is undertaken with purpose to assess language skill of undergraduate students at Sharad Pawar Dental College in India so that remedying can be planned at their entry to the new course. This cross-sectional study was done from September 2014 to February 2015. The 157 dental undergraduates were subjected to structured questionnaire that has items on their assessment of three languages i.e., Marathi, Hindi and English. Later, the need assessment to develop language skills of students was done through focus group discussions. Students` perception about language classes was obtained through interviews. The correct response rate of study participants to test items on three languages ranged from 69.4%-81.05% and 82.5%-91.59% for first and second year, respectively. There were significant differences in response rate among three language item groups. The language classes brought out appreciable changes in their understanding of local languages. Study brought out need to address language gaps to aid to smooth out their transitions in new institute

Realization of Advanced Encryption Standard for Power and Area optimization

An AES algorithm can be implemented in software or hardware but hardware implementation is more suitable for high speed applications.AES is most secure security algorithm to maintain safety and reliability of date transmission for this key size is important. And here used AES-256 bit.The main goal of paper is AES hardware implementation to achieve less area and low power consumptions also to achieve high speed data processing and reduce time for key generation. This paper presents AES-256 bit algorithm design consist of 128 bit symmetric key. Xilinx ISE.14.7(64-bit) is used for simulation by using VHDL and hardware implementation on FPGA(Xilinx Spartan 6 or Altera Cyclone 2 FPGA device)

PERFORMING MULTIPLICATIONS IN IMAGE FILTERING PROCESS USING VEDIC MATHEMATICS

Image filtering is a very important step in image processing. Filtering involves lots of multiplications which consumes time. Time required increases with the increase in the number of pixels. This paper proposes an approach for image filtering using Vedic Mathematic which performs faster multiplication compared to the conventional algorithms namely Booth 2, Booth 3 and Array Multiplication Algorithm thus reducing the time required for filtering of images. Experimentation is done using C language. Time required by the algorithms for filtering are then compared using the experimental results

Effect of Oral Submucous Fibrosis on Jaw Dimensions

Objective:The disuse of the jaws owing to the restricted movement of the mandible in advanced cases of oral submucous fibrosis (OSMF) may have an effect on the morphologic features of the jaw bones. The purpose of the present study was to determine the jaw bone measurements in patients with OSMF and to compare the measurements in normal subjects and Caucasian norms.Methods:The lateral cephalograms of 59 subjects (43 males and 16 females) with OSMF and 44 normal subjects (23 males and 21 females) in the age group of 18–45 years were collected. The jaw measurements were performed using Burstone analysis, and the relevant linear measurements of jaw sizes included were N-A, N-B, N-ANS, ANS-PNS, ANS-Gn, Ar-Go, and Pg-Go. The sex-wise comparison was performed using unpaired t-test, and measurements were compared with other studies using Z test.Results:In the present study, sex-wise comparison was found to be significant with greater jaw measurements in males than in females in patients with OSMF. Overall, the measurements were less or equal in patients with OSMF than in normal subjects except for N-ANS and Pg-Go. When study measurements were compared with Burstone measurements, differences were significant with greater and less measurements.Conclusion:Overall, the jaw measurements were less or equal in patients with OSMF than in normal subjects except for mandibular body length and middle third facial height that may need further evaluation.Objective:The disuse of the jaws owing to the restricted movement of the mandible in advanced cases of oral submucous fibrosis (OSMF) may have an effect on the morphologic features of the jaw bones. The purpose of the present study was to determine the jaw bone measurements in patients with OSMF and to compare the measurements in normal subjects and Caucasian norms.Methods:The lateral cephalograms of 59 subjects (43 males and 16 females) with OSMF and 44 normal subjects (23 males and 21 females) in the age group of 18–45 years were collected. The jaw measurements were performed using Burstone analysis, and the relevant linear measurements of jaw sizes included were N-A, N-B, N-ANS, ANS-PNS, ANS-Gn, Ar-Go, and Pg-Go. The sex-wise comparison was performed using unpaired t-test, and measurements were compared with other studies using Z test.Results:In the present study, sex-wise comparison was found to be significant with greater jaw measurements in males than in females in patients with OSMF. Overall, the measurements were less or equal in patients with OSMF than in normal subjects except for N-ANS and Pg-Go. When study measurements were compared with Burstone measurements, differences were significant with greater and less measurements.Conclusion:Overall, the jaw measurements were less or equal in patients with OSMF than in normal subjects except for mandibular body length and middle third facial height that may need further evaluation

Streaming Video QoE Modeling and Prediction: A Long Short-Term Memory Approach

HTTP based adaptive video streaming has become a popular choice of streaming due to the reliable transmission and the flexibility offered to adapt to varying network conditions. However, due to rate adaptation in adaptive streaming, the quality of the videos at the client keeps varying with time depending on the end-to-end network conditions. Further, varying network conditions can lead to the video client running out of playback content resulting in rebuffering events. These factors affect the user satisfaction and cause degradation of the user quality of experience (QoE). It is important to quantify the perceptual QoE of the streaming video users and monitor the same in a continuous manner so that the QoE degradation can be minimized. However, the continuous evaluation of QoE is challenging as it is determined by complex dynamic interactions among the QoE influencing factors. Towards this end, we present LSTM-QoE, a recurrent neural network based QoE prediction model using a Long Short-Term Memory (LSTM) network. The LSTM-QoE is a network of cascaded LSTM blocks to capture the nonlinearities and the complex temporal dependencies involved in the time varying QoE. Based on an evaluation over several publicly available continuous QoE databases, we demonstrate that the LSTM-QoE has the capability to model the QoE dynamics effectively. We compare the proposed model with the state-of-the-art QoE prediction models and show that it provides superior performance across these databases. Further, we discuss the state space perspective for the LSTM-QoE and show the efficacy of the state space modeling approaches for QoE prediction

Outcomes of retropupillary iris-claw lens implantation

Background: Inadequate posterior capsular support during cataract surgery makes it difficult to implant posterior chamber intraocular lens.One of the secondary intraocular lens implantation options is iris claw lens.Retropupillary iris claw lens is preferred than anterior because chances of damage to the anterior chamber angle and iris root are avoided as the lens is fixed to the mid periphery of iris. The prospective study aims to analyse the visual outcomes and immediate postoperative complications after retropupillary iris-claw lens implantation in a tertiary care hospital. Methods: It was a prospective study done conducted on 24 patients with inadequate posterior capsular support in which retropupillary iris-claw lens implantation was done from December 1, 2021 to May 31, 2022 at Government Regional Eye Hospital, Visakhapatnam. Immediate postoperative complications were noted. Final visual outcomes were noted after 2 months. Results: The study comprised of 24 eyes. The mean patient age was 55.91 years (range 44-77 years).The IOLs were inserted during primary lens surgery in 16 eyes (66.6%) and as a secondary procedure in 8 aphakic eyes (33.4%). The mean postoperative intraocular pressure was 15.1 mmHg. Majority of the eyes had best corrected visual acuity of 6/12 (41.6%).The most common immediate postoperative complication was pupil ovalization (25%) and striate keratopathy (25%). Least common complication was choroidal detachment (3.6%). Conclusions: Visual outcomes of primary and secondary iris claw implantation were similar. The retro-pupillary iris-claw lens implantation provided good visual outcome with tolerable complications. It can be used in eyes with inadequate posterior capsular support