Courtship, mating and feeding data for observations during the virgin and non-virgin matings combined (observation points 1 to 7).

<p>See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002187#s4" target="_blank">Methods</a> for details.</p

Number of individuals present at the yeast food source (± s.e.) when ML chromosomes (shaded bars) and C chromosomes (open bars) were expressed in females (left side) and males (right side) in vials containing individuals of both sexes.

<p>Number of individuals present at the yeast food source (± s.e.) when ML chromosomes (shaded bars) and C chromosomes (open bars) were expressed in females (left side) and males (right side) in vials containing individuals of both sexes.</p

Courtship, mating and feeding data for observations during the virgin and non-virgin matings combined (observation points 3 to 7).

<p>See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002187#s4" target="_blank">methods</a> for details.</p

Total number of matings observed (± s.e.) per four pairs in a vial when male-limited evolved chromosomes (shaded bars) and C (open bars) are expressed in females (left side) and in males (right side).

<p>No significant differences in mating rate were detected between selection treatments in either sex. Because of the spot check strategy employed (checks every hour, with mating lasting 17 minutes, on average), mating rate estimates are several times lower than real mating rates.</p

Pathway results for Danish sister pairs (n = 93); showing only genes related to the Discovery findings.

<p>Pathway results for Danish sister pairs (n = 93); showing only genes related to the Discovery findings.</p

Most significant pathway results for Finnish mothers with recurrent SPTB (n = 10).

<p>Most significant pathway results for Finnish mothers with recurrent SPTB (n = 10).</p

Overview of the whole exome sequencing study workflow.

<p>Abbreviations used QC; quality control, MAF; minor allele frequency, RD; read depth, GQ; genotype quality.</p

Sequencing results and schematic representation of the EKV-1 and -2 genome organization.

<p>(<b>A</b>) Overview of the data generated for each novel rhabdovirus. (<b>B</b>) A schematic showing the assembled genomes, consisting of the following genes: <i>nucleoprotein</i> (N), <i>phosphoprotein</i> (P), <i>matrix</i> (M), <i>U1</i>/<i>U2</i>/<i>U3</i> (uncharacterized accessory proteins), <i>glycoprotein</i> (G), and <i>polymerase</i> (L). We indicate in orange (EKV-1) and blue (EKV-2) segments of the viral genomes that could not be assembled from Illumina reads and instead Sanger sequenced. (<b>C</b>) Coverage plots of the final viral genomes.</p

HSPA1L and GR protein levels in decidualized human endometrial stromal fibroblasts.

<p>Cultured ESFs were transfected with WT or Ala268Thr <i>HSPA1L</i>-pcDNA3.1 constructs or with empty pcDNA3.1 vector (control). Cells were treated with decidualization media supplemented with 100nM dexamethasone (glucocorticoids) for 72h. Both cytosolic and nuclear protein were extracted, and HSPA1L and GR protein levels were measured by Western blot. Band intensity of HSPA1L or GR was normalized to band intensity of the corresponding β-actin. Cytosolic (A) and nuclear (B) HSPA1L levels as well as cytosolic (C) and nuclear (D) GR levels are shown for control (empty vector), WT and Ala268Thr sample groups. Each experiment was performed as triplicates in three different passages (n = 9 each group, except n = 8 for nuclear control group) and bars represent mean + SEM. Significant p-value <0.05 is presented with an asterisk. Cytosolic GR levels were significantly higher (p = 0.04) in the WT compared to the Ala268Thr group as well as in the WT compared to the control group (p = 0.04).</p

Sero-positivity to EKV-1 and EKV-2.

<p>A serosurvey for EKV-1 and EKV-2 was performed on Nigerian samples (n = 320). Cut-off values were based on the mean of US normals (n = 137) plus either 3xSD or 5xSD (SD = standard deviation).</p><p>Sero-positivity to EKV-1 and EKV-2.</p