Effect of knock-down of CTR1 on the sensitivity of cells to cDDP.

<p>(A) Effect of knock-down of CTR1 on the sensitivity of ovary cancer cells to cDDP. OVCAR3 cells were transfected with three company sythetic si-RNAs or siRNA control and the western blost analysis showed si-RNA 3 exhibiting the best effect. After transfected with si-RNA3 or siRNA control, OVCAR3 and SKOV3 cells were treated with cDDP at various doses for 48 h and the cell survival fraction was detected by MTT assay. (B) Embryonic kidney HEK-293 cells were tranfected with human CTR1 si-RNA3 or siRNA control. Then the cells were exposed by indicated doses of cDDP for 48 h and the cell survival fraction was detected by MTT assay. (*<i>P</i><0.05).</p

EGCG inhibits the degredation of CTR1 induced by cDDP.

<p>(A, B) The effect of cDDP on the expression of CTR1 in OVCAR3 and SKOV3 cells. The cells were treated with 10 μM cDDP for the indicated time and CTR1 protein expression were detected by western blot analysis. (C)The effect of MG132 on the degradation of CTR1. After OVCAR3 cells were pretreated with 5 μM MG132 for 10h, the cells were incubation with 10 μM cDDP for 14 h. Then followed by western blot analysis. (D, E) The effect of EGCG on cDDP-trigged decrease of CTR1. The OVCAR3 and SKOV3 cells were treated with/without 10μM EGCG in the presence/absence of 10μMcDDP for 24 h. CTR1 protein expression was detected. The bands were quantified with Image J software. (*<i>P</i><0.05, **<i>P</i><0.01)</p

DataSheet_1_Prognostic value of receptor tyrosine kinases in malignant melanoma patients: A systematic review and meta-analysis of immunohistochemistry.doc

BackgroundSubstantial evidence suggests that receptor tyrosine kinases (RTKs) are overexpressed in tumors; however, few studies have focused on the prognostic value of RTKs in melanoma.ObjectivesThe objective of this study is to evaluate the association between overexpression of RTKs and survival in melanoma patients based on immunohistochemistry (IHC) analysis.MethodsOur review is registered on PROSPERO (http://www.crd.york.ac.uk/PROSPERO), registration number CRD42021261460. Seven databases were searched, and data were extracted. We used IHC to measure the association between overexpression of RTKs and overall survival (OS), disease-free survival (DFS), progression-free survival (PFS), and clinicopathology in melanoma patients. Pooled analysis was conducted to assess the differences between Hazard Ratios along with 95% confidence intervals.ResultsOf 5,508 publications examined following the database search, 23 publications were included in this study, which included data from a total of 2,072 patients. Vascular endothelial growth factor receptor 2 (VEGF-R2) overexpression was associated with worse OS and DFS in melanoma. Furthermore, there was an association between OS and the expression of several RTKs, including epidermal growth factor receptor (EGFR), mesenchymal-epithelial transition factor (MET), vascular endothelial growth factor receptor 1 (VEGF-R1), and insulin-like growth factor 1 receptor (IGF-1R). There were no significant correlations between EGFR overexpression and worse DFS or PFS. EGFR overexpression was associated with worse OS cutaneous and nasal melanoma, but not uveal melanoma. However, MET overexpression was related to worse OS in both cutaneous and uveal melanoma. Furthermore, EGFR overexpression was associated with a worse OS in Europe compared to other geographic areas. Moreover, EGFR and MET overexpression showed significant prognostic value in patients with the cut-off “≥10% staining”.ConclusionsOur findings build concrete evidence that overexpression of RTKs is associated with poor prognosis and clinicopathology in melanoma, highlighting RTK expression has the potential to inform individualized combination therapies and accurate prognostic evaluation.</p

Effect of EGCG on the sensitivity of the ovarian cancer to cDDP.

<p>(A) Effect of EGCG on ovary cancer cells survival fraction. OVCAR3 and SKOV3 cells were treated with the indicated concentrations of EGCG for 24 h, then followed by MTT assay to detect cell the survival fraction. (B) Effect of the combination of EGCG and cDDP on cell survival fraction. The cells were treated with indicated concentration of cDDP alone, or in combination with the EGCG (10 μM, shown as E10), and followed by MTT assay. (C) EGCG in combination with cDDP repressed colony formation. OVCAR3 cells were treated with 10 μM of EGCG alone or in combination of 10 μM of cDDP for 48 h. When the colonies formed two weeks later, colony formation assay was carried out. (D) The combination of EGCG and cDDP on cells apoptosis, Hoechst 33258 staining was used to detect apoptosis caused by the indicated treatments. (*<i>P</i><0.05, **<i>P</i><0.01)</p

Effects of EGCG on Pt and DNA-Pt adducts accumulation in the cells.

<p>OVCAR3 cells were treated with/without 10μM EGCG and 30 μMcDDP for 4 h, and then followed by ICP-MC assay. (A) Whole-cell Pt accumulation. (B) DNA-Pt adducts accumulation. (**<i>P</i><0.01)</p

EGCG enhances the efficacy of cDDP on tumor responsiveness and attenuates the nephrotoxicity induced by cDDP in vivo.

<p>Four groups (control, EGCG, cDDP and EGCG+cDDP) were set up. Except there were 6 mice in control group, there were 8 mice for each of the other groups. The body weight (A) and the tumor size (A) were measured twice a week. (B) The mRNA expression of the CTR1 in tumor tissues was measured by RT-PCR and real qPCR. (C) The expression of CTR1 in tumor tissue was assessed by western blotting. (D) The expression of CTR1 in kidney tissue was measured by western blotting. The bands were quantified by Image J software. (*P<0.05, **P<0.01)</p

Low-Resistance Dual-Purpose Air Filter Releasing Negative Ions and Effectively Capturing PM_2.5

The fatal danger of pollution due to particulate matter (PM) calls for both high-efficiency and low-resistance air purification materials, which also provide healthcare. This is however still a challenge. Herein, a low-resistance air filter capable of releasing negative ions (NIs) and efficiently capturing PM_2.5 was prepared by electrospinning polyvinylidene fluoride (PVDF) fibers doped with negative ions powder (NIPs). The air-resistance of fibrous membranes decreased from 9.5 to 6 Pa (decrease of 36%) on decreasing the average fiber diameter from 1.16 to 0.41 μm. Moreover, the lower rising rate of air-resistance with reduction in pore size, for fibrous membranes with thinner fiber diameter was verified. In addition, a single PVDF/NIPs fiber was provided with strong surface potentials, due to high fluorine electronegativity, and tested using atomic force microscopy. This strong surface potential resulted in higher releasing amounts of NIs (RANIs). Interestingly, reduction of fiber diameter favored the alleviation of the shielding effects on electric field around fibers and promoted the RANIs from 798 to 1711 ions cc^–1. Moreover, by regulating the doping contents of NIPs, the RANIs increased from 1711 to 2818 ions cc^–1. The resultant fibrous membranes showed low air resistance of 40.5 Pa. Field-tests conducted in Shanghai showed stable PM_2.5 purification efficiency of 99.99% at high RANIs, in the event of haze

<i>ErmF</i> and <i>ereD</i> Are Responsible for Erythromycin Resistance in <i>Riemerella anatipestifer</i>

<div><p>To investigate the genetic basis of erythromycin resistance in <i>Riemerella anatipestifer</i>, the MIC to erythromycin of 79 <i>R</i>. <i>anatipestifer</i> isolates from China and one typed strain, ATCC11845, were evaluated. The results showed that 43 of 80 (53.8%) of the tested <i>R</i>. <i>anatipestifer</i> strains showed resistance to erythromycin, and 30 of 43 erythromycin-resistant <i>R</i>. <i>anatipestifer</i> strains carried <i>ermF</i> or <i>ermFU</i> with an MIC in the range of 32–2048 μg/ml, while the other 13 strains carrying the <i>ereD</i> gene exhibited an MIC of 4–16 μg/ml. Of 30 <i>ermF</i> + <i>R</i>. <i>anatipestifer</i> strains, 27 (90.0%) carried the <i>ermFU</i> gene which may have been derived from the CTnDOT-like element, while three other strains carried <i>ermF</i> from transposon Tn4351. Moreover, sequence analysis revealed that <i>ermF</i>, <i>ermFU</i>, and <i>ereD</i> were located within the multiresistance region of the <i>R</i>. <i>anatipestifer</i> genome.</p></div

Distribution of <i>R</i>. <i>anatipestifer</i> strains with different MICs for erythromycin.

<p>Distribution of <i>R</i>. <i>anatipestifer</i> strains with different MICs for erythromycin.</p

Air Stable O3-Type Cathode Material with Nanometer Size for Durable Low-Temperature Sodium-Ion Batteries

The application of sustainable sodium-ion batteries (SIBs) in large-scale electrical grids is primarily hindered by the bad cycling lifetime and cryogenic performance. To address these issues, we propose an O3-NaNi0.455Cu0.05Mn0.485Sb0.01O2 (NaNCMS) cathode material via Cu and Sb codoping. This NaNCMS material can preserve pristine structural and electrochemical properties when suffering from high humidity. The bulk-like shapes with nanoscale and balanced Na+ diffusion kinetics enable the NaNCMS cathode to have a high reversible capacity (134 mAh g–1 at 0.2C) and improved rate capability at room temperature. The monoclinic O′3 phase, which is responsible for huge structure reconstruction and volume change in pristine NaNi0.5Mn0.5O2, is inhibited by Cu–Sb substitution. Hence, a better cycling stability is presented for the NaNCMS cathode. Moreover, excellent electrochemical properties are exhibited at −15 °C. The low-temperature battery based on the NaNCMS cathode holds high reversible capacities of 126, 119, and 112 mAh g–1 at 0.2 0.5, and 1C, respectively. It also demonstrates a capacity retention of 72% with a final capacity of 81 mAh g–1 after 400 cycles (1C) and 62% after 1000 cycles (3C) at −15 °C