60 research outputs found
Combination of cyclooxygenase-2 inhibitor and doxorubicin increases the growth inhibition and apoptosis in human hepatocellular carcinoma cells
Inhibition of cyclooxygenase (COX)-2 elicits therapeutic effects in solid tumors that are coupled with the inhibition of cell proliferation and induction of apoptosis in tumor cells. Aim: This study was designed to investigate the role of COX-2 inhibitor nimesulide in cell growth and apoptosis of the cultured human hepatocellular carcinoma HepG2 cells. Methods: We performed the MTT assay, flow cytometric analysis and cell morphology study to evaluate growth inhibition and cell apoptosis upon the action of nimesulide alone or along with doxorubicin, a common agent for the treatment of human hepatocellular carcinoma. Results: Our results showed that the treatment of HepG2 cells with more than 50 Β΅M of nimesulide suppressed COX-2 enzyme activity because of reduced PGE2 production, and then induced growth inhibition and cell apoptosis despite no alterations of COX-2 protein expression. Importantly, the combination of 50 Β΅M or 100 Β΅M of nimesulide and low concentrations (5 Β΅M to 20 Β΅M) of doxorubicin resulted in enhanced cell growth inhibition, apoptosis induction and reduced VEGF production. Conclusion: These data suggest synergistic and/or additive effects of COX-2 inhibitors and chemotherapeutic agents, and may provide the rational for clinical studies of COX-2 inhibitors on the treatment or chemoprevention of human hepatocellular carcinoma.Π£Π³Π½Π΅ΡΠ΅Π½ΠΈΠ΅ ΡΠΈΠΊΠ»ΠΎΠΎΠΊΡΠΈΠ³Π΅Π½Π°Π·Ρ-2 (Π¦ΠΠ-2) ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅Ρ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ ΠΏΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΠΎΠ»ΠΈΠ΄Π½ΡΠΌΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΡΠΌΠΈ
ΠΈ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΠΈΠ½Π΄ΡΠΊΡΠΈΠ΅ΠΉ Π°ΠΏΠΎΠΏΡΠΎΠ·Π°. Π¦Π΅Π»Ρ: ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ»ΠΈ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ°
Π¦ΠΠ-2 β Π½ΠΈΠΌΠ΅ΡΡΠ»ΠΈΠ΄Π° Π² ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ
ΡΠΎΡΡΠ° ΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π³Π΅ΠΏΠ°ΡΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° HepG2.
ΠΠ΅ΡΠΎΠ΄Ρ: Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΈ ΡΠ³Π½Π΅ΡΠ΅Π½ΠΈΡ ΡΠΎΡΡΠ° ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ Π½ΠΈΠΌΠ΅ΡΡΠ»ΠΈΠ΄Π° ΡΠ°ΠΌΠΎΡΡΠΎΡΡΠ΅Π»ΡΠ½ΠΎ ΠΈ Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ Ρ
Π΄ΠΎΠΊΡΠΎΡΡΠ±ΠΈΡΠΈΠ½ΠΎΠΌ ΠΏΡΠΈΠΌΠ΅Π½ΡΠ»ΠΈ MTT-Π°Π½Π°Π»ΠΈΠ·, ΠΏΡΠΎΡΠΎΡΠ½ΡΡ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΡ ΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΠ΅ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ:
ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΊΠ»Π΅ΡΠΎΠΊ HepG2 cells Π½ΠΈΠΌΠ΅ΡΡΠ»ΠΈΠ΄ΠΎΠΌ Π² ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ > 50 ΞΌM ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΊ ΡΠ³Π½Π΅ΡΠ΅Π½ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ
Π¦ΠΠ-2 Π·Π° ΡΡΠ΅Ρ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ PGE2
, ΠΏΠΎΡΠ»Π΅ ΡΠ΅Π³ΠΎ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠΎΡΡΠ° ΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ· ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΡΠΈ Π½Π΅ΠΈΠ·ΠΌΠ΅Π½Π΅Π½Π½ΠΎΠΌ
ΡΡΠΎΠ²Π½Π΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π¦ΠΠ-2. ΠΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ 50 ΞΌM ΠΈΠ»ΠΈ 100 ΞΌM Π½ΠΈΠΌΠ΅ΡΡΠ»ΠΈΠ΄Π° ΠΈ Π΄ΠΎΠΊΡΠΎΡΡΠ±ΠΈΡΠΈΠ½Π° Π² ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ
5β20 ΞΌM ΠΎΠ±ΡΡΠ»ΠΎΠ²ΠΈΠ»ΠΎ ΡΡΠΈΠ»Π΅Π½Π½ΠΎΠ΅ ΡΠ³Π½Π΅ΡΠ΅Π½ΠΈΠ΅ ΡΠΎΡΡΠ° ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ VEGF. ΠΡΠ²ΠΎΠ΄Ρ:
ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΈΠ½Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΈ/ΠΈΠ»ΠΈ Π°Π΄Π΄ΠΈΡΠΈΠ²Π½ΠΎΠΌ ΡΡΡΠ΅ΠΊΡΠ΅ ΠΏΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠΎΠ² Π¦ΠΠ-2
ΠΈ Ρ
ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ²
The Periodic Instability of Diameter of ZnO Nanowires via a Self-oscillatory Mechanism
ZnO nanowires with a periodic instability of diameter were successfully prepared by a thermal physical vapor deposition method. The morphology of ZnO nanowires was investigated by SEM. SEM shows ZnO possess periodic bead-like structure. The instability only appears when the diameter of ZnO nanowires is small. The kinetics and mechanism of Instability was discussed at length. The appearance of the instability is due to negative feed-back mechanism under certain experimental conditions (crystallization temperature, vapor supersaturation, etc)
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