[2-({Benz­yl[2-(benz­yl{5-methyl-2-oxido-3-[(pyridin-2-ylmeth­yl)imino­meth­yl]benz­yl}amino)­eth­yl]aza­nium­yl}meth­yl)-4-methyl-6-[(pyridin-2-ylmeth­yl)imino­meth­yl]phenolato]nickel(II) perchlorate methanol disolvate

In the solvated title complex, [Ni(C46H47N6O2)]ClO4·2CH4O, the coordination sphere around the NiII ion can be described as distorted cis-NiO2N4 octa­hedral defined by two phenolate O atoms and four N atoms from the hexa­dentate ligand. An intra­molecular bifurcated N—H⋯(N,O) hydrogen bond helps to establish the conformation of the complex mol­ecule. In the crystal, the components are connected by O—H⋯O and C—H⋯O hydrogen bonds

Inhibition of hepatocelluar carcinoma MAT2A and MAT2beta gene expressions by single and dual small interfering RNA

RNA interference (RNAi) has been successfully applied in suppression of hepatic cancer genes. In hepatocelluar carcinoma cell, one methionine adenosyltransferase (MAT) isozyme, MATII was found to have two catalytic subunits which were encoded by MAT2A and MAT2β respectively. During tumorigeness of hepatocelluar carcinoma, expressions of the two genes were discovered to be increased combining with a switch of MAT (form MATI to MATII), To figure out the role played by MATII in hepatic cancer, In this study, for the first time we established a dual small interfering RNA (siRNA) expression system, which could simultaneously express two different siRNA molecules specifically targeting two genes. To test the effectiveness of this system, we applied this approach to express simultaneously two different siRNA duplexes that specifically target MAT2A and MAT2β genes of hepatocelluar carcinoma respectively in HepG2 cell. Results indicated that dual siRNA could simultaneously inhibit the expression of MAT2A and MAT2β gene by 89.5% and 97.8% respectively, In addition, dual siRNA molecules were able to significantly suppress growth of hepatocelluar carcinoma cell in vitro as well as induce apoptosis which was involved in arrest cell cycle at the G1/S checkpoint and the expressions of p21, p27 and Bax

Bis[N-(2-furylmeth­yl)ethane-1,2-di­amine]­bis­(perchlorato)copper(II)

In the title complex, [Cu(ClO4)2(C7H12N2O)2], the Cu(II) ion lies on a crystallographic inversion centre. The coordination sphere around Cu(II) ion can be described as tetragonally distorted octa­hedral with two perchlorate O atoms occupying the apical positions and four N atoms from two N 1-(2-furyl­methyl)ethane-1,2-diamine ligands in the basal plane

[μ-10,22-Dichloro-3,6-bis­(2-furylmeth­yl)-3,6,14,18-tetra­azatricyclo­[18.3.1.18,12]penta­cosa-1(23),8,10,12(25),13,18,20(24),21-octa­ene-24,25-diolato-κ8 N 3,N 6,O 24,O 25:N 14,N 18,O 24:O 25]bis­[chloridocopper(II)] acetonitrile solvate

The title compound, [Cu2(C31H30Cl2N4O4)Cl2]·CH3CN, was synthesized by cyclo­condensation between N,N′-bis­(2-fur­yl)-N,N′-bis­(3-formyl-5-chloro­salicylaldehyde)ethyl­enediamine and 1,3-diamino­propane in the presence of CuII ions. It is an unsymmetrical dinuclear CuII complex. The coordination geometry for each CuII atom can be discribed as distorted square-pyramidal. The two Cu atoms are bridged by two phenolate O atoms with a Cu⋯Cu distance of 3.0274 (9) Å

[μ-10,21-Dimethyl-3,6,14,17-tetra­za­tricyclo­[17.3.1.18,12]tetra­cosa-1(23),2,6,8,10,12 (24),13,17,19,21-deca­ene-23,24-diolato-κ4 N 3,N 6,O 23,O 24:κ4 N 14,N 17,O 23,O 24]bis­(perchlorato-κO)dimanganese(II)

In the centrosymmetric and dinuclear title complex, [Mn2(C22H22N4O2)(ClO4)2], the two Mn atoms are bridged by two phenolate O atoms of the N4O2 macrocycle with an Mn⋯Mn distance of 2.9228 (11) Å. The distorted square–pyramidal N2O3 coordination geometry is completed by an O atom derived from a perchlorate anion

[μ-11,23-Dibromo-3,7,15,19-tetra­aza­tri­cyclo­[19.3.1.19,13]hexa­cosa-1(25),2,7,9,11,13(26),14,19,21,23-deca­ene-25,26-diolato-κ4 N 3,N 7,O,O′:κ4 O,O′,N 15,N 19]bis[perchloratocopper(II)]

The title complex, [Cu2(C22H20Br2N4O2)(ClO4)2], was prepared by the condensation of 2,6-diformyl-4-bromo­phenol with 1,3-diamino­propane in the presence of copper(II) ions. The macrocyclic ligand shows an approximately planar structure except for the two propene groups in the macrocycle. The coordination polyhedron of each Cu atom can be described as distorted square pyramidal. The two Cu atoms are bridged by two phenolate O atoms of the macrocycle, with a Cu⋯Cu distance of 3.109 (2) Å

Bis(μ-4-fluoro-2,6-diformyl­phenolato)bis­[diaqua­nickel(II)] dichloride

In the title dinuclear nickel(II) complex, [Ni2(C8H4FO3)2(H2O)4]Cl2, synthesized by the reaction between 4-fluoro-2,6-diformyl­phenol and nickel(II) chloride in methanol, the coordination cation is located on an inversion center and the NiII atom adopts a slightly distorted octa­hedral coordination geometry. The two Ni atoms are bridged by two phenolate O atoms and the intra­molecular Ni⋯Ni distance is 3.0751 (9) Å. The crystal structure is stabilized by O—H⋯Cl hydrogen bonds