High Throughput Analysis of Photocatalytic Water Purification

We present a novel high throughput photocatalyst efficiency assessment method based on 96-well microplates and UV–vis spectroscopy. We demonstrate the reproducibility of the method using methyl orange (MO) decomposition and compare kinetic data obtained with those provided in the literature for larger conventional photoreactors. To demonstrate the capabilities of the method, we rapidly screened the effects of salts, potentially present in wastewater, on kinetic rates of MO decomposition and briefly discuss the obtained data on the basis of existing literature

Histological evaluation of tissue formation in the three ceramics upon intramuscular implantation in rats, rabbits and dogs.

<p>Representative images showing tissue response to BCP1100 (A, D, G), BCP1200 (B, E, H) and HA1200 (C, F, I) upon implantation in the femoral muscle of rat, dorsal muscle of rabbit and dog for 6 weeks. Decalcified sections, HE Staining, magnification = ×100, scale bar = 200 µm; V→ Blood vessel, CT→Connective tissue, M→Material, O→Osteoid tissue, B→Bone.</p

Histological evaluation of bone formation upon implantation of the three ceramics in dorsal muscles of dogs for 12 weeks.

<p>The highest amount of osteoid and ectopic bone were observed in BCP1100 (A), followed by BCP1200 (B) an HA1200 (C). In all cases, bone was trabecular in appearance, with laminar bone and osteocytes contained in the lacunae, and infiltrated by blood vessels. Decalcified sections, Masson’s trichrome staining, magnification = ×100, scale bar = 200 µm; V→Blood vessel, CT→Connective tissue, M→Material, O→Osteoid tissue, B→Bone.</p

SEM micrographs showing macrostructure (A–C) and surface microstructure (D–F) of BCP1100 (A,D), BCP1200 (B,E) and HA1200 (C,F).

<p>All ceramics were porous with similar macroporous structure (A–C). The surface of the BCP1100 (D) ceramic exhibited smaller grains and a larger number of micropores than the chemically identical BCP1200, that was sintered at 1200°C (E). Grain size and number of micropores in HA1200 were between those of BCP110 and BCP1200 (F), which is in accordance with specific weight measurements in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107044#pone-0107044-t001" target="_blank">Table 1</a>. Scale bar = 1 mm for A–C and 10 µm for D–F.</p

A summary of tissue response to the three types of ceramics in different animals.

<p>A summary of tissue response to the three types of ceramics in different animals.</p

XRD patterns of the three ceramics.

<p>The patterns of BCP1100 and BCP1200 demonstrated biphasic nature of the ceramics consisting of HA and β-TCP. No apparent differences were found as a result of difference in sintering temperature. The pattern of HA1200 was typical of phase-pure hydroxyapatite.</p

Physico-chemical characterization of biphasic calcium phosphate sintered at 1100°C (BCP1100), of biphasic calcium phosphate sintered at 1200°C (BCP1200) and of phase-pure hydroxyapatite sintered at 1200°C (HA1200).

<p>Physico-chemical characterization of biphasic calcium phosphate sintered at 1100°C (BCP1100), of biphasic calcium phosphate sintered at 1200°C (BCP1200) and of phase-pure hydroxyapatite sintered at 1200°C (HA1200).</p

sj-docx-1-tej-10.1177_20417314221149208 – Supplemental material for Hypoxia-tolerant apical-out intestinal organoids to model host-microbiome interactions

Supplemental material, sj-docx-1-tej-10.1177_20417314221149208 for Hypoxia-tolerant apical-out intestinal organoids to model host-microbiome interactions by Panagiota Kakni, Barry Jutten, Daniel Teixeira Oliveira Carvalho, John Penders, Roman Truckenmüller, Pamela Habibovic and Stefan Giselbrecht in Journal of Tissue Engineering</p

Primer sequences of the osteogenic genes, the expression of which was investigated using qPCR analysis.

<p>Primer sequences of the osteogenic genes, the expression of which was investigated using qPCR analysis.</p

ALP activity, normalized to DNA amount of MG-63 cells cultured on 2/6, 4/11 and flat surface in basic or osteogenic medium for up to 21 days.

<p>The effect of topography on the ALP activity was mild, with cells cultured in OM on 4/11 showing a significantly lower activity as compared to the flat control at 14 days, and to both the flat control and 2/6 at 21 days. Statistically significant differences are marked with * for <i>p</i> < 0.05 and **for <i>p</i> < 0.01.</p