Usnic acid, a secondary metabolite of lichens and its effect on in vitro digestibility in reindeer

Usnic acid, a common secondary metabolite in prefered lichens by reindeer and caribou, has been tested for its effect on In Vitro Dry Matter Digestibility (IVDMD) using inocula from four reindeer. When Cladonia alpestris (stellaris (OpicJ) was used as substrate and reindeer rumen liqour as media of incubation together with usnic acid, digestibility was considerably enhanced. This was also true for a lower prefered lichen Stereocaulon paschale (L.), but the effect was less pronounced. The results suggest that reindeer host some rumen microorganism able to metabolize lichen secondary metabolities

DISTRIBUTION OF WINTER BROWSING BY MOOSE: EVIDENCE OF LONG-TERM STABILITY IN NORTHERN SWEDEN

Predicting spatial distribution of large herbivore foraging is important for successful management, but accurate predictions remain elusive against a background of multiple causes modified by environmental stochasticity. Moose (Alces alces) might prefer to browse areas with high plant density, but if snow depth co-varies with plant density, this could restrict access to these sites and force use of sites with lower plant density and snow depth. Moose browsing was measured in 72 plots distributed within the subarctic birch (Betula spp.) forest landscape at Abisko in northern Sweden in 1996. In 2010, the same plots were revisited and the measurements repeated. A generalized linear model predicted moose browsing on birch in 2010 from the browsing pattern on birch measured in 1996. The model suggested that neither total density of willow and birch stems nor snow depth were influential of foraging distribution of birch at multiple spatial scales. The spatial scale at which clustering of browsing on birch occurred, coincided with the scale of clustering of birch and willow (Salix spp.) stems at distances of 1000-2500 m; at lesser distance browsing was distributed randomly. We concluded that moose demonstrate stability in spatial browsing patterns after 14 years which corresponds to 3-4 generations of moose, and that plant density represents a cue for moose only at certain scales. Predictability of feeding sites is valuable for long-term moose and forest management, and conservation planning

SEASONAL VARIATION OF PHENOLS, NITROGEN, FIBER, AND IN VITRO DIGESTIBILITY IN SWEDISH MOOSE

Understanding how different components of food are processed and digested within the compartments of the digestive tract of large herbivores has important implications in their foraging behaviour, nutritional ecology, and techniques for measuring diet composition and nutritional quality of forage. Analysis of contents from different compartments of the digestive tract of moose in central Sweden showed that neutral detergent fiber (NDF) and nitrogen (N) content varied throughout the digestive tract and among individual moose (Alces alces). Total phenols (TP) had an inconsistent pattern throughout the digestive tract, possibly reflecting variation in diet composition and phenol patterns. The study moose were divided into 2 groups; the winter group had low N in the digestive tract and high NDF and dry matter content, and the summer group had high levels of N and low NDF and dry matter content. The phenol platyphyllane, indicative of consumption of dormant silver birch (Betula pendula), was detected throughout the contents of the digestive tract in 2 animals in the winter group. The winter moose had higher NDF than summer moose, indicating the seasonal change in diet quality. In vitro organic matter digestibility (IVOMD) was not different between the summer and winter diets. The effect of birch phenols on IVOMD was concentration-dependent; differences between seasons were apparent at only the highest concentration. The 2 groups had marked differences in digestive content of major nutrients, NDF, and ability to digest forage which were consistent with typical variation in seasonal diet quality

SEASONAL VARIATION OF PHENOLS, NITROGEN, FIBER, AND IN VITRO DIGESTIBILITY IN SWEDISH MOOSE

Understanding how different components of food are processed and digested within the compartments of the digestive tract of large herbivores has important implications in their foraging behaviour, nutritional ecology, and techniques for measuring diet composition and nutritional quality of forage. Analysis of contents from different compartments of the digestive tract of moose in central Sweden showed that neutral detergent fiber (NDF) and nitrogen (N) content varied throughout the digestive tract and among individual moose (Alces alces). Total phenols (TP) had an inconsistent pattern throughout the digestive tract, possibly reflecting variation in diet composition and phenol patterns. The study moose were divided into 2 groups; the winter group had low N in the digestive tract and high NDF and dry matter content, and the summer group had high levels of N and low NDF and dry matter content. The phenol platyphyllane, indicative of consumption of dormant silver birch (Betula pendula), was detected throughout the contents of the digestive tract in 2 animals in the winter group. The winter moose had higher NDF than summer moose, indicating the seasonal change in diet quality. In vitro organic matter digestibility (IVOMD) was not different between the summer and winter diets. The effect of birch phenols on IVOMD was concentration-dependent; differences between seasons were apparent at only the highest concentration. The 2 groups had marked differences in digestive content of major nutrients, NDF, and ability to digest forage which were consistent with typical variation in seasonal diet quality

The giant, horizontal and asymptotic branches of galactic globular clusters. I. The catalog, photometric observables and features

A catalog including a set of the most recent Color Magnitude Diagrams (CMDs) is presented for a sample of 61 Galactic Globular Clusters (GGCs). We used this data-base to perform an homogeneous systematic analysis of the evolved sequences (namely, Red Giant Branch (RGB), Horizontal Branch (HB) and Asymptotic Giant Branch (AGB)). Based on this analysis, we present: (1) a new procedure to measure the level of the ZAHB (V_ZAHB) and an homogeneous set of distance moduli obtained adopting the HB as standard candle; (2) an independent estimate for RGB metallicity indicators and new calibrations of these parameters in terms of both spectroscopic ([Fe/H]_CG97) and global metallicity ([M/H], including also the alpha-elements enhancement). The set of equations presented can be used to simultaneously derive a photometric estimate of the metal abundance and the reddening from the morphology and the location of the RGB in the (V,B-V)-CMD. (3) the location of the RGB-Bump (in 47 GGCs) and the AGB-Bump (in 9 GGCs). The dependence of these features on the metallicity is discussed. We find that by using the latest theoretical models and the new metallicity scales the earlier discrepancy between theory and observations (~0.4 mag) completely disappears.Comment: 51 pages, 23 figures, AAS Latex, macro rtrpp4.sty included, accepted by A

Demographic Factors Associated with Hantavirus Infection in Bank Voles (Clethrionomys glareolus)

The bank vole (Clethrionomys glareolus) is the natural reservoir of Puumala virus (PUUV), a species in the genus Hantavirus. PUUV is the etiologic agent of nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. Factors that influence hantavirus transmission within host populations are not well understood. We evaluated a number of factors influencing on the association of increased PUUV infection in bank voles captured in a region in northern Sweden endemic for the virus. Logistic regression showed four factors that together correctly predicted 80% of the model outcome: age, body mass index, population phase during sampling (increase, peak, or decline/low), and gender. This analysis highlights the importance of population demography in the successful circulation of hantavirus. The chance of infection was greatest during the peak of the population cycle, implying that the likelihood of exposure to hantavirus increases with increasing population density

Human Hantavirus Infections, Sweden

The prevalent human hantavirus disease in Sweden is nephropathia epidemica, which is caused by Puumala virus and shed by infected bank voles (Clethrionomys glareolus). To evaluate temporal and spatial patterns of this disease, we studied 2,468 reported cases from a highly disease-endemic region in northern Sweden. We found that, in particular, middle-aged men living in rural dwellings near coastal areas were overrepresented. The case-patients were most often infected in late autumn, when engaged in activities near or within manmade rodent refuges. Of 862 case-patients confident about the site of virus exposure, 50% were concentrated within 5% of the study area. The incidence of nephropathia epidemica was significantly correlated with bank vole numbers within monitored rodent populations in part of the region. Understanding this relationship may help forestall future human hantavirus outbreaks

Cancer survival in the elderly: Effects of socio-economic factors and health care system features (ELDCARE project)

The purpose of the ELDCARE project is to study differences in cancer survival for elderly patients by country, taking into account the socio-economic conditions and the characteristics of health care systems at the ecological level. Fifty-three European cancer registries, from 19 countries, participating in the EUROCARE 3 programme, collected information to compute relative survival on patients aged 65-84 years, diagnosed over the period 1990-1994. National statistics offices provided the macro-economic and labour force indicators (gross domestic product, total health expenditure, and proportion of people employed in the agriculture sector) as well as the features of national health care systems. Survival for several of the cancer sites had high positive Pearson's correlations (r) with the affluence indicators (usually r > 0.7), but survival for the poor prognosis cancers (lung, ovary, stomach) and for cervix uteri was not so well correlated. Among the medical resources considered, the number of computed tomography scanners was the variable most related to survival in the elderly; the number of total health practitioners in the country did not show any relationship. Survival was related to the marital status of elderly women more strongly than for men and younger people. The highest correlations of survival with the percentage of married elderly women in the population were for cancers of the rectum (r = 0.79) and breast (r = 0.66), while survival correlated negatively with the proportion of widows for most cancers. Being married or widowed is for elderly people, in particular elderly women, an important factor influencing psychological status, life habits and social relationships. Social conditions could play a major role in determining health outcomes, particularly in the elderly, by affecting access to health care and delay in diagnosis

Challenges and opportunities in mixed method data collection on mental health issues of health care workers during COVID-19 pandemic in India

Background: The present paper describes the key challenges and opportunities of mixed method telephonic data collection for mental health research using field notes and the experiences of the investigators in a multicenter study in ten sites of India. The study was conducted in public and private hospitals to understand the mental health status, social stigma and coping strategies of different healthcare personnel during the COVID-19 pandemic in India.Methods: Qualitative and quantitative interviews were conducted telephonically. The experiences of data collection were noted as a field notes/diary by the data collectors and principal investigators.Results: The interviewers reported challenges such as network issues, lack of transfer of visual cues and sensitive content of data. Although the telephonic interviews present various challenges in mixed method data collection, it can be used as an alternative to face-to-face data collection using available technology.Conclusions: It is important that the investigators are well trained keeping these challenges in mind so that their capacity is built to deal with these challenges and good quality data is obtained

UHPLC-ESI/TOFMS Determination of Salicylate-like Phenolic Gycosides in Populus tremula Leaves

Associations of salicylate-like phenolic glycosides (PGs) with biological activity have been reported in Salix and Populus trees, but only for a few compounds, and in relation to a limited number of herbivores. By considering the full diversity of PGs, we may improve our ability to recognize genotypes or chemotype groups and enhance our understanding of their ecological function. Here, we present a fast and efficient general method for salicylate determination in leaves of Eurasian aspen that uses ultra-high performance liquid chromatography-electrospray ionization/time-of-flight mass spectrometry (UHPLC-ESI/TOFMS). The time required for the liquid chromatography separations was 13.5 min per sample, compared to around 60 min per sample for most HPLC protocols. In leaf samples from identical P. tremula genotypes with diverse propagation and treatment histories, we identified nine PGs. We found the compound-specific mass chromatograms to be more informative than the UV-visible chromatograms for compound identification and when quantitating samples with large variability in PG content. Signature compounds previously reported for P. tremoloides (tremulacin, tremuloidin, salicin, and salicortin) always were present, and five PGs (2'-O-cinnamoyl-salicortin, 2'-O-acetyl-salicortin, 2'-O-acetyl-salicin, acetyl-tremulacin, and salicyloyl-salicin) were detected for the first time in P. tremula. By using information about the formic acid adduct that appeared for PGs in the LTQ-Orbitrap MS environment, novel compounds like acetyl-tremulacin could be tentatively identified without the use of standards. The novel PGs were consistently either present in genotypes regardless of propagation and damage treatment or were not detectable. In some genotypes, concentrations of 2'-O-acetyl-salicortin and 2'-O-cinnamoyl-salicortin were similar to levels of biologically active PGs in other Salicaceous trees. Our study suggests that we may expect a wide variation in PG content in aspen populations which is of interest both for studies of interactions with herbivores and for mapping population structure