8 research outputs found
Analisi trascrizionale di geni coinvolti nella risposta agli stress abiotici in pioppo (Populus spp.)
Response of rumen microbial ecosystem to diets integrated with chestnut or quebracho tannins in dairy ewes
The aim of this study was to evaluate the response of rumen microbial ecosystem to diets integrated with chestnut or quebracho
tannins in dairy ewes. The experiment was conducted as 3 X 3 Latin square design and the trial was repeated 2 times. Three
fistulated ewes fed 3 diets based on chopped grass hay ad libitum administered and on 800 g / head and day of 3 experimental
concentrates containing 84.5 g of soybean oil / kg of DM and 52.8 g / kg DM of bentonite (CON) or chestnut tannin extract (CHT)
or quebracho tannin extract (QUE). At the end of each experimental period rumen liquor was analysed for fatty acid and microbial
profiles. On the basis of the molar stoichiometric relations between rumen volatile fatty acid and CH4 production (CH4=0.45 x
acetate-0.275 x propionate + 0.4 x butyrate), the CH4 emission was also predicted for each diet. A canonical correspondence
analysis (CCA) was performed in order to find potential connection between microbial community, fatty acid composition of rumen
liquor and potential CH4 emission, and how these connections are influenced by the different diets. DGGE bands were used as
"species" data, while fatty acids and potential CH4 emission as "environmental" variables.
The microbial profile was affected by the presence of tannins. The bacterial community of QUE and CHT samples of rumen liquor
was positively correlated to vaccenic acid, conjugated linoleic acid and C18:2 trans-11 cis-15. Moreover, the bacterial
communities as affected by CHT resulted mainly positively correlated to butyric acid, acetic acid and with potential CH4 emission.
In contrast, the bacterial communities as affected by CON resulted mainly correlated positively to C18:2 cis-9 cis-12 and C18:0
production
Milk fatty acid composition, rumen microbial population and animal performances in response to diets rich in linoleic acid supplemented with chestnut or quebracho tannins in dairy ewes.
Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes
Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. e aim of this study was to evaluate the e ect of dietary supplementation with chestnut and quebracho tannins on microbial community and fatty acid pro le, in the rumen uid of dairy ewes. Multivariate analysis of PCR-DGGE pro les of rumen microbial communities showed a correlation among the presence of chestnut or quebracho in the diet, the speci c Butyrivibrio group DGGE pro les, the increase in 18:3 cis9, cis12, and cis15; 18:2 cis9 and cis12; 18:2 cis9 and trans11; 18:2 trans11 and cis15; and 18:1 trans11 content, and the decrease in 18:0 concentration. Phylogenetic analysis of DGGE band sequences revealed the presence of bacteria representatives related to the genera Hungatella, Ruminococcus, and Eubacterium and unclassi ed Lachnospiraceae family members, suggesting that these taxa could be a ected by tannins presence in the diets. e results of this study showed that tannins from chestnut and quebracho can reduce the biohydrogenation of unsaturated fatty acids through changes in rumen microbial communities
Effect of water deficit on expression of stress-related genes in the cambial region of two contrasting poplar clones
Effect of stoned olive pomace on rumen microbial communities and polyunsaturated fatty acid biohydrogenation: an in vitrostudy
Expanded access program with Nusinersen in SMA type I in Italy: Strengths and pitfalls of a successful experience
Expanded access program with Nusinersen in SMA type
An observational study of functional abilities in infants, children, and adults with type 1 SMA
ObjectiveTo report cross-sectional clinical findings in a large cohort of patients affected by type 1 spinal muscular atrophy.MethodsWe included 122 patients, of age ranging between 3 months and 22 years, 1 month. More than 70% (85/122) were older than 2 years and 25% (31/122) older than 10 years. Patients were classified according to the severity of phenotype and to the number of SMN2 copies.ResultsPatients with the more common and the most severe phenotype older than 2 years were, with few exceptions, on noninvasive ventilation and, with increasing age, more often had tracheostomy or >16-hour ventilation and a gastrostomy inserted. In contrast, 25 of the 28 patients with the mildest phenotype older than 2 years had no need for tracheostomy or other ventilatory or nutritional support. In patients older than 2 years, the Children's Hospital of Philadelphia Infant Test of Neuromuscular Disorders scores were generally lower compared to those found in younger patients and showed distinct levels of functional abilities according to the severity of the phenotype. Similar findings were also observed on the Hammersmith Infant Neurological Examination.ConclusionsOur findings confirm that, after the age of 2 years, patients with type 1 spinal muscular atrophy generally survive only if they have gastrostomy and tracheostomy or noninvasive ventilation >16 hours and have low scores on the functional scales. More variability, however, can be expected in those with the mildest phenotype, who achieve head control. These data provide important baseline information at the time treatments are becoming available