Comparison of measurements of canine plasma glucose, creatinine, urea, total proteins, alanine aminotransferase, and alkaline phosphatase obtained with the APOLOWAKO and Vitros 250 analyzers

The APOLOWAKO is an entirely automatic benchtop biochemistry analyzer that uses stabilized liquid reagents. It was tested for canine blood and plasma glucose, creatinine, urea, total proteins, alanine aminotransferase, and alkaline phosphatase. The APOLOWAKO gave very similar results for whole blood and the corresponding plasma (n = 32). Within-laboratory imprecision was below 2.2% and 5.8% for substrates and enzymes, respectively. Comparison of results with whole blood by APOLOWAKO and with the corresponding plasma by Vitros 250 (n = 139) showed very good correlations. Passing–Bablok’s regression slopes ranged from 0.83 to 1.12 and intercepts were close to zero, except for ALP where the results obtained by APOLOWAKO were approximately 1.5 times higher than by Vitros. The APOLOWAKO system can be a reliable instrument in veterinary practices where larger systems are not available but it should be further validated and reference intervals should be determined

Apoptose et cancer (bcl2, p53)

National audienc

Glomerulus from the azotemic Dogue de Bordeaux dog.

<p>Global consolidation of capillary lumens by extracellular matrix, synechiae and deposition of hyaline material (arrow) are visible. Parietal epithelium and podocytes are hypertrophied. Periodic acid—Schiff staining (400x).</p

Correlations (r) between urinary markers, UPC and storage duration.

<p>UPC, urine protein-to-creatinine ratio; Storage, storage duration; uALB/c, urinary albumin-to-creatinine ratio; uRBP/c, urinary retinol binding protein-to-creatinine ratio; uNAG/c, urinary N-acetyl-β-D-glucosaminidase-to-creatinine ratio.</p><p>* Significant correlation p<0.05</p><p>** Significant correlation p<0.0005</p><p>Correlations (r) between urinary markers, UPC and storage duration.</p

Example of the different results for SDS-AGE performed on urine.

<p>Lane 1 represents the migration of molecular markers, lane 2 a normal pattern, lane 3 a tubular pattern, lane 4 an abnormal mixed pattern, lane 5 an abnormal glomerular pattern from the non azotemic dog with confirmed familial GN. Lanes 6 and 7 both represent glomerular pattern from dogs with stage II and III CKD respectively. The various protein bands identified are indicated by arrowheads. The numbers on the left side correspond to molecular weight of molecular markers in kDa.</p

Glomerular capillary from the non azotemic Dogue de Bordeaux dog.

<p>Capillary lumen is open, and podocyte foot processes are segmentally effaced (arrows). Transmission electron microscopy.</p

Glomerulus from the non azotemic Dogue de Bordeaux dog.

<p>Mild global increase of mesangial matrix, mild increase of Bowman’s space and thickening of Bowman’s capsule (arrow) are visible. Periodic acid—Schiff staining (400x).</p