1 research outputs found
Plasmid mediated resistance in multidrug resistant bacteria isolated from children with suspected septicaemia in Zaria, Nigeria
Septicaemia is a common cause of morbidity and mortality among children in theĀ developing world. The knowledge of theĀ epidemiological and antimicrobial pattern of common pathogens that cause septicaemia is useful for prompt treatment of patients. Fifty-five (55) clinical isolates from children with suspected septicaemia were used for the study. The isolates include Coagulase negative Staphylococcus, Staphylococcus aureus, Salmonella spp., Klebsiella spp., Citrobacter spp., Proteus spp andĀ Pseudomonas spp. The antibiotic susceptibility testing of isolated bacteria associated with septicaemia in children were carried out using standardĀ microbiological protocol. The MAR index for the test bacterial isolates wasĀ determined and the bacterial isolates that displayed multiple antibiotic resistance were investigated for the presence of resistant factor such as plasmids. The sizes of the plasmid observed in the bacterial isolates were determined using agarose gel electrophoresis. Observations made from the agarose gel electrophoresis showed that majority of the multiple antibiotic resistant isolates haboured plasmids DNA of different sizes viz: 10.00 Kb, 8.71 Kb, 7.08 Kb, 1.02 Kb, 1.00 Kb, 0. 98 Kb and 0.87 Kb. The plasmid analysis of the results obtained in this study showed that theĀ predominant plasmid molecular size was 977bp which occurred frequently amongĀ the Citrobacter spp and Staph aureus. These findings suggest an increasedĀ resistance to the antibiotics commonly used for the treatment of septicaemia, and the observed presence of plasmids in some of the test bacteria isolated shows that they could have been acquired from multiple antibiotic resistant bacteria in the community under investigation.Key words: Children, Multiple antibiotic resistance, Plasmids, Septicaemi