GROWTH INHIBITION AND DIFFERENTIATION OF HUMAN BREAST CANCER CELLS BY THE PAFR ANTAGONIST WEB-2086

WEB-2086 – an antagonist of platelet-activating factor receptor (PAFR) with known anti-inflammatory, antiangiogenic and antileukaemic properties – also proved to inhibit the proliferation in human solid tumour cell lines of different histology, and with much higher efficacy than in normal fibroblasts. A detailed analysis of WEB-2086 anticancer activity was then performed focusing on breast adenocarcinoma MCF-7 and MDA-MB-231 cells. WEB-2086-treated cells, either expressing (MCF-7) or unexpressing (MDA-MB-231) the oestrogen receptor (ER)α, underwent a dose-dependent growth arrest (IC(50)=0.65±0.09 and 0.41±0.07 mM, respectively) and accumulation in G(0)–G(1) phase. WEB-2086 also induced morphological and functional changes typical of mature mammary phenotype including (i) cell enlargement and massive neutral lipid deposition (best accomplished in MCF-7 cells); (ii) decrease in motility and active cathepsin D levels (mainly observed in highly invasive MDA-MB-231 cells). The expression of ERα was neither increased nor reactivated in treated MCF-7 or MDA-MB-231 cells, respectively. WEB-2086-induced differentiation in breast cancer cells involved the upregulation of PTEN, a key tumour suppressor protein opposing tumorigenesis, and was apparently independent of p53, PAFR, peripheral benzodiazepine receptor and ERα status. Overall, WEB-2086 can be proposed as an effective antiproliferative and differentiative agent with interesting translational opportunities to treat breast cancers in support to conventional chemotherapy

Application of a filtration- and isolation-by-size technique for the detection of circulating tumor cells in cutaneous melanoma

Analysis of circulating tumor cells (CTC) in the peripheral blood of cutaneous melanoma patients provides information on the metastatic process and potentially improves patient management. The isolation by size of epithelial tumor cells (ISET) is a direct method for CTC identification in which tumor cells are collected by filtration as a result of their large size. So far, ISET has been applied only to CTC detection from epithelial cancer patients, and the technique has never been applied to cutaneous melanoma patients. We herein investigated the presence of CTC by ISET in the peripheral blood of 140 subjects (87 with cutaneous melanomas, 10 subjects undergoing surgery for melanocytic nevi, 5 patients with non-melanoma skin tumors, and 38 healthy volunteers). The identification of the cells trapped in filters as CTC was supported by positivity for immunohistochemical markers and for tyrosinase mRNA by real-time RT-PCR. CTC were neither detected in the controls nor in the in situ melanoma group. In contrast, CTC were shown in 29% of patients with primary invasive melanoma and in 62.5% of metastatic melanoma patients (P<0.01). CTC detection correlated with the presence of mRNA tyrosinase in blood samples, assayed by real-time RT-PCR (P=0.001). CTC detection corroborated by suitable molecular characterization may assist in the identification and monitoring of more appropriate therapies in melanoma patients. © 2010 The Society for Investigative Dermatology

Hardware-accelerated high-resolution video coding in Virtual Network Functions

Network Function Virtualization (NFV) has become a widely acclaimed approach to facilitate the management and orchestration of network services. However, after rapidly achieving a widespread success, NFV is now challenged by the overwhelming demand of computing power originated by the never-ending growth of innovative applications coming from the Internet world. To overcome this problem, the use of h/w acceleration combined with NFV has been proposed. This way, the computing performance of commodity servers can be greatly enhanced, without losing the advantages offered by NFV in service management. In this paper, to demonstrate the potentialities of NFV and h/w acceleration, a Virtual Network Function for video coding (video Transcoding Unit - vTU) is presented. The vTU is accelerated by a General Purpose GPU, and is based on Open Source software packages for media processing. The vTU architecture is firstly described in details. A thorough characterization of its computing performance is then reported, and the obtained results are compared to those achieved with non-accelerated and/or non-virtualized versions of the vTU itself. Also, the performance provided by an original, GPU accelerated version of the VP8 encoder is presented. The activities described in this paper have been carried out within the EU FP7 T-NOVA project