Variability in soil respiration across riparian-hillslope transitions

The spatial and temporal controls on soil CO2 production and surface CO2 efflux have been identified as outstanding gaps in our understanding of carbon cycling. We investigated both across two riparian-hillslope transitions in a subalpine catchment, northern Rocky Mountains, Montana. Riparian-hillslope transitions provide ideal locations for investigating the controls on soil CO2 dynamics due to strong, natural gradients in the factors driving respiration, including soil water content (SWC) and soil temperature. We measured soil air CO2 concentrations (20 and 50 cm), surface CO2 efflux, soil temperature, and SWC at eight locations. We investigated (1) how soil CO2 concentrations differed within and between landscape positions; (2) how the timing of peak soil CO2 concentrations varied across riparian and hillslope zones; and (3) whether higher soil CO2 concentrations necessarily resulted in higher efflux (i.e. did surface CO2 efflux follow patterns of subsurface CO2)? Soil CO2 concentrations were significantly higher in the riparian zones, likely due to higher SWC. The timing of peak soil CO2 concentrations also differed between riparian and hillslope zones, with highest hillslope concentrations near peak snowmelt and highest riparian concentrations during the late summer and early fall. Surface CO2 efflux was relatively homogeneous at monthly timescales as a result of different combinations of soil CO2 production and transport, which led to equifinality in efflux across the transects. However, efflux was 57% higher in the riparian zones when integrated to cumulative growing season efflux, and suggests higher riparian soil CO2 production

Landscape structure, groundwater dynamics, and soil water content influence soil respiration across riparian–hillslope transitions in the Tenderfoot Creek Experimental Forest, Montana

Variability in soil respiration at various spatial and temporal scales has been the focus of much research over the last decade aimed to improve our understanding and parameterization of physical and environmental controls on this flux. However, few studies have assessed the control of landscape position and groundwater table dynamics on the spatiotemporal variability of soil respiration. We investigated growing season soil respiration in a ~393 ha subalpine watershed in Montana across eight riparian–hillslope transitions that differed in slope, upslope accumulated area (UAA), aspect, and groundwater table dynamics. We collected daily-to-weekly measurements of soil water content (SWC), soil temperature, soil CO2 concentrations, surface CO2 efflux, and groundwater table depth, as well as soil C and N concentrations at 32 locations from June to August 2005. Instantaneous soil surface CO2 efflux was not significantly different within or among riparian and hillslope zones at monthly timescales. However, cumulative integration of CO2 efflux during the 83-day growing season showed that efflux in the wetter riparian zones was ~25% greater than in the adjacent drier hillslopes. Furthermore, greater cumulative growing season efflux occurred in areas with high UAA and gentle slopes, where groundwater tables were higher and more persistent. Our findings reveal the influence of landscape position and groundwater table dynamics on riparian versus hillslope soil CO2 efflux and the importance of time integration for assessment of soil CO2 dynamics, which is critical for landscape-scale simulation and modeling of soil CO2 efflux in complex landscapes

Differential soil respiration responses to changing hydrologic regimes

Soil respiration is tightly coupled to the hydrologic cycle (i.e., snowmelt and precipitation timing and magnitude). We examined riparian and hillslope soil respiration across a wet (2005) and a dry (2006) growing season in a subalpine catchment. When comparing the riparian zones, cumulative CO2 efflux was 33% higher, and peak efflux occurred 17 days earlier during the dry growing season. In contrast, cumulative efflux in the hillslopes was 8% lower, and peak efflux occurred 10 days earlier during the drier growing season. Our results demonstrate that soil respiration was more sensitive to drier growing season conditions in wet (riparian) landscape positions

Local Zinc Chloride Release From a Calcium Sulfate Carrier Enhances Fracture Healing.

BACKGROUND: This study examined the efficacy of calcium sulfate (CaSO4) as a carrier for intramedullary delivery of zinc chloride (ZnCl2) to treat fracture healing in a BB Wistar rat model. A non-carrier-mediated injection of 3.0 mg/kg of ZnCl2 has previously been shown to enhance fracture healing. METHODS: A heterogeneous mixture of ZnCl2 and CaSO4 was administered into the intramedullary femoral canal and a mid-diaphyseal femur fracture was created unilaterally. Early and late parameters of fracture healing were assessed using biomechanical testing, radiographic scoring, quantitative histomorphometry (for percentage of new cartilage and bone within the fracture callus), and long-term histologic evaluation. RESULTS: Fractures treated with 1.0 mg/kg of ZnCl2/CaSO4 demonstrated a significantly higher maximum torque to failure compared with both CaSO4 (P = 0.048) and saline (P = 0.005) controls at 4 weeks postfracture (396.4 versus 251.3 versus 178.7 N mm, respectively). Statistically significant increases in torsional rigidity, effective shear modulus, and effective shear stress were also found, as well as a 3.5 times increase in radiographic score (based on bone union). Histologic examination of the fracture callus indicated enhanced chondrogenesis at day 14 postfracture, with increased percent cartilage for the ZnCl2/CaSO4 group compared with saline (P = 0.0004) and CaSO4 (P = 0.0453) controls. Long-term radiographic and histologic evaluation revealed no abnormal bone formation or infection up to 12 weeks postoperatively. CONCLUSIONS: The effective dose of ZnCl2 augmentation for the enhancement of fracture healing in rats was reduced 3-fold in this study compared with previous findings. Furthermore, CaSO4 acted synergistically with ZnCl2 to increase the mechanical strength and stability at the fracture site

The Impact of Investment Arbitration on Investment Treaty Design: Myth Versus Reality

Investor-state arbitration (ISA) has shaped the practice, scholarship and teaching of international investment law, but to what extent has it shaped its substance? According to anecdotal evidence, states change their investment treaties in response to developments in investment arbitration. To separate myth from reality this article empirically investigates the effect of investment arbitration on treaty making through three impact channels: (1) investment clauses, (2) investment claims and (3) investment case law coding close to 1700 international investment agreements (IIA) across 55 clauses. Our analysis sheds new light on several normative debates within the field. First, we find that the omission or inclusion of investment clauses has no material effect on other treaty design elements. This suggests that ISA clauses are procedural add-ons, which bestow investors with enforcement rights, but do not alter the inter-state nature of the treaties’ substantive obligations. Second, contrary to prior anecdotal and empirical evidence, investment claims do not lead to systematic treaty design changes. Most innovation attributed to investment claims actually pre-dates them. Moreover, only in few countries did investment claims trigger treaty design changes. Hence, rather than worrying about overzealous responses by states to “rebalance” IIAs in the face of investment claims, we should be concerned about the field’s path-dependency and its entrenchment in a pre-arbitration architecture. Third, investment case law exerts the strongest impact on treaty making as controversial interpretive outcomes in investment arbitration trigger traceable changes in treaty design. Hence, states are more active in fine-tuning existing commitments than in designing new ones further entrenching IIAs’ path dependency and lack of innovation