Scent Mining: Extracting Olfactory Events, Smell Sources and Qualities

Olfaction is a rather understudied sense compared to the other senses. In NLP, however, there have been recent attempts to develop taxonomies and benchmarks specifically designed to capture smell-related information. In this work, we further extend this research line by presenting a supervised system for olfactory information extraction in English. We cast this problem as a token classification task and build a system that identifies smell words, smell sources and qualities. The classifier is then applied to a set of English historical corpora, covering different domains and written in a time period between the 15th and the 20th Century. A qualitative analysis of the extracted data shows that they can be used to infer interesting information about smelly items such as tea and tobacco from a diachronical perspective, supporting historical investigation with corpus-based evidence

Building a Multilingual Taxonomy of Olfactory Terms with Timestamps

Olfactory references play a crucial role in our memory and, more generally, in our experiences, since researchers have shown that smell is the sense that is most directly connected with emotions. Nevertheless, only few works in NLP have tried to capture this sensory dimension from a computational perspective. One of the main challenges is the lack of a systematic and consistent taxonomy of olfactory information, where concepts are organised also in a multi-lingual perspective. WordNet represents a valuable starting point in this direction, which can be semi-automatically extended taking advantage of Google n-grams and of existing language models. In this work we describe the process that has led to the semi-automatic development of a taxonomy for olfactory information in four languages (English, French, German and Italian), detailing the different steps and the intermediate evaluations. Along with being multi-lingual, the taxonomy also encloses temporal marks for olfactory terms thus making it a valuable resource for historical content analysis. The resource has been released and is freely available

Dexamethasone Induces the Expression and Function of Tryptophan-2-3-dioxygenase in SK-MEL-28 Melanoma Cells

Tryptophan-2,3-dioxygenase (TDO) is one of the key tryptophan-catabolizing enzymes with immunoregulatory properties in cancer. Contrary to expectation, clinical trials showed that inhibitors of the ubiquitously expressed enzyme, indoleamine-2,3-dioxygenase-1 (IDO1), do not provide benefits in melanoma patients. This prompted the hypothesis that TDO may be a more attractive target. Because the promoter of TDO harbors glucocorticoid response elements (GREs), we aimed to assess whether dexamethasone (dex), a commonly used glucocorticoid, modulates TDO expression by means of RT-PCR and immunofluorescence and function by assessing cell proliferation and migration as well as metalloproteinase activity. Our results show that, in SK-Mel-28 melanoma cells, dex up-regulated TDO and its downstream effector aryl hydrocarbon receptor (AHR) but not IDO1. Furthermore, dex stimulated cellular proliferation and migration and poten-tiated MMP2 activity. These effects were inhibited by the selective TDO inhibitor 680C91 and enhanced by IDO1 inhibitors. Taken together, our results demonstrate that the metastatic melanoma cell line SK-Mel-28 possesses a functional TDO which can also modulate cancer cell phenotype di-rectly rather than through immune suppression. Thus, TDO appears to be a promising, tractable target in the management or the treatment of melanoma progression

Unique Associations between Insulin-Like Growth Factor Binding Protein-1, Insulin-Like Growth Factor-1 and T Cell Immunoglobulin Mucin 3 in Successful Twin Pregnancies Conceived with Donor Oocytes

Background and Objectives: To investigate if pregnancies conceived using an oocyte donor necessitate an alteration in immune regulation, we compared concentrations of insulin-like growth factor binding protein (IGFBP)-1, insulin-like growth factor (IGF)-1 and T cell immunoglobulin mucin-3 (Tim-3) in women with ongoing successful twin pregnancies conceived spontaneously, using assisted reproductive technologies that utilized homologous oocytes or with donor oocytes. Differences in levels of these immune modulatory proteins may be magnified and easier to detect in twin as compared to singleton pregnancies. Methods: In this prospective study IGFBP-1 and IGF-1 were measured in sera and Tim-3 in lysates of peripheral blood mononuclear cells (PBMCs) by ELISA. Results: Median IGFBP-1 levels were lower in women with donor oocytes (41.4 ng/ml) as compared to those with a spontaneous conception (51.2 ng/mL) or who conceived with various assisted reproduction protocols using homologous oocytes (52.4 ng/mL) (p < 0.001). IGF-1 and Tim-3 levels were comparable in each group. The IGFBP-1 level was inversely correlated to the IGF-1 concentration only in women with donor oocytes (p = 0.032). IGFBP-1 and Tim-3 levels were similarly negatively correlated in the donor oocyte group (p = 0. 012). Women in the assisted reproduction group who conceived following intracytoplasmic sperm injection were the only other group in which IGFBP-1 and Tim-3 were negatively correlated (p = 0.018). Conclusions: Down-regulation of IGFBP-1 production in pregnancies conceived with donor oocytes may reduce the extent of pro-inflammatory immunity and contribute to successful outcome in totally allogeneic pregnancies

Steps and control mechanisms of human dendritic cell differentiation

Among immune system dendritic cells (DCs), Langerhans cells (LCs) - found in stratified squamous epithelia - express langerin/CD207 and CD1a and contain Birbeck granules, while connective tissue DCs express DC-SIGN intensely. DCs may be generated in vitro from monocytes and from immature haematopoietic precursors; PPAR (peroxisome proliferator activated receptor)-gamma stimulation can direct CD133+ precursors to generate DCs with a LC phenotype [1]. In order to achieve deeper knowledge on human DC subtypes differentiation, circulating CD14+ monocytes have been isolated by immunomagnetic separation and cultured with GM-CSF, IL-4, and TGF-beta for 7 days. Maturation was induced by further culture for 48 h with TNF-alpha, IL-1 beta and IL-6 [2]. Rosiglitazone was used to stimulate PPARgamma. The cells were analysed by phase contrast and electron microscopy and by immunofluorescence. Both with and without rosiglitazone, cells with a dendritic shape were generated together with non-dendritic, elongated cells with the aspect of anti-inflammatory (M2) macrophages. All the generated, mature cells expressed MHC-II intensely. DCs grown with rosiglitazone were CD207-, while those grown without rosiglitazone appeared CD207+; in neither conditions did cells contain Birbeck granules, while in both conditions DCs expressed DC-SIGN, more intensely with than without rosiglitazone. Elongated non-dendritic cells expressed CD14 more intensely than DCs, and CD80, CD83 and CD86 as intensely as DCs. The results indicate that upon addiction of rosiglitazone the differentiation of cultured monocytes to LCs is hampered, at variance with what happens with cultures of CD133+ precursors. They also indicate that the generation of non-dendritic elongated cells, possibly M2 macrophages, can occur in association with that of dendritic cells

Development and characterization of an in vitro model of colorectal adenocarcinoma with MDR phenotype

The major cause of failure in cancer chemotherapy is the development of multidrug resistance (MDR), and the characterization of biological factors involved in this response to therapy is particularly needed. A doxorubicin‐resistant HCT‐8/R clone was selected from sensitive parental cells and characterized analyzing several parameters (cell cycle phase distribution, apoptotic activity, expression, distribution and functionality of the P‐gp efflux pump, the response to other chemotherapy agents, its ultrastructural features, invasiveness, and transcriptomic profile). HCT‐8/R cells showed a peculiar S phase distribution, characterized by a single pulse of proliferation, resistance to drug‐mediated apoptosis, increased expression and functionality of P‐gp and overexpression of stem cell markers (CD44 and aldehyde dehydrogenase 1A2). At the ultrastructural level, HCT‐8/R presented a greater cell volume and several intracytoplasmic vesicles respect to HCT‐8. Moreover, the resistant clone was characterized by cross resistance to other cytotoxic drugs and a greater capacity for migration and invasion, compared to parental cells. Our data reinforce the concept that the MDR phenotype in HCT‐8/R cells is multifactorial and involves multiple mechanisms, representing an interesting tool to understand the biological basis of MDR and to test strategies that overcome resistance to chemotherapy

Inorganic nanoparticles as potential regulators of immune response in dendritic cells

Aim: The spontaneous adsorption of proteins on nanoparticles (NPs) in biological media is exploited to prepare complexes of NPs and proteins from cancer cells’ lysates for application in cancer immunotherapy. Materials &amp; methods: Gold (Au) and silica NPs were synthesized, incubated with cancer cells’ lysates and characterized. Dendritic cells (DCs) were challenged with protein-coated NPs, their maturation, viability and morphology were evaluated and lymphocytes T proliferation was determined. Results: Silica and Au NPs bound different pools of biomolecules from lysates, and are therefore promising selective carriers for antigens. When incubated with immature DCs, NPs were efficiently endocytosed without cytotoxicity. Finally, protein-coated AuNPs promoted DC maturation and DC-mediated lymphocyte proliferation, at variance with lysate alone and protein-coated silica NPs, that did not promote DCs maturation. Conclusion: These results demonstrate that the spontaneous formation of protein coronas on NPs represents a possible approach to fast, easy, cost-effective DCs stimulation. </jats:p