Heavy metal stress tolerance in Enterobacter sp. PR14 is mediated by plasmid

115-121Last two decades have witnessed the significant exploitation of many plant growth-promoting rhizobacteria (PGPR) as bioinoculants and biocontrol agents (BCAs). However, PGPR with potential of producing multiple biocontrol traits along with heavy metal stress tolerance and ACC deaminase activity are expected to control phytopathogens and increase tolerance of crop to heavy metal stress, thus helping in bioremediation of heavy metal ions and reducing ethylene level in the root zone. The present work reports the production of multiple biocontrol traits like ammonia (NH3), hydrogen cyanide (HCN), siderophore (79%), ACC deaminase (0.8 µM/mg/h), chitinase (9.7 U/mL) and tolerance to heavy metal ions (3200 μg/mL) and trace minerals in Enterobacter sp. PR14 isolated from the model organic farm of Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, India. Elimination of plasmid in the organism resulted in the loss of tolerance of heavy metal ions and trace elements, indicating the role of the plasmid in heavy metal tolerance

Heavy metal stress tolerance in Enterobacter sp. PR14 is mediated by plasmid

Last two decades have witnessed the significant exploitation of many plant growth-promoting rhizobacteria (PGPR) as bioinoculants and biocontrol agents (BCAs). However, PGPR with potential of producing multiple biocontrol traits along with heavy metal stress tolerance and ACC deaminase activity are expected to control phytopathogens and increase tolerance of crop to heavy metal stress, thus helping in bioremediation of heavy metal ions and reducing ethylene level in the root zone. The present work reports the production of multiple biocontrol traits like ammonia (NH3), hydrogen cyanide (HCN), siderophore (79%), ACC deaminase (0.8 µM/mg/h), chitinase (9.7 U/mL) and tolerance to heavy metal ions (3200 μg/mL) and trace minerals in Enterobacter sp. PR14 isolated from the model organic farm of Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, India. Elimination of plasmid in the organism resulted in the loss of tolerance of heavy metal ions and trace elements, indicating the role of the plasmid in heavy metal tolerance

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Not AvailableThe abiotic stresses like drought, heavy metal and salts directly or indirectly influence the global environmental pollution and decrease the agricultural productivity. The stress tolerant plant growth promoting rhizobacteria (PGPR) play an important role against the abiotic stresses in terms of enhancing the efficacy of soil, plant growth promotion (PGP). Stress tolerance PGPRs have certain specific PGP properties such as hormones synthesis, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, Indole-3-acetic acid (IAA) production, Abscisic acid (ABA) synthesis, enzymes production, nitrogen fixation, phosphorus (P) and Potassium (K) solubilization, as results which protect various crops during such stress conditions and consequently enhance crop sustainability. Efficient PGPRs isolated from various stress conditions have certainly, more useful against that particular stress. This article highlighted the isolation of various stresses tolerant PGPRs from varieties of crops under different stress conditions and their effect on varieties of crops to enhance their plant growth promotion.Not Availabl

A Quantitative Measure of Conformational Changes in Apo, Holo and Ligand-Bound Forms of Enzymes

Determination of the native geometry of the enzymes and ligand complexes is a key step in the process of structure-based drug designing. Enzymes and ligands show flexibility in structural behavior as they come in contact with each other. When ligand binds with active site of the enzyme, in the presence of cofactor some structural changes are expected to occur in the active site. Motivation behind this study is to determine the nature of conformational changes as well as regions where such changes are more pronounced. To measure the structural changes due to cofactor and ligand complex, enzyme in apo, holo and ligand-bound forms is selected. Enzyme data set was retrieved from protein data bank. Fifteen triplet groups were selected for the analysis of structural changes based on selection criteria. Structural features for selected enzymes were compared at the global as well as local region. Accessible surface area for the enzymes in entire triplet set was calculated, which describes the change in accessible surface area upon binding of cofactor and ligand with the enzyme. It was observed that some structural changes take place during binding of ligand in the presence of cofactor. This study will helps in understanding the level of flexibility in protein-ligand interaction for computer-aided drug designing