Bioinformatics tools for the discovery of new lipopeptides with biocontrol applications

As conventional or chemical pesticides have negative impact on environment and health of both farmer and consumers, it becomes relevant to develop alternative solutions to limit their use. In this context, innovative strategies to accelerate the development of biocontrol agents are welcome. For a decade of years, it has been demonstrated that lipopeptides are very efficient weapons against fungi responsible for crop diseases. Lipopeptides are secondary metabolites, produced by many microorganisms including beneficial rhizobacteria. The lipopeptide biosynthetic pathways include nonribosomal peptide synthetases. These modular enzymatic complexes work as assembly lines to build the peptides step by step, leading to the production of original peptide compounds with specific features as the presence of non proteinogenic monomers and cyclic and branched structures. In this paper, Florine and Norine bioinformatics tools, especially dedicated to non-ribosomal synthetases and their products are presented. Their use is mainly focused on the discovery of lipopeptides produced by Bacillus or Pseudomonas because they seem to represent a versatile reservoir of active secondary metabolites with promising activities for applications in phytosanitary area. © 2018, Koninklijke Nederlandse Planteziektenkundige Vereniging.Peer reviewe

Norine, the knowledgebase dedicated to nonribosomal peptides, is now open to crowdsourcing

International audienceSince its creation in 2006, Norine remains the unique knowledgebase dedicated to non-ribosomal pep-tides (NRPs). These secondary metabolites, produced by bacteria and fungi, harbor diverse interesting biological activities (such as antibiotic, anti-tumor, siderophore or surfactant) directly related to the diversity of their structures. The Norine team goal is to collect the NRPs and provide tools to analyze them efficiently. We have developed a user-friendly interface and dedicated tools to provide a complete bioinformatics platform. The knowledgebase gathers abundant and valuable annotations on more than 1100 NRPs. To increase the quantity of described NRPs and improve the quality of associated annotations , we are now opening Norine to crowdsourc-ing. We believe that contributors from the scientific community are the best experts to annotate the NRPs they work on. We have developed MyNorine to facilitate the submission of new NRPs or modifications of stored ones. This article presents MyNorine and other novelties of Norine interface released since the first publication. Norine is freely accessible from the following URL: http://bioinfo.lifl.fr/NRP

Teor de nitrogênio em sementes de Araucaria angustifolia.

Edição dos resumos do 18º Congresso Brasileiro de Sementes, 2013, Florianópolis

Three-pion exchange: a gap in the nucleon-nucleon potential

The leading contribution to the three-pion exchange nucleon-nucleon potential is calculated in the framework of chiral symmetry. It has pseudoscalar and axial components and is dominated by the former, which has a range of about 1.5 fm and tends to enhance the OPEP. The strength of this force does not depend on the pion mass and hence it survives in the chiral limit.Comment: 20 pages, 5 figures, 1 tabl

Estimativa de parâmetros genéticos em progênies de Cordia thricotoma.

Resum