Integrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp

Two phase olive mill waste (TPOMW) is presently the major waste produced by the olive mill industry. This waste has potential to be used as substrate for solid state fermentation (SSF) despite of its high concentration of phenolic compounds and low nitrogen content. In this work, it is demonstrated that mixtures of TPOMW with winery wastes support the production of lipase by Aspergillus spp. By agar plate screening, Aspergillus niger MUM 03.58, Aspergillus ibericus MUM 03.49 and Aspergillus uvarum MUM 08.01 were chosen for lipase production by SSF. Plackett-Burman experimental design was employed to evaluate the effect of substrate composition and time on lipase production. The highest amounts of lipase were produced by A. ibericus on a mixture of TPOMW, urea and exhausted grape mark (EGM). Urea was found to be the most influent factor for the lipase production. Further optimization of lipase production by A. ibericus using a full factorial design (32) conducted to optimal conditions of substrate composition (0.073 g urea/g and 25% of EGM) achieving 18.67 U/g of lipolytic activity.Jose Manuel Salgado is grateful for Postdoctoral fellowship (EX-2010-0402) of Education Ministry of Spanish Government. Luis Abrunhosa was supported by the grant SFRH/BPD/43922/2008 from Fundacao para a Ciencia e Tecnologia-FCT, Portugal. Authors thank Fundacao para a Ciencia e a Tecnologia (FCT) for financial support through the project FCT Pest-OE/EQB/LA0023/2011

Optimization of Cellulase Production by Trichoderma Strains Using Crude Glycerol as a Primary Carbon Source with a 24 Full Factorial Design

This work focuses on the optimization of cellulase production by two Trichoderma strains. A 24 full factorial design was used to evaluate the effects of four factors in the optimization of cellulase production (filter paper assay—FPA): crude glycerol, microcrystalline cellulose, yeast extract and ammonium sulfate. In fermentation with Trichoderma CMIAT 054 strain the largest FPA (138.48 FPU L−1) occurred with 25.0 g L−1 of cellulose, 10.0 g L−1 of crude glycerol, 1.4 g L− 1 of yeast extract and 3.5 g L− 1 of ammonium sulfate in the culture medium. In tests with Trichoderma CMIAT 041 strain the highest FPA (89.35 FPU L−1) occurred with 25.0 g L−1 of cellulose, 20.0 g L−1 of crude glycerol, 0.6 g L−1 of yeast extract and 1.5 g L−1 of ammonium sulfate in the culture medium. ANOVA showed a correlation coefficient of 93 and 88% for Trichoderma CMIAT 054 and CMIAT 041 strains, respectively. Reduced regression models for the cellulase produced by these strains were obtained. © 2016, Springer Science+Business Media Dordrecht