4 research outputs found

    On-line Measurement And Power Consumption On Gas-liquid Agitated Systems

    No full text
    In some industrial processes there is the necessity of if exhausting a gaseous component in a liquid phase, particularly, in processes that involve aerobic fermentation for production of bioproducts as: agricultural inoculantes, recombinant proteins, antibiotic, vaccines, biopolymmers and others. Through the dispersion of this gaseous component,often the oxygen, one knows that a reduction in the apparent density of the liquid occurs, and it leads to a reduction in the power consumption that is transmitted in comparison to the ungassed liquid. From the on-line measurement of the power effectively transferred to the fluid, made possible for the use of strain-gauges, the present work has as objective to quantify this phenomenon of the power reduction, under different conditions of agitation and aeration. The quantification of the power effectively transferred to the fluid is very important as a parameter for the magnifying of scale of biotechnological processes, that allows transforming an optimized project into laboratory scale, in something with industrial applicability. From the instrumentation of the axis of benches tank of 10L owned by the Institute for Technological Research of State of São Paulo S.A. (IPT), it was built collection of data of power effectively transferred to the fluid. One expects that this study can provide a method of great utility in the forecast of power of agitated tanks and gassed, through the equations that will be offer.GODBOLE, S.P.SHAH, Y.T, Design and Operation of Bubble Column Reactors, In: Cheremisinoff, N.P. Encyclopedia of Fluid Mechanics, 3, Gas-Liquid Flows, Houston, Gulf Publishing Company, 1986, p.1217-1239URENHA, L.C., Agitação e Mistura na Indú stria (2002) Curso de Extensão da Universidade Estadual de Campinas, , UNICAMP, Campinas(1999) 100 anos de Tecnologia, , INSTITUTO DE PESQUISAS TECNOLÓGICAS, IPT, São Paulo. IPT, 191 pINSTITUTO DE PESQUISAS TECNOLÓGICAS - IPT. Utilizaç ão de técnicas modernas de engenharia de processos fermentativos na produção de inoculantes agrícolas para leguminosas. Relatório técnico final 58.255. São Paulo. IPT. 2002. 27

    Microbial Lipid Production: Screening With Yeasts Grown On Brazilian Molasses

    No full text
    Rhodotorula glutinis CCT 2182, Rhodosporidium toruloides CCT 0783, Rhodotorula minuta CCT 1751 and Lipomyces starkeyi DSM 70296 were evaluated for the conversion of sugars from Brazilian molasses into single-cell oil (SCO) feedstock for biodiesel. Pulsed fed-batch fermentations were performed in 1.65 l working volume bioreactors. The maximum specific growth rate (µmax), lipid productivity (Pr) and cellular lipid content were, respectively, 0.23 h−1, 0.41 g l−1 h−1, and 41 % for Rsp. toruloides; 0.20 h−1, 0.27 g l−1 h−1, and 36 % for Rta. glutinis; 0.115 h−1, 0.135 g l−1 h−1, and 27 % for Rta. minuta; and 0.11 h−1, 0.13 g l−1 h−1, and 32 % for L. starkeyi. Based on their microbial lipid productivity, content, and profile, Rsp. toruloides and Rta. glutinis are promising candidates for biodiesel production from Brazilian molasses. All the oils from the yeasts were similar to the composition of plant oils (rapeseed and soybean) and could be used as raw material for biofuels, as well as in food and nutraceutical products.361224332442Almazan, O., Klibansky, M., Otero, M.A., Microbial fat synthesis by Rhodorula Glutiinis from blackstrap molasses in continuous culture (1981) Biotechnol Lett, 3, pp. 663-666. , COI: 1:CAS:528:DyaL38XosVSquw%3D%3DAnschau, A., Xavier, M.C.A., Hernalsteens, S., Franco, T.T., Effect of feeding strategies on lipid production by Lipomyces starkey (2014) Biores Technol, 157, pp. 214-222. , COI: 1:CAS:528:DC%2BC2cXmslaisrc%3DBligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can J Biochem Physiol, 37, pp. 911-917. , PID: 13671378, COI: 1:CAS:528:DyaG1MXhtVSgt70%3DChatzifragkou, A., Fakas, S., Galiotou-Panayotou, M., Komaitis, M., Aggelis, G., Papanikolaou, S., Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi (2010) Eur J Lipid Sci Technol, 112, pp. 1048-1057. , COI: 1:CAS:528:DC%2BC3cXhtFOhsr3ECuellar, M.C., Heijnen, J.J., Wielen, L.A.M.V.D., Large-scale production of diesel-like biofuels – process design as an inherent part of microorganism development (2013) Biotechnol J, 8, pp. 682-689. , PID: 23650260, COI: 1:CAS:528:DC%2BC3sXntVaku74%3DDoran PM (1995) Bioprocess Engineering Principles. In: Fluid Flow and Mixing. Academic Press, pp 129-163Fales, F.W., Evaluation of a spectrophotometric method for determination of total fecal lipid (1971) Clin Chem, 17, pp. 1103-1108. , PID: 5127367, COI: 1:CAS:528:DyaE38Xjt1ShtQ%3D%3DGranger, L.M., Perlot, P., Goma, G., Pareilleux, A., Kinetics of growth and fatty acid production of Rhodotorula glutinis (1992) Appl Biochem Biotechnol, 37, pp. 13-17. , COI: 1:CAS:528:DyaK38XisFCjsr4%3DHaas, M.J., McAloon, A.J., Yee, W.C., Foglia, T.A., A process model to estimate biodiesel production costs (2006) Biores Technol, 97, pp. 667-678Hassan, M., Blanc, P.J., Granger, L.M., Pareilleux, A., Goma, G., Lipid production by an unsaturated fatty acid auxotroph of the oleaginous yeast Apiotrichum Curvatum grown in single stage continuous culture (1993) Appl Microbiol Biotechnol, 40, pp. 483-488. , COI: 1:CAS:528:DyaK2cXktlehsL0%3DHugot, M., Manual da engenharia açucareira (1969) Cozimento, pp. 667-752. , Mestre Jou Publ Ltd, São Paulo:Jacob, Z., Krishnamurthy, M.N., Studies on physicochemical characteristics and fatty acid composition of lipid produced by a strain of Rhodotorulla gracillis CFR-1 (1990) J Am Oil Chem Soc, 67, pp. 642-645. , COI: 1:CAS:528:DyaK3cXmt1Olurk%3DJohnson, V.W., Singh, M., Saini, V.S., Adhikari, D.K., Sista, V., Yadav, N.K., Utilization of molasses for the production of fat by an oleaginous yeast, Rhodotorula glutinis IIP-30 (1995) J Ind Microbiol, 14, pp. 1-4. , COI: 1:CAS:528:DyaK2MXktFWqtbc%3DKoutinas, A.A., Chatzifragkou, A., Kopsahelis, N., Papanikolaou, S., Kookos, I.K., Design and techno-economic evaluation of microbial oil production as a renewable resource for biodiesel and oleochemical production (2014) Fuel, 116, pp. 566-577. , COI: 1:CAS:528:DC%2BC3sXhslKmtrnOLewis, T., Nichols, P.D., McMeekin, T.A., Evaluation of extraction methods for recovery of fatty acids from lipid producing microheterotrophs (2000) J Microbiol Methods, 43, pp. 107-116. , PID: 11121609, COI: 1:CAS:528:DC%2BD3cXot1yju78%3DLi, Y., Zhao, Z., Bai, F., High density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture (2007) Enz Microb Tech, 41, pp. 312-317Lopes, D.C., Neto, A.J.S., Martins, P.A.R., Economic simulation of biodiesel production: SIMB-E tool (2011) Energy Econ, 33, pp. 1138-1145Meade, G.P., Chen, J.C.P., (1977) Cane Sugar Handbook, , Wiley Publ Ltd, New York:Meesters, P., Vanderwal, H., Weusthuis, R., Eggink, G., Cultivation of the oleaginous yeast Cryptococcus curvatus in a new reactor with improved mixing and mass transfer characteristics Surer®) (1996) Biotechnol Tech, 10, pp. 277-282. , COI: 1:CAS:528:DyaK28XislCrsrY%3DMoser, B.R., Vaughn, S.F., Evaluation of alkyl esters from Camelina sativa oil as biodiesel and as blend components in ultra low-sulfur diesel fuel (2010) Biores Technol, 101, pp. 646-653. , COI: 1:CAS:528:DC%2BD1MXht1amu73IPan, J.G., Rhee, J.S., Kinetic and energetic analyses of lipid accumulation in batch culture of Rhodotorula glutinis (1986) J Ferment Technol, 64, pp. 557-560. , COI: 1:CAS:528:DyaL2sXhtFyisr8%3DPradella, J.G.C., Contribuição ao estudo da cinética do crescimento celular e acumulo de lipídios por Rhodotorula gracilis (1980) PhD thesis, , University of Campinas, Campinas:Pradella, J.G.C., Ienczak, J.L., Delgado, C.R., Taciro, M.K., Carbon source pulsed feeding to attain high yield and high produtivity in poly(3-hydroxybutyrate)(PHB) production from soybean oil using Cupriavidus necator (2012) Biotechnol Lett, 34, pp. 1003-1007. , PID: 22315097Ratledge, C., The role of malic enzyme as the provider of NADPH in oleaginous microorganisms: a reappraisal and unsolved problems (2014) Biotechnol Lett, 36, pp. 1557-1568. , PID: 24752812, COI: 1:CAS:528:DC%2BC2cXmsFaktr8%3DRein, P., Cane Sugar Engineering (2013) Molasses Exhaustion, pp. 455-465. , Bartens Publ Ltd, Berlin:Saka, S., Kusdiana, D., Biodiesel fuel from rapeseed oil as prepared in supercritical methanol (2001) Fuel, 80, pp. 225-231. , COI: 1:CAS:528:DC%2BD3cXotVegsL4%3DSaxena, V., Sharma, C.D., Bhagat, S.D., Saini, V.S., Adhikari, D.K., Lipid and fatty acid biosynthesis by Rhodotorula minuta (1998) J Am Oil Chem Soc, 75, pp. 501-505. , COI: 1:CAS:528:DyaK1cXisFyqtbo%3DSuresh, Y., Das, U.N., Long-chain polyunsaturated fatty acids and chemically induced diabetes mellitus: effect of ω-6 fatty acids (2003) Nutrition, 19, pp. 93-114. , PID: 12591540, COI: 1:CAS:528:DC%2BD3sXht1KltLc%3DUnião da Indústria de Cana de Açúcar. Relatório final da safra 2012/2013, Região centro-sul (2014) Acessado em 13 de maio de, p. 2014Vicente, G., Bautista, L.F., Errez, F.J.G., Rodriguez, R., Martinez, V., Rodriguez-Frometa, R.A., Ruiz-Vazquez, R.M., Garre, V., Direct transformation of fungal biomass from submerged cultures into biodiesel (2010) Energy Fuels, 24, pp. 3173-3178. , COI: 1:CAS:528:DC%2BC3cXktFert7w%3DVyas, A.P., Verma, J.L., Subrahmanyam, N., A review on FAME production processes (2010) Fuel, 89, pp. 1-9. , COI: 1:CAS:528:DC%2BD1MXht1WgsL%2FLWiebe, M.G., Koivuranta, K., Penttila, M., Ruohonen, L., Lipid production in batch and fed-batch cultures of Rhodosporidium toruloides from 5 and 6 carbon carbohydrates (2012) BMC Biotechnol, 12 (1), p. 26. , PID: 22646156, COI: 1:CAS:528:DC%2BC3sXht1yrsL4%3DYamauchi, H., Mori, H., Kobayashi, T., Shimizu, S., Mass production of lipids by Lipomyces starkeyi in microcomputer aided fed batch culture (1983) J Ferment Technol, 61, pp. 275-280. , COI: 1:CAS:528:DyaL3sXkvVKmt70%3DYkema, A., Verbree, E.C., Kater, M.M., Smit, H., Optimization of lipid production in the oleaginous yeast Apiotrichum curvatum in whey permeate (1988) Appl Microbiol Biotechnol, 29, pp. 211-218. , COI: 1:CAS:528:DyaL1cXlvVyktr8%3DZabriskie, D.W., Aminger, W.B., Phillips, D.H., (1980) Albano PA, , Traders’ guide to fermentation media formulation Memphis, Tenesse:Zhao, X., Kong, X.L., Hua, Y.Y., Feng, B., Zhao, Z.B., Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi (2008) Eur J Lipid Sci Technol, 110, pp. 405-412. , COI: 1:CAS:528:DC%2BD1cXntFans7k%3DZhao, X., Hu, C., Wu, S., Shen, H., Zhao, Z.K., Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies (2011) J Ind Microbiol Biotechnol, 38, pp. 627-632. , PID: 20711796, COI: 1:CAS:528:DC%2BC3MXkslGqs78%3DZhu, L.Y., Zong, M.H., Wu, H., Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation (2008) Bioresour Technol, 99, pp. 7881-7885. , PID: 18394882, COI: 1:CAS:528:DC%2BD1cXns1arsro%3
    corecore