1,560 research outputs found
Concentration-dependent vascular effects of divalent manganese
Oral Presentation: no. OP9OBJECTIVES: Divalent manganese is a cofactor for soluble guanylyl cyclase (sGC), the enzyme producing cyclic GMP in vascular smooth muscle cells that causes relaxation. Manganese competes with magnesium to activate sGC. These divalent cations can bias the activity of the enzyme to produce cyclic nucleotides other than cyclic GMP, in particular cyclic AMP and cyclic IMP. Cyclic IMP, preferably produced in the presence of magnesium, can cause contraction in ...postprin
Antiretroviral treatment can affect the release of NO and EDCF, but EDH in rat arteries
This journal suppl. entitled: EDHF 2012 - 10th Anniversary MeetingDespite improving clinical outcomes, highly active antiretroviral therapy (HAART) is an
independent potential risk factor for cardiovascular diseases. Currently the recommended HAART
regimen commonly comprises a protease inhibitor (PI) with ritonavir (RTV)-boosting or a nonnucleoside
reverse transcriptase inhibitor (NNRTI), and two nucleoside reverse transcriptase
inhibitors. The present study examined whether or not boosted lopin...postprin
A comparative study of the quality of life of patients with advanced chronic obstructive pulmonary disease and terminal cancer
2005-2006 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe
Endothelium-dependent vasoconstrictor signals requiring activation of soluble guanylyl cyclase in isolated arteries
Conference Theme: Tomorrow's medicines: pharmacology, patients and populationsINTRODUCTION: Thymoquinone causes an endothelium-dependent augmentation of contraction in isolated arteries, similar to that evoked by hypoxia. AIMS: Ex vivo experiments were designed to study the mechanisms underlying this unexpected response. METHODS: Arterial rings with or without endothelium were suspended in organ chambers for isometric tension recording. Certain rings were incubated with inhibitors of nitric oxide …published_or_final_versio
Analysis of the meaning of negligence of care in the context of ethical responsibility and legal liability
2003-2004 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe
Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology
Aims: To assess the potential value of chromosome in situ hybridisation (CISH), Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology to help detect carcinoma cells and precursors. Method: Sixty ThinPrep processed cervical cytology samples were studied: 23 cases within the normal limit, 13 low grade squamous intraepithelial lesions (LSILs), 10 high grade squamous intraepithelial lesions (HSILs), six squamous cell carcinomas, three endocervical adenocarcinomas, two cervical adenosquamous cell carcinomas, and three endometrial adenocarcinomas. CISH was performed with DNA probes specific for the pericentromeric regions of chromosome 11 and 16. Hybridisation signals were visualised with the streptavidin-biotin peroxidase technique. The monoclonal MIB1 and polyclonal TRT-H231 antibodies were used to detect Ki-67 and telomerase immunoreactivity, respectively. Results: Non-specific background staining was almost absent in CISH slides. Normal squamous and glandular cells showed a diploid chromosomal pattern. A relative gain in chromosomes 11 and 16 (aneusomy) was seen in HSIL and the carcinomas (p<0.0001 ). In MIB1 stained smears, normal cells and koilocytes showed inconspicuous immunoreactivity, whereas strongly immunoreactive nuclei were found in cancer cells and HSIL (p<0.0001). Not only carcinoma and HSIL cells, but also some normal cells, showed cytoplasmic staining for telomerase. Conclusions: These preliminary results indicate that ThinPrep processed cervical smears are suitable for CISH and immunocytochemical studies. The neoplastic squamous and glandular cells were easily identified based on nuclear aneusomy and strong Ki-67 immuoreactivity in the context of abnormal nuclear morphology. This is the first study to apply CISH in cervical cytology using an immunoenzymatic approach.published_or_final_versio
Thymoquinone causes endothelium-dependent augmentation of contraction depending on activation of soluble guanylyl cyclase in isolated arteries
Poster - Vascular Responsiveness: no. 4/7INTRODUCTION: Experiments were designed to determine the effects of thymoquinone, an alkaloid with in vivo vasodilator properties, in isolated arteries. METHODS: Rings, with or without endothelium, of rat mesenteric arteries, rat aorta and porcine coronary arteries were suspended in conventional organ chambers for isometric tension recording. Certain rings were incubated with inhibitors of nitric oxide (NO) synthase inhibitor (L-N^G-nitroarginine methyl ester, L-NAME) or soluble guanylyl cyclase (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, ODQ). They were contracted with phenylephrine (rat arteries) or prostaglandin F_2alpha (porcine coronary arteries) and exposed to increasing ...postprin
A pilot controlled trial of a combination of dense cranial electroacupuncture stimulation and body acupuncture for post-stroke depression
Our previous studies have demonstrated the treatment benefits of dense cranial electroacupuncture stimulation (DCEAS), a novel brain stimulation therapy in patients with major depression, postpartum depression and obsessive-compulsive disorder. The purpose of the present study was to further evaluate the effectiveness of DCEAS combined with body acupuncture and selective serotonin reuptake inhibitors (SSRIs) in patients with post-stroke depression (PSD).published_or_final_versio
Initial validation of Chinese Pain Assessment in Advanced Dementia Scale (C-PAINAD)
2007-2008 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe
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