LIPID PROFILE AND LIPID PEROXIDATION IN BRONCHIAL ASTHMA

Background: Bronchial asthma, a chronic inflammatory condition, often triggered by allergy & oxidative stress, initiate lipid peroxidation& enhanced release of arachidonic acid from cell membrane. Regarding lipid profile in bronchial asthma patients many conflicting data were reported. Therefore we have conducted this study. Methodology: 50 clinically diagnosed bronchial asthma cases for lipid profile and lipid peroxidation levels were compared with 50 healthy controls. Results: In our study fasting serum HDL and LDL cholesterol level of cases respectively (52.32mg/dl ±3.58), (99.21mg/dl ±12.49) were found to be significantly higher when compared to controls (44.58mg/dl ±2.58), (103.44mg/dl ±10.55) (p-value <0.05). Mean plasma SOD level of cases (3.42 U/mL±0.74) was significantly lowered than controls (7.16 U/mL±0.52). Serum MDA level of cases (5.91nmol/L±0.88) was higher as compared to controls (3.00nmol/L±0.40). (p-value <0.001) Conclusion: Lipid profile in bronchial asthma patients (raised HDL& decreased LDL) provides some degree of protection to this patients against ischemic heart disease (IHD). But increased in lipid peroxidation as evidenced by decrease plasma SOD & raised serum MDA aggravate airway inflammation, smooth muscle contraction & increased vascular permeability Keywords: Asthma; Oxidative stress; Lipid peroxidation; Lipid profile

LIPID PROFILE AND LIPID PEROXIDATION IN BRONCHIAL ASTHMA

Background: Bronchial asthma, a chronic inflammatory condition, often triggered by allergy & oxidative stress, initiate lipid peroxidation& enhanced release of arachidonic acid from cell membrane. Regarding lipid profile in bronchial asthma patients many conflicting data were reported. Therefore we have conducted this study. Methodology: 50 clinically diagnosed bronchial asthma cases for lipid profile and lipid peroxidation levels were compared with 50 healthy controls. Results: In our study fasting serum HDL and LDL cholesterol level of cases respectively (52.32mg/dl ±3.58), (99.21mg/dl ±12.49) were found to be significantly higher when compared to controls (44.58mg/dl ±2.58), (103.44mg/dl ±10.55) (p-value <0.05). Mean plasma SOD level of cases (3.42 U/mL±0.74) was significantly lowered than controls (7.16 U/mL±0.52). Serum MDA level of cases (5.91nmol/L±0.88) was higher as compared to controls (3.00nmol/L±0.40). (p-value <0.001) Conclusion: Lipid profile in bronchial asthma patients (raised HDL& decreased LDL) provides some degree of protection to this patients against ischemic heart disease (IHD). But increased in lipid peroxidation as evidenced by decrease plasma SOD & raised serum MDA aggravate airway inflammation, smooth muscle contraction & increased vascular permeability Keywords: Asthma; Oxidative stress; Lipid peroxidation; Lipid profile

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Not AvailableAquaporins (AQPs) constitute a super family of major intrinsic proteins (MIPs) and aquaporin 4 (AQP4) is one of the most popular protein, participating in different types of bio-physiological pathways in animal, plant and even in bacteria. Zebrafish, considered as a novel model organism, possess an Aquaporine 4 protein (zAQP4) that shares a high degree of homology with mammalian orthologs which was mostly found to be express in brain, ovary, testis, and low levels in other tissues. To focus more light on AQP4 medical related therapeutic applications, the structure of zAQP4 as a drug target protein is extremely essential and there is no such structural information available in protein data bank (PDB). Herein, for the first time we generate the 3D structure of zAQP4 was modeled through a Homology modeling approach. The root-mean-square deviation (RMSD) of atomic positions between the generated model structure and human AQP4, revealed 69.2% sequence identity over align region with RMSD value 2.723 Å. Structural integrity of the model was assessed using molecular dynamics simulation studies to achieve a better understanding of the progress, structure, and function of AQP4. Finally residual involvements for druggable pocket identification were made and some important residues such as VAL-237, PRO-272, GLN-274, PRO-277, TYR-278, VAL-118, LEU-122 and LEU-126 which shows highest druggability probability (1.0) that can emphasize the importance of aquaporin-4 as a target protein in the field of drug research in near feature.Not Availabl

Not Available

Not AvailableAquaporins (AQPs) constitute a super family of major intrinsic proteins (MIPs) and aquaporin 4 (AQP4) is one of the most popular protein, participating in different types of bio-physiological pathways in animal, plant and even in bacteria. Zebrafish, considered as a novel model organism, possess an Aquaporine 4 protein (zAQP4) that shares a high degree of homology with mammalian orthologs which was mostly found to be express in brain, ovary, testis, and low levels in other tissues. To focus more light on AQP4 medical related therapeutic applications, the structure of zAQP4 as a drug target protein is extremely essential and there is no such structural information available in protein data bank (PDB). Herein, for the first time we generate the 3D structure of zAQP4 was modeled through a Homology modeling approach. The root-mean-square deviation (RMSD) of atomic positions between the generated model structure and human AQP4, revealed 69.2% sequence identity over align region with RMSD value 2.723 Å. Structural integrity of the model was assessed using molecular dynamics simulation studies to achieve a better understanding of the progress, structure, and function of AQP4. Finally residual involvements for druggable pocket identification were made and some important residues such as VAL-237, PRO-272, GLN-274, PRO-277, TYR-278, VAL-118, LEU-122 and LEU-126 which shows highest druggability probability (1.0) that can emphasize the importance of aquaporin-4 as a target protein in the field of drug research in near feature.Not Availabl