Caracterização molecular parcial de um isolado de Prunus necrotic ringspot virus de roseira.

O objetivo deste trabalho foi identificar o agente causal do mosaico da roseira e caracterizar um isolado de PNRSV de roseira

Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.

ABSTRACT There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group. Key words: diagnosis, sequencing, Ilarvirus, PNRSV, ApMV, ArMV RESUMO Inexiste caracterização molecular de isolados brasileiros de Prunus necrotic ringspot virus (PNRSV), exceto aqueles de pessegueiros. Neste trabalho, o agente causal do mosaico da roseira foi determinado e os genes das proteínas de movimento (MP) e capsidial (CP) de um isolado de PNRSV de roseira foram molecularmente caracterizados pela primeira vez no Brasil. As sequências completas de nucleotídeos e de aminoácidos deduzidos da MP e da CP foram alinhadas e comparadas com outros isolados. Análises das sequências de nucleotídeos da MP e da CP do isolado brasileiro de PNRSV de roseira e outros isolados da mesma hospedeira revelaram altas identidades de 96,7% e 98,6%, respectivamente, sendo o isolado Rose-Br classificado no grupo PV32. Palavras-chave: diagnose, sequenciamento, Ilarvirus, PNRSV, ApMV, ArMV

Variáveis agronômicas e variabilidade viral entre videiras sintomáticas e assintomáticas para viroses.

Os objetivos deste trabalho foram avaliar variáveis agronômicas em videiras com e sem sintomas de virose e caracterizar isolados virais destas plantas

Expression of Grapevine leafroll-associated virus 2 coat protein gene in Escherichia coli and polyclonal antibody production.

Grapevine lcafroll-associated vírus 2, GLRaV-2 (Closleroviridae, Closierovirusv causes leafroll symptoms in grapevines and seems also to be involved in the aetiology of a widespread graftincompatibility condition.Suplemento. Resumo

Coat protein gene expression of Grapevine virus B in Escherichia coli and polyclonal antibody production.

Corky bark, a component of the grapevine rugose wood complex, caused by Grapevine virus B, GVB (Flexiviridae, Vitivirus), induces decrease of production, incomplete ripening of grapes and progressive decline.Suplemento. Resumo

Occurrence of cowpea aphid-borne mosaic virus in peanut in Brazil

Surveys of groundnut crops in northeastern Brazil since 1995 showed the occurrence of a hitherto unreported virus disease. Characteristic leaf symptoms were ring spots and blotches. The virus was seed transmitted in groundnut (1/610) and cowpea (47/796). Local and systemic symptoms were observed in cowpea (cv. TVu 3433) known to be susceptible to most cowpea aphid-borne mosaic virus (CABMV) isolates. The virus was transmitted by aphids Toxoptera citricidus and Aphis gossypii. Using degenerate primers, the 3′ terminal region of the viral genome was cloned and sequenced. Sequence analyses of the coat protein and the 3′ untranslated region indicated that the potyvirus was most closely related to CABMV isolates from South Africa, Zimbabwe, and the United States. On the basis of genome analysis, the virus was identified as CABMV. The natural occurrence of CABMV on groundnut has so far not been reported. The significance of this finding especially for germplasm exchange is discusse