EFFECT of FULL AGONISTS FOLLOWING CALCIUM DEPRIVATION in RAT VAS-DEFERENS

ESCOLA PAULISTA MED,DEPT BIOCHEM,CAIXA POSTAL 20372,01000 São Paulo,BRAZILESCOLA PAULISTA MED,DEPT PHARMACOL,CAIXA POSTAL 20372,01000 São Paulo,BRAZILESCOLA PAULISTA MED,DEPT BIOCHEM,CAIXA POSTAL 20372,01000 São Paulo,BRAZILESCOLA PAULISTA MED,DEPT PHARMACOL,CAIXA POSTAL 20372,01000 São Paulo,BRAZILWeb of Scienc

CHANGES in the ADENOHYPOPHYSIS and GENITAL-TRACT of CRYPTORCHID RATS - SOME MORPHOLOGICAL and PHARMACOLOGICAL ASPECTS

ESCOLA PAULISTA MED,DEPT MORPHOL,São Paulo 01000,BRAZILESCOLA PAULISTA MED,DEPT MORPHOL,São Paulo 01000,BRAZILWeb of Scienc

Isolation and identification of angiotensin-like peptides from the plasma of the snake Bothrops jararaca

Two distinct hypertensive peptides were purified and characterized from Bothrops jararaca (Bj) plasma incubated at pH 4, 37 degrees C, 24 hr. These peptides were active on rat and Bj blood pressure, on rat isolated uterus, on guinea-pig isolated ileum and on Bj isolated duodenum. At the releasing conditions no further activities were found for kininases, angiotensinases or angiotensin converting enzymes. the peptides were purified by ethanol/ether extraction, Sephadex G-25 gel filtration, semipreparative reverse-phase (C-18) HPLC and analytical (C-18) HPLC. the amino-acid sequences of the purified peptides corresponded to (Ile(5))AII and (Val(5)-Tyr(9))AI and their molecular masses were confirmed by mass spectrometry as 1046.6 and 1348.0 respectively. the presence oi those two angiotensins on Bj plasma may have some evolutionary significance since (Ile(5))AII is known as a mammalian angiotensin and (Val(5))AII as a non-mammalian one.ESCOLA PAULISTA MED,DEPT BIOFIS,São Paulo,BRAZILESCOLA PAULISTA MED,DEPT BIOFIS,São Paulo,BRAZILWeb of Scienc

BOTHROPAIN - A NOVEL CYSTEINE PEPTIDASE FROM PLASMA of the SNAKE BOTHROPS-JARARACA

ESCOLA PAULISTA MED SCH,DEPT BIOCHEM,CP 20372,BR-04034 São Paulo,BRAZILINST BUTANTAN,SERV FARMACOL,BR-05504 São Paulo,BRAZILESCOLA PAULISTA MED SCH,DEPT BIOCHEM,CP 20372,BR-04034 São Paulo,BRAZILWeb of Scienc