Classification of Acute Myelogenous Leukemia in Blood Microscopic Images Using Supervised Classifier

Abstract: Acute Myelogenous Leukemia (AML) is a subtype of acute leukemia that is prevalent among adults. The age of a person with AML is 64 years. The need for automation of leukemia detection arises since current methods involve manual examination of the blood smear as the first step toward diagnosis. It takes lot of time and its accuracy depends on the operator's ability. in proposed system a simple technique that automatically detects and segments AML in blood smears is presented. The proposed method differs from others in: 1) the simplicity of the developed approach; 2) classification of complete blood smear images as opposed to sub images; and 3) The nucleated cells are segmented and detected by the help of this algorithm. Computer simulation involved the following tests: comparing the impact of Hausdorff dimension on the system before and after the influence of local binary pattern digits and then comparing the performance of the proposed algorithms on sub images and full images, and then with some of the existing systems the result of the proposed system will be compared. Eighty microscopic blood images are detected, The current system will managed to obtain 98% accuracy for the localization of the lymphoblast cells and to separate it from the sub images and complete images

A periodic boundary value problem for nonlinear singular differential systems with ‘maxima’

décembre 20052005/12 (N254)-2005/12.Appartient à l’ensemble documentaire : UnivJeun

Entanglement distillation from Gaussian input states by coherent photon addition

The entanglement between Gaussian entangled states can be increased by non-Gaussian operations. We design a new scheme named coherent photon addition, which can coherently add one photon generated by spontaneous parametric down conversation process to Gaussian quadrature-entangled light pulses created by a non-degenerate optical parametric amplifier. This operation can increase the entanglement of input two-mode Gaussian states as an entanglement distillation, and provides us a new method of non-Gaussian operation. This scheme can also help us to study the decoherence of adding one to two-mode Gaussian states from coherent photon addition to normal photon addition.Comment: 5 pages, 1 figure

High Diversity of the Fungal Community Structure in Naturally-Occurring Ophiocordyceps sinensis

BACKGROUND: Ophiocordyceps sinensis (syn. Cordyceps sinensis), which is a parasite of caterpillars and is endemic to alpine regions on the Tibetan Plateau, is one of the most valuable medicinal fungi in the world. "Natural O. sinensis specimens" harbor various other fungi. Several of these other fungi that have been isolated from natural O. sinensis specimens have similar chemical components and/or pharmaceutical effects as O. sinensis. Nevertheless, the mycobiota of natural O. sinensis specimens has not been investigated in detail. METHODOLOGY/PRINCIPAL FINDINGS: Based on the technique of PCR-single-strand conformation polymorphism (PCR-SSCP), the mycobiota of three different sections (stromata, sclerotia, and mycelial cortices) from natural O. sinensis specimens were investigated using both culture-dependent and -independent methods. For the culture-dependent method, 572 fungal strains were isolated, and 92 putative operational taxonomic units (OTUs) were identified from 226 sequenced strains with the threshold of 97%. For the culture-independent method, 490 fungal clones were identified from about 3000 clones of ITS fragments from the whole-community DNA; based on PCR-SSCP analyses, 266 of these clones were selected to be sequenced, and 118 putative OTUs were detected. The overwhelming majority of isolates/clones and OTUs were detected from mycelial cortices; only a few were detected from stromata and sclerotia. The most common OTUs detected with both methods belonged to Ascomycota; however, only 13 OTUs were detected simultaneously by both methods. Potential novel lineages were detected by each of the two methods. CONCLUSIONS/SIGNIFICANCE: A great number of fungal species present in the mycobiota of naturally-occurring O. sinensis specimens were detected, and many of them may represent undescribed lineages. That only a few of the same OTUs were detected by both methods indicated that different methods should be used. This study increased our understanding about the fungal community structure of this valuable medicinal herb

Uncovering the multifaceted roles played by neutrophils in allogeneic hematopoietic stem cell transplantation

Allogeneic hematopoietic stem cell transplantation (alloHSCT) is a life-saving procedure used for the treatment of selected hematological malignancies, inborn errors of metabolism, and bone marrow failures. The role of neutrophils in alloHSCT has been traditionally evaluated only in the context of their ability to act as a first line of defense against infection. However, recent evidence has highlighted neutrophils as key effectors of innate and adaptive immune responses through a wide array of newly discovered functions. Accordingly, neutrophils are emerging as highly versatile cells that are able to acquire different, often opposite, functional capacities depending on the microenvironment and their differentiation status. Herein, we review the current knowledge on the multiple functions that neutrophils exhibit through the different stages of alloHSCT, from the hematopoietic stem cell (HSC) mobilization in the donor to the immunological reconstitution that occurs in the recipient following HSC infusion. We also discuss the influence exerted on neutrophils by the immunosuppressive drugs delivered in the course of alloHSCT as part of graft-versus-host disease (GVHD) prophylaxis. Finally, the potential involvement of neutrophils in alloHSCT-related complications, such as transplant-associated thrombotic microangiopathy (TA-TMA), acute and chronic GVHD, and cytomegalovirus (CMV) reactivation, is also discussed. Based on the data reviewed herein, the role played by neutrophils in alloHSCT is far greater than a simple antimicrobial role. However, much remains to be investigated in terms of the potential functions that neutrophils might exert during a highly complex procedure such as alloHSCT