Identification of microsatellites markers associated with yield components and quality parameters in sugarcane

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Marker assisted selection depends on the identification of tightly linked association between marker and the trait of interest. In the present work, functional (EST-SSRs) and genomic (gSSRs) microsatellite markers were used to detect putative QTLs for sugarcane yield components (stalk number, diameter and height) and as well as for quality parameters (Brix, Pol and fibre) in plant cane. The mapping population (200 individuals) was derived from a bi-parental cross (IACSP95-3018 x IACSP93-3046) from the IAC Sugarcane Breeding Program. As the map is under construction, single marker trait association analysis based on the likelihood ratio test was undertaken to detect the QTLs. Of the 215 single dose markers evaluated (1:1 and 3:1), 90 (42%) were associated with putative QTLs involving 43 microsatellite primers (18 gSSRs and 25 EST-SSRs). For the yield components, 41 marker/trait associations were found: 20 for height, 6 for diameter and 15 for stalk number. An EST-SSRs marker with homology to non-phototropic hypocotyls 4 (NPH4) protein was associated with a putative QTL with positive effect for diameter as also with a negative effect for stalk number. In relation to the quality parameters, 18 marker trait associations were found for Brix, 19 for Pol, and 12 for fibre. For fibre, 58% of the QTLs detected showed a negative effect on this trait. Some makers associated with QTLs with a negative effect for fibre showed a positive effect for Pol, reflecting the negative correlation generally observed between these traits.1131346140144Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Clusters Of Brazilian Spotted Fever In São Paulo State, Southeastern Brazil. A Review Of Official Reports And The Scientific Literature

[No abstract available]15SUPPL. 2202204Angerami, R.N., Resende, M.R., Feltrin, A.F.C., Brazilian spotted fever: a case series from an endemic area in Southeastern Brazil. Epidemiological aspects (2006) Ann N Y Acad Sci, 1078, pp. 170-172Sexton, D.J., Muniz, M., Corey, G.R., Brazilian spotted fever in Espirito Santo, Brazil: description of a focus of infection in a new endemic region (1993) Am J Trop Med Hyg, 49, pp. 222-226Galvão, M.A.M., Dumler, S., Mafra, C.L., Fatal spotted fever Rickettsiosis, Minas Gerais, Brazil (2003) Emerg Infect Dis, 9, pp. 1402-1405Jones, T.F., Craig, A.S., Paddock, C.D., Family cluster of Rocky Mountain spotted fever (1999) Clin Infect Dis, 28, pp. 853-859Oteo, J.A., Portillo, A., Santibáñez, S., Blanco, J.R., Pérez-Martinez, L., Ibarra, V., Cluster of cases of Rickettsia felis infection from Southern Europe (Spain) diagnosed by PCR (2006) J Clin Microbiol, 44, pp. 2669-267