Recurrent high-biomass blooms of Alexandrium taylorii (Dinophyceae), a HAB species expanding in the Mediterranean.

none9openGIACOBBE M.G., PENNA A., GANGEMI E., MASO' M; GARCES E., FRAGA S., BRAVO I., AZZARO F., PENNA N.Giacobbe, M. G.; Penna, Antonella; Gangemi, E.; Maso', M; Garces, E.; Fraga, S.; Bravo, I.; Azzaro, F.; Penna, N

WATZON: the Italian network of ecohydrology and critical zone observatories

The Italian initiative WATZON (WATer mixing in the critical ZONe) is a network of instrumented sites, bringing together six pre-existing long-term research observatories monitoring different compartments of the Critical Zone - the Earth's permeable near-surface layer from the tops of the trees to the bottom of the groundwater. These observatories cover different climatic and physiographic characteristics over the country, providing information over a climate and eco-hydrologic transect connecting the Mediterranean to the Alps. With specific initial scientific questions, monitoring strategies, databases, and modeling activities, the WATZON observatories and sites is well representative of the heterogeneity of the critical zone and of the scientific communities studying it. Despite this diversity, all WATZON sites share a common eco-hydrologic monitoring and modelling program with three main objectives: 1) assessing the description of water mixing process across the critical zone by using integrated high-resolution isotopic, geophysical and hydrometeorological measurements from point to catchment scale, under different physiographic conditions and climate forcing; 2) testing water exchange mechanisms between subsurface reservoirs and vegetation, and assessing ecohydrological dynamics in different environments by coupling the high-resolution data set from different critical zone study sites of the initiative with advanced ecohydrological models at multiple spatial scales; 3) developing a process-based conceptual framework of ecohydrological processes in the critical zone to translate scientific knowledge into evidence to support policy and management decisions concerning water and land use in forested and agricultural ecosystems. This work provides an overview of the WATZON network, its objectives, scientific questions, and data management, with a specific focus on existing initiatives for linking data and models based on WATZON data

Assessing root water uptake transit time by simulating isotope transport in Hydrus-1D

Stable isotopes (2H and 18O) are common natural tracers for the investigation of water transport in the soil-plant-atmosphere continuum. Isotopic data can be coupled with soil water content data to inversely estimate soil hydraulic and transport parameters. The calibration of a hydrological model by inverse modelling is a prerequisite to determine the temporal origin of xylem water taken by roots. In this study, we used isotopic data to calibrate Hydrus-1D via inverse modelling to simulate one-dimensional water flow and isotope transport in a controlled soil-plant-atmosphere system. We propose the following protocol i) to estimate root water uptake transit time of irrigation water, and ii) to assess the sensitivity of the transit time distribution to the variation in the water available for root uptake. The dataset was obtained from an isotope-tracing experiment carried out between May and July 2018 on an olive tree placed in a pot inside a glasshouse. Meteorological variables and sap flow were monitored at 5-minute intervals, whereas shallow soil moisture (0-6 cm depth) was measured manually with an impedance probe at the daily timescale. The olive tree was irrigated with water of known isotopic composition. The pot surface was covered by a plastic sheet to avoid evaporation. Soil at different depths, twigs, wood cores and root samples were collected weekly for isotopic analyses. Water from soil and the xylem tissues was extracted by cryogenic vacuum distillation. Based on the results of a previous study carried out on the same dataset, we considered that no isotopic fractionation occurred during the water uptake and the transport within the olive tree. We used soil water content and δ18O data at different soil depths to optimize flow (soil hydraulic and root water uptake parameters) and transport (longitudinal dispersivity) parameters. Numerical simulations of isotope transport were validated with sap flow data (compared to actual transpiration) and δ18O in xylem water. Given that the timing of irrigation water for plant transpiration is fundamental for assessing the vulnerability of olive trees to drought, we will be proposing various scenarios based on different irrigation events to mimic drought periods. Based on these scenarios, we will be evaluating the sensitivity of the root water uptake transit time to the different water availability in the soil profile. Afterwards, the same protocol will be exploited to determine the root water uptake transit time for different tree species under various environmental conditions

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Ricerche su un fenomeno eutrofico verificatosi nelle acque della costa marchigiana nel tratto compreso tra Gabicce ed Ancona nel gennaio-febbraio '93.

ACQUA-ARI

The role of dissolved carbohydrates in the northern Adriatic macroaggregate formation

Total dissolved carbohydrates and their monosaccharide and polysaccharide fractions were studied at a fixed sampling point in the northern Adriatic sea off Pesaro, Italy, in 2001 and 2005 when the macroaggregates were absent..

210Po and 210Pb concentration in environmental samples of the Central Adriatic Sea

This study reports the concentrations of important tracers of the marine environment, 210Po and 210Pb, in different matrices from field samples collected at Central Adriatic Sea (Italy), a non-contaminated marine ecosystem. 210Po concentration appears to decrease with increasing distance from the coast and a significant difference in 210Po concentrations in seawater samples at two different depths is not appreciable. 210Po and 210Pb present the same trend in the different periods of the year. 210Po and 210Pb present high concentration factors and high Kd values: in particular 210Po shows values of 2.4 104 in plankton, 3.2 105 in the particulate fraction and 1.1 105 in sediment; 210Pb shows values of 1.3 104 in plankton, 1.8 104 in the particulate fraction and 4.9 104 in sediment. The 210Po/210Pb ratio results 1 in the particulate fraction, sediment and plankton due to a significant fractionation occurring between the two radionuclides during their removal from solution to particle and due to their different biogeochemical cycling pathways in the marine environment. The noticeable accumulation of 210Po in the food chain is not accompanied by an identical accumulation of 210Pb. Due to its biomagnification in the marine food chain, 210Po provides the larges radiation dose to any marine organism under natural conditions. The data reported provide reference values for the Central Adriatic (Mediterranean Sea) coastal environment

Downscaling near-surface soil moisture from field to plot scale: A comparative analysis under different environmental conditions.

Indirect measurements of field-scale (hectometer grid-size) spatial-average near-surface soil moisture are becoming increasingly available by exploiting new-generation ground-based and satellite sensors. Nonetheless, modeling applications for water resources management require knowledge of plot-scale (1–5 m grid-size) soil moisture by using measurements through spatially-distributed sensor network systems. Since efforts to fulfill such requirements are not always possible due to time and budget constraints, alternative approaches are desirable. In this study, we explore the feasibility of determining spatial-average soil moisture and soil moisture patterns given the knowledge of long-term records of climate forcing data and topographic attributes. A downscaling approach is proposed that couples two different models: the Eco-Hydrological Bucket and Equilibrium Moisture from Topography. This approach helps identify the relative importance of two compound topographic indexes in explaining the spatial variation of soil moisture patterns, indicating valley- and hillslope-dependence controlled by lateral flow and radiative processes, respectively. The integrated model also detects temporal instability if the dominant type of topographic dependence changes with spatial-average soil moisture. Model application was carried out at three sites in different parts of Italy, each characterized by different environmental conditions. Prior calibration was performed by using sparse and sporadic soil moisture values measured by portable time domain reflectometry devices. Cross-site comparisons offer different interpretations in the explained spatial variation of soil moisture patterns, with time-invariant valley-dependence (site in northern Italy) and hillslope-dependence (site in southern Italy). The sources of soil moisture spatial variation at the site in central Italy are time-variant within the year and the seasonal change of topographic dependence can be conveniently correlated to a climate indicator such as the aridity index