Characterization and Control of the Microbial Community Affiliated with Copper or Aluminum Heat Exchangers of HVAC Systems

Microbial growth in heating ventilation and air-conditioning (HVAC) systems with the subsequent contamination of indoor air is of increasing concern. Microbes and the subsequent biofilms grow easily within heat exchangers. A comparative study where heat exchangers fabricated from antimicrobial copper were evaluated for their ability to limit microbial growth was conducted using a full-scale HVAC system under conditions of normal flow rates using single-pass outside air. Resident bacterial and fungal populations were quantitatively assessed by removing triplicate sets of coupons from each exchanger commencing the fourth week after their installation for the next 30 weeks. The intrinsic biofilm associated with each coupon was extracted and characterized using selective and differential media. The predominant organisms isolated from aluminum exchangers were species of Methylobacterium of which at least three colony morphologies and 11 distinct PFGE patterns we found; of the few bacteria isolated from the copper exchangers, the majority were species of Bacillus. The concentrations and type of bacteria recovered from the control, aluminum, exchangers were found to be dependent on the type of plating media used and were 11,411–47,257 CFU cm−2 per coupon surface. The concentration of fungi was found to average 378 CFU cm−2. Significantly lower concentrations of bacteria, 3 CFU cm−2, and fungi, 1 CFU cm−2, were recovered from copper exchangers regardless of the plating media used. Commonly used aluminum heat exchangers developed stable, mixed, bacterial/fungal biofilms in excess of 47,000 organisms per cm2 within 4 weeks of operation, whereas the antimicrobial properties of metallic copper were able to limit the microbial load affiliated with the copper heat exchangers to levels 99.97 % lower during the same time period

Use of cultivation-dependent and -independent techniques to assess contamination of central venous catheters: a pilot study

<p>Abstract</p> <p>Background</p> <p>Catheters are the most common cause of nosocomial infections and are associated with increased risk of mortality, length of hospital stay and cost. Prevention of infections and fast and correct diagnosis is highly important.</p> <p>Methods</p> <p>In this study traditional semiquantitative culture-dependent methods for diagnosis of bacteria involved in central venous catheter-related infections as described by Maki were compared with the following culture-independent molecular biological methods: Clone libraries, denaturant gradient gel electrophoresis, phylogeny and fluorescence in situ hybridization.</p> <p>Results</p> <p>In accordance with previous studies, the cultivation of central venous catheters from 18 patients revealed that <it>S. epidermidis </it>and other coagulase-negative staphylococci were most abundant and that a few other microorganisms such as <it>P. aeruginosa </it>and <it>K. pneumoniae </it>occasionally were found on the catheters. The molecular analysis using clone libraries and sequencing, denaturant gradient gel electrophoresis and sequencing provided several important results. The species found by cultivation were confirmed by molecular methods. However, many other bacteria belonging to the phyla <it>Proteobacteria, Firmicutes, Actinobacteria </it>and <it>Bacteroidetes </it>were also found, stressing that only a minor portion of the species present were found by cultivation. Some of these bacteria are known to be pathogens, some have not before been described in relation to human health, and some were not closely related to known pathogens and may represent new pathogenic species. Furthermore, there was a clear difference between the bacterial species found in biofilm on the external (exluminal) and internal (luminal) side of the central venous catheter, which can not be detected by Maki's method. Polymicrobial biofilms were observed on most of the catheters and were much more common than the cultivation-dependent methods indicated.</p> <p>Conclusion</p> <p>The results show that diagnosis based on molecular methods improves the detection of microorganisms involved in central catheter-related infections. The importance of these microorganisms needs to be investigated further, also in relation to contamination risk from improper catheter handling, as only in vivo contaminants are of interest. This information can be used for development of fast and more reliable diagnostic tools, which can be used in combination with traditional methods.</p

Surface Reaction Models of Protocells

Single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we show that compared to cryopreservation, lower quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencin

Renormalization theory of a two dimensional Bose gas: quantum critical point and quasi-condensed state

We present a renormalization group construction of a weakly interacting Bose gas at zero temperature in the two-dimensional continuum, both in the quantum critical regime and in the presence of a condensate fraction. The construction is performed within a rigorous renormalization group scheme, borrowed from the methods of constructive field theory, which allows us to derive explicit bounds on all the orders of renormalized perturbation theory. Our scheme allows us to construct the theory of the quantum critical point completely, both in the ultraviolet and in the infrared regimes, thus extending previous heuristic approaches to this phase. For the condensate phase, we solve completely the ultraviolet problem and we investigate in detail the infrared region, up to length scales of the order (λ3ρ0)-1/2 (here λ is the interaction strength and ρ0 the condensate density), which is the largest length scale at which the problem is perturbative in nature. We exhibit violations to the formal Ward Identities, due to the momentum cutoff used to regularize the theory, which suggest that previous proposals about the existence of a non-perturbative non-trivial fixed point for the infrared flow should be reconsidered

Large-scale moraine deformation at the Athabasca Glacier, Jasper National Park, Alberta, Canada

In this paper the development of a large-scale gravitational deformation involving the eastern lateral moraine of the Athabasca Glacier in Jasper National Park, Alberta, Canada, is described. Interpretation and analysis of sequential aerial photographs indicates that a 540-m-wide segment of the eastern lateral moraine began to deform in the early 1950s; however, significant movement only began in the late 1960s. Since then, the moraine has undergone progressive gravitational deformation leading to a network of fractures, bulging, and the development of a large gap in the moraine crest. Geographic information system analysis of topographic changes between 1967 and 2006 indicates that the displaced volume of the moraine is approximately 9.0×10 5 m 3 . In the last 39 years, the moraine crest has displaced 55 m (1.4 m yr -1 ) down towards the glacier. The development of slope instability is linked to a combination of debuttressing from recent glacier recession, deformation of the moraine, as well as the movement of a large, mobile, debris-mantled slope impinging the upslope margin of the lateral moraine. This case study illustrates the importance of glacial conditioning and local geomorphological factors in creating conditions for large-scale moraine instability in recently deglacierized alpine basins