Dosagem hormonal e avaliação testicular em cachorro-do-mato (Cerdocyun thous) utilizando diferentes protocolos anestésicos

Tree Cerdocyon thous males received different anesthesia protocols: tiletamine-zolazepan (7mg/kg); ketamine-xylazine (12 and 1mg/kg); ketamine-xylazine-atropin (12, 1.0 and 0.04mg/kg), ketamine-midazolam (12 and 0.5mg/kg) and ketamine-acepromazine (12 and 0.1mg/kg) for semen collection by electroejaculation, testosterone hormonal dosages, fine needle aspiration cytology (FNAC), testicular manual evaluation, biometry by caliper and ultrassonography (US). The ejaculates collected by electroejaculation showed urine contamination making impossible the semen evaluation. The M±PD of serum testosterone was 0.74±0.2ng/mL. The cell types found in FNAC were: spermatogonia 13.3±11.5%, primary spermatocytes 5.5±1.1%, secondary spermatocytes 5.5±3.9%, early spermatids 12.8±6.2%, late spermatids 26.2±11.2%, sperm 14.5±4.7% and Sertoli cells 21.8±2.7%. Manual testicular evaluation showed normal consistency of testicles. The M±PD of testicular biometry by caliper was 3.8±1.5cm³ and by US was 1.1±0.3cm³. The animals showed normal spermatogenesis with normal spermatozoa observed in FNAC and normal testicular US

Estradiol and progesterone fecal metabolites analysis in crab-eating-fox (Cerdocyoun thous)

ABSTRACT In this study, four crab-eating fox females (Cerdocyon thous) maintained at the Federal University of Mato Grosso Zoo, Cuiabá, Brazil, were investigated for 12 months, using feces measurement of estradiol and progesterone concentrations. Fecal collections were performed three times a week for hormone extraction. Two methods of analysis, Elisa (EIA) and Radioimmunoassay (RIA), were used in the measurement of progesterone (P4) and estradiol (E2) metabolites. The aim of this study was to compare and validate two different methods of hormone measurement for C. thous. There were no differences regarding the method used. The Radioimmunoassay technique proved to be more sensitive, however, both showed similar results

Semen collection and evaluation of captive coatis (Nasua nasua)

Semen samples (n=105) were collected through eletroejaculation from six adult male coatis (Nasua nasua) between January 2007 and December 2008 at Universidade Federal de Mato Grosso Zoo, Cuiabá, Brazil. Mean values were: volume (mL); concentration (sperm/mL); total motility (%); progressive sperm motility (scale, 0-5); live spermatozoa (%); acrossome integrity (%); primary defects (%); and secondary defects (%). There was high correlation between total motility and live sperm; total motility and progressive sperm motility; total motility and acrossome integrity; live sperm and progressive motility; live sperm and acrossome integrity and volume and concentration. The method for semen collection was considered safe and efficient. It can be used for the evaluation of breeding potential of coati in captivity and for the establishment of new assisted reproductive technology (ART) for threatened neotropical carnivores species