Infecção experimental de cães com amostras de Babesia canis isoladas em Minas Gerais

The objective of the present study was to evaluate parasitemia and packed cell volume patterns of dogs experimentally inoculated with two isolates of Babesia canis: one from Belo Horizonte (BH) and the other from Lavras (Lv), Minas Gerais State, Brazil. Both isolates showed similar patterns, with the peak of parasitemia occurring three days post-infection. From the fourth day, parasitemia was detected in low levels (0.01%) with small periodical increases. The packed cell volume decreased after the parasitemia beginning, with oscillations during the experimental period. All dogs remained apparently normal, except one, which had been inoculated with the BH isolate and presented classical clinical signs of babesiosis (weakness, anemia, hemoglobinuria and depression). The results suggest that the studied isolates have low pathogenicity, and point to the need for further studies aiming to characterize the subspecies of Brazilian isolates

Dopplerfluxometria das artérias renais: valores normais das velocidades sistólica e diastólica e do índice resistivo nas artérias renais principais Renal arteries Dopplerfluxometry: normal systolic and diastolic flow velocities and resistive index values in the main renal arteries

Dopplerfluxometry of renal arteries has been used to estimate renal perfusion in humans. The aim of this study was to use Dopplerfluxometry technique to calculate the resistive index of main renal arteries in dogs, measuring their systolic and diastolic blood flow velocities. Twenty (10 males, 10 females), adult mongrel dogs, were used in this study. The dogs were submitted to Doppler sonographic evaluation of left and right main renal arteries. The systolic and diastolic blood flow velocities, expressed (in centimeters per second) as mean and standard deviation were 79.96± 8.82 and 28.86± 5.11 in the right main renal artery and 80.22± 6.99 and 29.62± 4.14 in the left main renal artery. The value of resistive index expressed as mean ± standard deviation was 0.64± 0.04 for the right main renal artery and 0.63± 0.028 in the left main renal artery

Manutenção in vitro de células IDE8 em dois tipos de soro bovino In vitro maintenance of IDE8 cells using two types of bovine serum

<abstract language="eng">The present study had the objective of defining the culture conditions, optimizing the maintenance and expansion of an IDE-8 cell line in Brazil, with the aim to propose its use as a model for in vitro infection and multiplication of Brazilian strains of rickettsia and other hemoparasites. The supplementation of IDE-8 cells with two distinct fetal bovine sera (a Brazilian and an imported) was evaluated. Culture media were changed weekly and subcultures were carried out every 15 days. The development of cultures and subcultures was evaluated by the percentage of viability and cellular morphology. The results indicate that the imported SFB can be replaced by the Brazilian SFB one, as no significant differences (P<0.05) were seen among culture viabilities