Osmophore Structure And Phylogeny Of Cirrhaea (orchidaceae, Stanhopeinae)

Phylogenetic relationships and osmophore evolution of six Cirrhaea spp. were studied. Floral morphology was analysed using fresh flowers, and osmophore anatomy was determined on the basis of fixed flowers. Phylogenetic relationships of Cirrhaea were inferred on the basis of internal transcribed spacer (ITS), matK and trnL-F regions using maximum parsimony and Bayesian analyses. Floral morphology and osmophore structure vary among species. All Cirrhaea osmophores have a secretory epidermis without papillae. Cirrhaea is monophyletic and includes three subclades: (1) C.dependens/C.nasuta, with a secretory cylindrical protuberance at the base of the labellar midlobe; (2) C.fuscolutea/C.longiracemosa, with a secretory tissue at the base of the shell-shaped midlobe; and (3) C.loddigesii/C.seidelii with secretory tissue on the inner surface of the lateral lobes. The features of the flowers and osmophores in Cirrhaea spp. extend our knowledge of the diversity of secretory structures in Stanhopeinae, and demonstrate that floral morphology reflects phylogenetic relationships in Cirrhaea.1763369383Antón, S., Kamińska, M., Stpiczyńska, M., Comparative osmophore structure in the flower of Stanhopea graveolens Lindley and Cycnoches chlorochilon Klotzsch (Orchidaceae) (2012) Acta Agrobotanica, 65, pp. 11-22Ascensão, L., Francisco, A., Cotrim, H., Pais, M.S., Comparative structure of the labellum in Ophrys fusca and O.lutea (Orchidaceae) (2005) American Journal of Botany, 92, pp. 1059-1067Barros, F., Vinhos, F., Rodrigues, V.T., Barberena, F.F.V.A., Fraga, C.N., Pessoa, E.M., Forster, W., Menini Neto, L., Orchidaceae (2014) Lista de Espécies da Flora do Brasil, , http://floradobrasil.jbrj.gov.br/jabot/floradobrasil, Jardim Botânico do Rio de Janeiro. Available at:Cseke, L.J., Kaufman, P.B., Kirakosyan, A., The biology of essential oils in the pollination of flowers (2007) Natural Product Communications, 2, pp. 1317-1336Curry, K.J., McDowell, L.M., Judd, W.S., Stern, W.L., Osmophores, floral features, and systematics of Stanhopea (Orchidaceae) (1991) American Journal of Botany, 78, pp. 610-623Curry, K.J., Stern, W.L., McDowell, L.M., Osmophore development in Stanhopea anfracta and S.pulla (Orchidaceae) (1988) Lindleyana, 3, pp. 212-220Davies, K.L., Stpiczyńska, M., Labellar micromorphology of Bifrenariinae Dressler (Orchidaceae) (2006) Annals of Botany, 98, pp. 1215-1231Davies, K.L., Stpiczyńska, M., Micromorphology of the labellum and floral spur of Cryptocentrum Benth. and Sepalosaccus Schltr. (Maxillariinae: Orchidaceae) (2007) Annals of Botany, 100, pp. 797-805Davies, K.L., Stpiczyńska, M., Labellar micromorphology of two euglossine-pollinated orchid generaScuticaria Lindl. and Dichaea Lindl (2008) Annals of Botany, 102, pp. 805-824Davies, K.L., Stpiczyńska, M., Comparative histology of floral elaiophores in the orchids Rudolfiella picta (Schltr.) Hoehne (Maxillariinae sensu lato) and Oncidium ornithorhynchum H.B.K. (Oncidiinae sensu lato) (2009) Annals of Botany, 104, pp. 221-234Davies, K.L., Stpiczyńska, M., Comparative labellar anatomy of resin-secreting and putative resin-mimic species of Maxillaria s.l. (Orchidaceae: Maxillariinae) (2012) Botanical Journal of the Linnean Society, 170, pp. 405-435Davies, K.L., Turner, M.P., Gregg, A., Lipoidal labellar secretions in Maxillaria Ruiz & Pav. (Orchidaceae) (2003) Annals of Botany, 91, pp. 439-446Doyle, J.J., Doyle, J.S., A rapid DNA isolation procedure for small quantities of fresh leaf tissue (1987) Phytochemistry Bulletin, Botanical Society of America, 19, pp. 11-15Dressler, R.L., Pollination by euglossine bees (1968) Evolution, 22, pp. 202-212Dressler, R.L., (1981) The orchids - natural history and classification, , Cambridge: Harvard University PressEndress, P.K., (1994) Diversity and evolutionary biology of tropical flowers, , Cambridge: Cambridge University PressFaegri, K., van der Pijl, L., (1979) The principles of pollination ecology, , Oxford: Pergamon PressFahn, A., (1979) Secretory tissues in plants, , London: Academic PressFelsenstein, J., Confidence limits on phylogenies: an approach using the bootstrap (1985) Evolution, 39, pp. 783-791Fitch, W.M., Toward defining the course of evolution: minimum change for a specific tree topology (1971) Systematic Zoology, 20, pp. 406-416Francisco, A., Ascensão, L., Structure of the osmophore and labellum micromorphology in the sexually deceptive orchids Ophrys bombyliflora and Ophrys tenthredinifera (Orchidaceae) (2013) International Journal of Plant Sciences, 174, pp. 619-636Gerlach, D., (1969) Botanische Mikrotechnik, , Stuttgart: Georg Thieme VerlagGerlach, G., Subtribus: Stanhopeinae (1999) Die Orchideen III/A, pp. 2315-2435. , Schlechter R, ed. Berlin: Paul PareyGovaerts, R., (1999) World checklist of seed plants 3 (1, 2a & 2b): 1-1532, , Deurne: Continental PublishingGovaerts, R., World checklist of monocotyledons database (2003), ACCESS: 1-71827. Kew: The Board of Trustees of the Royal Botanic GardensJohansen, D.A., (1940) Plant microtechnique, , New York: McGraw HillJohnson, L.A., Soltis, D.E., Phylogenetic inference in Saxifragaceae sensu stricto and Gilia (Polemoniaceae) using matK sequences (1995) Annals of the Missouri Botanical Garden, 82, pp. 149-175Lillie, R.D., (1965) Histopathologic technique and practical histochemistry, , 3rd edn. New York: McGraw-HillMartini, P., Schlindwein, C., Montenegro, A., Pollination, flower longevity, and reproductive biology of Gongogra quinquenervis Ruíz and Pavón (Orchidaceae) in an Atlantic forest fragment of Pernambuco, Brazil (2003) Plant Biology, 5, pp. 495-503Mickeliunas, L., Pansarin, E.R., Sazima, M., Biologia floral, melitofilia e influência de besouros Curculionidae no sucesso reprodutivo de Grobya amherstiae Lindl. (Orchidaceae: Cyrtopodiinae) (2006) Revista Brasileira de Botânica, 29, pp. 251-258Neubig, K.M., Whitten, W.M., Williams, N.H., Blanco, M.A., Endara, L., Burleigh, J.G., Silveira, K., Chase, M.W., Generic recircumscriptions of Oncidiinae (Orchidaceae: Cymbidieae) based on maximum likelihood analysis of combined DNA datasets (2012) Botanical Journal of the Linnean Society, 168, pp. 117-146Nunes, C.E.P., Castro, M.M., Galetto, L., Sazima, M., Anatomy of the floral nectary of ornithophilous Elleanthus brasiliensis (Lindl.) Rchb.f. (Orchidaceae: Sobralieae) (2013) Botanical Journal of the Linnean Society, 171, pp. 764-772Pansarin, E.R., (2000) Biologia reprodutiva e morfologia floral de espécies de Orchidaceae em diferentes ambientes no Estado de São Paulo, , Dissertação de mestrado, Universidade Estadual de Campinas, CampinasPansarin, E.R., Aguiar, J.M.R.B.V., Pansarin, L.M., Floral biology and histochemical analysis of Vanilla edwallii Hoehne (Orchidaceae: Vanilloideae): an orchid pollinated by Epicharis (Apidae: Centridini) (2014) Plant Species Biology, 29, pp. 242-252Pansarin, E.R., Amaral, M.C.E., Biologia reprodutiva e polinização de duas espécies de Polystachya no sudeste do Brasil: evidência de pseudocleistogamia em Polystachyeae (Orchidaceae) (2006) Revista Brasileira de Botânica, 29, pp. 423-432Pansarin, E.R., Amaral, M.C.E., Reproductive biology and pollination mechanisms of Epidendrum secundum (Orchidaceae). Floral variation: a consequence of natural hybridization? (2008) Plant Biology, 10, pp. 211-219Pansarin, E.R., Amaral, M.C.E., Reproductive biology and pollination of southeastern Brazilian Stanhopea Frost ex Hook. (Orchidaceae) (2009) Flora, 204, pp. 238-249Pansarin, E.R., Bittrich, V., Amaral, M.C.E., At daybreak - reproductive biology and isolating mechanisms of Cirrhaea dependens (Orchidaceae) (2006) Plant Biology, 8, pp. 494-502Pansarin, L.M., Castro, M., Sazima, M., Osmophore and elaiophores of Grobya amherstiae (Catasetinae, Orchidaceae) and their relation to pollination (2009) Botanical Journal of the Linnean Society, 159, pp. 408-415Pansarin, L.M., Pansarin, E.R., Sazima, M., Reproductive biology of Cyrtopodium polyphyllum (Orchidaceae): a Cyrtopodiinae pollinated by deceit (2008) Plant Biology, 10, pp. 650-659Posada, D., jModelTest: phylogenetic model averaging (2008) Molecular Biology and Evolution, 25, pp. 1253-1256Pridgeon, A.M., Cribb, P., Chase, M.W., Rasmussen, F.N., (2009) Genera Orchidacearum, vol. 5, , New York: Oxford University PressPridgeon, A.M., Stern, W.L., Osmophores of Scaphosepalum (Orchidaceae) (1985) Botanical Gazette, 146, pp. 115-123Ronquist, F., Huelsenbeck, J.P., MrBayes 3: Bayesian phylogenetic inference under mixed models (2003) Bioinformatics, 19, pp. 1572-1574Singer, R.B., Sazima, M., Abelhas Euglossini como polinizadoras de orquídeas na região de Picinguaba, São Paulo, Brasil (2004) Orquidologia sul-americana: uma compilação científica, pp. 175-187. , Barros F, Kerbauy GB, eds. São Paulo: Secretaria do Meio AmbienteStern, W.L., Curry, K.J., Pridgeon, A.M., Osmophores of Stanhopea (Orchidaceae) (1987) American Journal of Botany, 74, pp. 1323-1331Stpiczyńska, M., Anatomy and ultrastructure of osmphores of Cymbidium tracyanum Rolfe (Orchidaceae) (1993) Acta Societatis Botanicorum Poloniae, 62, pp. 5-9Stpiczyńska, M., Osmophores of the fragrant orchid Gymnadenia conopsea L. (Orchidaceae) (2001) Acta Societatis Botanicorum Poloniae, 70, pp. 91-96Sun, Y., Skinner, D.Z., Liang, G.H., Hulbert, S.H., Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA (1994) Theoretical and Applied Genetics, 89, pp. 26-32Swofford, D.L., (2001) PAUP: phylogenetic analysis using parsimony version 4.b.8, , Sunderland, MA: Sinauer AssociatesTaberlet, P., Gielly, L., Pautou, G., Bouvet, J., Universal primers for amplification of three non-coding regions of chloroplast DNA (1991) Plant Molecular Biology, 17, pp. 1105-1109Teixeira, S.P., Borba, E.L., Semir, J., Lip anatomy and its implications for the pollination mechanisms of Bulbophyllum species (Orchidaceae) (2004) Annals of Botany, 93, pp. 499-505Thiele, K., The holy grail of the perfect character: the cladistics treatment of morphometric data (1991) Cladistics, 9, pp. 275-304Vogel, S., Die Duftdrüsen der Orchideen (1962) Akademie der Wissenschaften und der Literatur: Abhandlungen der mathematisch-naturwissenschaftlichen Klasse, 10, pp. 603-763Vogel, S., Duftdrüsen im Dienste der Bestäubung: Über Bau und Funktion der Osmophoren (1963) Abhandlungen der Mathematisch-Naturwissenschaftlichen Klasse: Akademie der Wissenschaften und der Literatur Mainz, Jahrgang, 1962, pp. 599-763Vogel, S., Das sexuelle Anlockungsprinzip der Catasetinen- und Stanhopeen-Blüten und die wahre Funktion ihres sogenannten Futtergewebes (1963) Österreichische Botanische Zeitschrift, 100, pp. 308-337Wiens, J.J., Character analysis in morphological phylogenetics: problems and solutions (2001) Systematic Biology, 50, pp. 689-699Whitten, W.M., Williams, N.H., Chase, M.W., Subtribal and generic relationship of Maxillarieae (Orchidaceae) with emphasis on Stanhopeinae: combined molecular evidence (2000) American Journal of Botany, 87, pp. 1842-1856Wiemer, A.P., Benitez-Vieyra, S., Cocucci, A.A., Raguso, R.A., Sérsic, A.N., A simple floral fragrance and unusual osmophore structure in Cyclopogon elatus (Orchidaceae) (2009) Plant Biology, 11, pp. 506-514Williams, N.H., The biology of orchids and euglossine bees (1982) Orchid biology and perspectives, pp. 119-171. , Arditti J, ed. Ithaca, NY: Cornell University PressWilliams, N.H., Dodson, C.H., Selective attraction of male euglossine bees to orchid floral fragrances and its importance in long distance pollen flow (1972) Evolution, 26, pp. 84-95Wortley, A.H., Scotland, R.W., The effect of combining molecular and morphological data in published phylogenetic analyses (2006) Systematic Biology, 55, pp. 677-68