Characterization Of Storage Cell Wall Polysaccharides From Brazilian Legume Seeds And The Formation Of Aqueous Two-phase Systems

Cell wall storage polysaccharides from Brazilian legume seeds of Dimorphandra mollis, Schizolobium parahybum (galactomannans), Copaifera langsdorffii, Hymenaea courbaril (xyloglucans) and the galactan from cotyledons of the Mediterranean species Lupinus angustifolius were extracted and their apparent molecular masses were determined by high-performance size exclusion chromatography analysis. They were, to a large degree, polydisperse, showing molecular masses that varied from 100 000 to 2 000 000. Polyethylene glycol (PEG, 1500, 4000, 6000 and 8000), sodium citrate and dextran (73 000, 60 000-90 000, 505 000 and 2 000 000) were used for investigating phase formation with the seed polysaccharides. Galactomannans and xyloglucans demonstrated phase formation with sodium citrate concentrations lower than 30%, as well as dextrans and polyethylene glycol, and formed gels in the presence of high concentrations of sodium citrate (above 30%). Galactan did not promote phase formation with any of the reagents used. On the basis of the results obtained, the possibility of using legume seed polysaccharides for the partitioning and purification of polysaccharide enzymes in aqueous two-phase systems is suggested.6801-2255261Reid, J.S.G., (1985) Biochemistry of Storage Carbohydrate in Green Plants, pp. 265-288. , Academic Press, LondonBuckeridge, M.S., Dietrich, S.M.D., (1990) Revta. Brasil. Bot., 13, p. 109Reid, J.S.G., Meier, H.A., (1970) Z. Pflanzenphysiol, 62, p. 89Bailey, R.W., (1971) Chemotaxonomy of the Leguminosae, , J.B. Harborne (Editor), DB & BLT, Academic Press, LondonDea, I.C.M., Clark, A.H., McCleary, B.V., (1986) Carbohydr. Res., 147, p. 275Buckeridge, M.S., Reid, J.S.G., (1994) Planta, 192, p. 192Edwards, M., Dea, I.C.M., Bulpin, P.V., Reid, J.S.G., (1985) Planta, 163, p. 133York, W.S., Von Halbeek, H., Darvill, A.G., Albershein, P., (1990) Carbohydr. Res., 200, p. 9Hirst, E.L., Jones, J.K.N., Walder, W., (1947) J. Chem. Soc., p. 1225Kula, M.-R., (1979) Applied Biochemistry and Bioengineering, 2, pp. 71-95. , J. Wingard Jr., L.B.E. Katchalski-Kotzin and L. Goldstein (Editors), Academic Press, New YorkKula, M.-R., (1985) Comprehensive Biotechnology, 2. , C.L. Cooney and A.E. Humphrey (Editors), Pergamon Press, OxfordKula, M.-R., Kroner, K.H., Hustedt, H., Schutte, H., (1981) Ann. NY Acad. Sci., 369, p. 341Kula, M.-R., (1990) Bioseparation, 1, p. 181Aurol, D., (1989), Ph.D. Thesis presented at the Biochemical Engineering Department of the Institut National des Sciences AppliquésAlbertsson, P.A., Tjerneld, F., (1994) Methods Enzymol., 228, p. 3Albertsson, P.-A., Partition of Cell Particles and Macromolecules, p. 1971. , Wiley Interscience, New York, 3rd edZaslavsky, B.Y., Miheeva, L.M., Mesteckina, N.M., Rogozhin, S.V., (1982) J. Chromatogr., 253, p. 149Franco, T.T., Andrews, B.A., Cascone, O., Hodgson, C., Andrews, A.T., Asenjo, J.A., (1990) Separations for Biotechnology, 2. , D.L. Pyle (Editor), SCI, Elsevier, LondonFranco, T.T., Andrews, A.T., Asenjo, J.A., (1995) Biotech. Bioeng., 49. , in pressFranco, T.T., Andrews, A.T., Asenjo, J.A., (1995) Biotech. Bioeng., 49. , in pressKroner, K.H., Hustedt, H., Kula, M.-R., (1982) Biotech. Bioeng., 24, p. 1015Tjerneld, F., Berner, S., Cajarville, A., Johansson, G., (1986) Enzyme Microb. Technol., 8, p. 417Venancio, A., Teixeira, J.A., (1993) Biotech. Prog., 9, p. 635Garnier, L., Schorsh, C., Doublier, J.-L., (1995) Carbohydr. Polymers,, , in pressDea, I.C.M., Morrison, A., (1975) Advan. Carbohydr. Chem. Biochem., 31, p. 24