In vitro testing of estragole in HepG2 cells: Cytokinesis-block micronucleus assay and cell-cycle analysis

The alkenylbenzene estragole (systematic name, 1-allyl-4-methoxybenzene) is a natural component of essential oils from various spices and herbs, including fennel, and it is used as a food and beverage flavouring agent. Estragole has been reported to be hepatocarcinogenic at high doses in rodents. However, in a previous in vitro study, we found that estragole did not exhibit cytotoxic effects after 4 hours of exposure, nor did it induce DNA damage or apoptosis in human HepG2 hepatoblastoma cells. As fennel tea is widely used for symptomatic treatment of spasmodic gastrointestinal conditions in infants, we aimed at further assessing its safety in a different experimental setting. We thus searched for possible cytogenetic effects and interference with cell-cycle progression in the same human hepatoblastoma cell line. Estragole did not show any clastogenic/aneugenic activities in the cytokinesis-block micronucleus assay, and no effects on cell-cycle checkpoints were observed

Anti-inflammatory and analgesic effects of Spartium junceum L. flower extracts: a preliminary study

Our objective was to study the anti-inflammatory and analgesic activity of extract of Spartium junceum L. flowers. Samples of flowers were collected from wild plants, dried, powdered, and extracted with hexane and methanol. The extracts were evaporated to dryness and then suspended in suitable solvent. They were then tested for anti-inflammatory activity in the carrageenin rat paw edema test and for analgesic activity in the Randall and Selitto mechanical pressure test and in the tail-flick test. Twenty-four hours after treatment, the gastric mucosa of each rat was observed macroscopically. Based on these results the hexane extract was fractioned by column chromatography, and the fractions obtained were tested in the same way. The results showed good anti-inflammatory activity only for a single fraction of the hexane extract, while all the extracts and all the other hexane fractions showed both peripheral and central analgesic activity. In rats treated with the tested compounds hyperemia and ulcers were absent. The data from this preliminary study reveal interesting pharmacological properties of S. junceum L. flowers extract related to the marked analgesic activity and the absence of gastric ulcerogenic activity

Analysis of Deterioration in the Crypt of the Abbey of Montecorona with Integrated Methods

A study of the problem of deterioration in the crypt of the Abbey of Montecorona (Perugia, Northen Umbria-Italy) is proposed, with the aim to achieve a knowledge of the indoor and subsoil conditions. First of all we studied the microbial biodiversity of the crypt, analyzing the presence of microorganisms by microscopic and cultivation methods. Then we investigated the influence of the environment upon colonisation and growth of those micro-organisms, monitoring the microclimate, especially the thermo-hygrometric conditions. Various Ground Penetrating Radar (GPR) System surveys were carried out in order to localise archaeological remains and wet buried structures involved in the deterioration process and, by means a velocity analysis, we evaluated the underground water content. Microclimatic, biological and geophysical results were compared, in order to eliminate the ambiguity inherent each method. The results permits us to identify properly the causes of the deterioration in the crypt