104 research outputs found
Novel AroA from Pseudomonas putida Confers Tobacco Plant with High Tolerance to Glyphosate
Glyphosate is a non-selective broad-spectrum herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, also designated as AroA), a key enzyme in the aromatic amino acid biosynthesis pathway in microorganisms and plants. Previously, we reported that a novel AroA (PpAroA1) from Pseudomonas putida had high tolerance to glyphosate, with little homology to class I or class II glyphosate-tolerant AroA. In this study, the coding sequence of PpAroA1 was optimized for tobacco. For maturation of the enzyme in chloroplast, a chloroplast transit peptide coding sequence was fused in frame with the optimized aroA gene (PparoA1optimized) at the 5âČ end. The PparoA1optimized gene was introduced into the tobacco (Nicotiana tabacum L. cv. W38) genome via Agrobacterium-mediated transformation. The transformed explants were first screened in shoot induction medium containing kanamycin. Then glyphosate tolerance was assayed in putative transgenic plants and its T1 progeny. Our results show that the PpAroA1 from Pseudomonas putida can efficiently confer tobacco plants with high glyphosate tolerance. Transgenic tobacco overexpressing the PparoA1optimized gene exhibit high tolerance to glyphosate, which suggest that the novel PpAroA1 is a new and good candidate applied in transgenic crops with glyphosate tolerance in future
Herbicide-resistant cotton (Gossypium hirsutum) plants: an alternative way of manual weed removal
Prevenção de injĂșrias causadas por glyphosate em soja RR por meio do uso de aminoĂĄcido
Characterising microbial protein test substances and establishing their equivalence with plant-produced proteins for use in risk assessments of transgenic crops
Synthesis, enzymology, and pharmacology of novel oxygenase substrates and inhibitors: dopamine [beta]-monooxygenase and protocatechuate 3,4-dioxygenase
Ph.D.Sheldon W. Ma
Patent Protection v. Tortious Liability - Confronting the Legal Liability for Tortious Behavior with Intellectual Property Protection Under Patent Laws: Society's Legal Tradeoff with GMO-Based Patent Rights and the Damage the Underlying Technology and Commercial Behavior Creates
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