21 research outputs found
Gaze shift duration, independent of amplitude, influences the number of spikes in the burst for medium-lead burst neurons in pontine reticular formation
Evolution, Not Revolution: Measurement and Management of Health Outcomes in New Zealand through Efficient Use of National Information Systems
Cloning and physical characterization of linked lysine genes (LYS4, LYS15) of Saccharomyces cerevisiae
Increased frontal and paralimbic activation following ayahuasca, the pan-amazonian inebriant
Major histocompatibility complex class I-restricted alloreactive CD4(+) T cells
Although it is well established that CD4(+) T cells generally recognize major histocompatibility complex (MHC) class II molecules, MHC class I-reactive CD4(+) T cells have occasionally been reported. Here we describe the isolation and characterization of six MHC class I-reactive CD4(+) T-cell lines, obtained by co-culture of CD4(+) peripheral blood T cells with the MHC class II-negative, transporter associated with antigen processing (TAP)-negative cell line, T2, transfected with human leucocyte antigen (HLA)-B27. Responses were inhibited by the MHC class I-specific monoclonal antibody (mAb), W6/32, demonstrating the direct recognition of MHC class I molecules. In four cases, the restriction element was positively identified as HLA-A2, as responses by these clones were completely inhibited by MA2.1, an HLA-A2-specific mAb. Interestingly, three of the CD4(+) T-cell lines only responded to cells expressing HLA-B27, irrespective of their restricting allele, implicating HLA-B27 as a possible source of peptides presented by the stimulatory MHC class I alleles. In addition, these CD4(+) MHC class I alloreactive T-cell lines could recognize TAP-deficient cells and therefore may have particular clinical relevance to situations where the expression of TAP molecules is decreased, such as viral infection and transformation of cells