9 research outputs found
Singularly Perturbed Monotone Systems and an Application to Double Phosphorylation Cycles
The theory of monotone dynamical systems has been found very useful in the
modeling of some gene, protein, and signaling networks. In monotone systems,
every net feedback loop is positive. On the other hand, negative feedback loops
are important features of many systems, since they are required for adaptation
and precision. This paper shows that, provided that these negative loops act at
a comparatively fast time scale, the main dynamical property of (strongly)
monotone systems, convergence to steady states, is still valid. An application
is worked out to a double-phosphorylation ``futile cycle'' motif which plays a
central role in eukaryotic cell signaling.Comment: 21 pages, 3 figures, corrected typos, references remove
Comparison of novel and conventional methods of trapping ixodid ticks in the southeastern U.S.A.
Identification of a CAPA-PVK [Ixori-PVK] from single cells of the gulf Gulf Coast tick, Amblyomma maculatum
MALDI-TOF/TOF tandem mass spectrometry has been applied to
determine the complete sequence of a CAPA-PVK in the Gulf Coast tick, Amblyomma
maculatum. Single cell analysis allowed the identification of the amino acid sequence
of Ixori-PVK (PALIPFPRV-NH2), a periviscerokinin which had previously been
identified from two other ticks, Ixodes ricinus and Boophilus microplus. The
identification indicates greater conservation of sequence for the CAPA-PVK/CAP2b
family in ticks as compared with insects. Side-chain fragmentation experiments
provided data to distinguish between Leu/Ile ambiguities. The tick CAPA peptide
shows a high sequence homology with other members of the insect periviscerokinin/
CAP2b peptides, which are associated with the regulation of critical physiological
processes such as diuresis. Thus, the identification of this neuropeptide will provide
the experimental basis to better understand regulation of water balance in these
arthropods, providing a potential opportunity to develop neuropeptide-based control
strategies against these livestock pests