A database of number fields

We describe an online database of number fields which accompanies this paper The database centers on complete lists of number fields with prescribed invariants. Our description here focuses on summarizing tables and connections to theoretical issues of current interest.Comment: 25 pages, 1 figur

Stark ladders as tunable far-infrared emitters

A superlattice of GaAs/Ga(1 – x)Al(x)As quantum wells forms a Stark ladder under the influence of a perpendicular electric field. A two level incoherent emitter system, formed by radiative intersubband transitions between adjacent wells, is investigated as a tunable far-infrared radiation source. Intersubband transition rates are calculated at 4, 77, and 300 K for applied fields from 0 to 40 kV cm(–1). It is shown that the quantum efficiency of the radiative emission reaches a maximum at low temperatures for a field of 32 kV cm(–1). Under these conditions the emission wavelength is 38 µm with an estimated power output of 1.1 mW. © 1998 American Institute of Physics

The Toxoplasma gondii plastid replication and repair enzyme complex, PREX

A plastid-like organelle, the apicoplast, is essential to the majority of medically and veterinary important apicomplexan protozoa including Toxoplasma gondii and Plasmodium. The apicoplast contains multiple copies of a 35 kb genome, the replication of which is dependent upon nuclear-encoded proteins that are imported into the organelle. In P. falciparum an unusual multi-functional gene, pfprex, was previously identified and inferred to encode a protein with DNA primase, DNA helicase and DNA polymerase activities. Herein, we report the presence of a prex orthologue in T. gondii. The protein is predicted to have a bi-partite apicoplast targeting sequence similar to that demonstrated on the PfPREX polypeptide, capable of delivering marker proteins to the apicoplast. Unlike the P. falciparum gene that is devoid of introns, the T. gondii prex gene carries 19 introns, which are spliced to produce a contiguous mRNA. Bacterial expression of the polymerase domain reveals the protein to be active. Consistent with the reported absence of a plastid in Cryptosporidium species, in silico analysis of their genomes failed to demonstrate an orthologue of prex. These studies indicate that prex is conserved across the plastid-bearing apicomplexans and may play an important role in the replication of the plastid genome

Molecular basis for resistance of acanthamoeba tubulins to all major classes of antitubulin compounds

Tubulin is essential to eukaryotic cells and is targeted by several antineoplastics, herbicides, and antimicrobials. We demonstrate that Acanthamoeba spp. are resistant to five antimicrotubule compounds, unlike any other eukaryote studied so far. Resistance correlates with critical amino acid differences within the inhibitor binding sites of the tubulin heterodimers

MEXIT: Maximal un-coupling times for stochastic processes

Classical coupling constructions arrange for copies of the \emph{same} Markov process started at two \emph{different} initial states to become equal as soon as possible. In this paper, we consider an alternative coupling framework in which one seeks to arrange for two \emph{different} Markov (or other stochastic) processes to remain equal for as long as possible, when started in the \emph{same} state. We refer to this "un-coupling" or "maximal agreement" construction as \emph{MEXIT}, standing for "maximal exit". After highlighting the importance of un-coupling arguments in a few key statistical and probabilistic settings, we develop an explicit \MEXIT construction for stochastic processes in discrete time with countable state-space. This construction is generalized to random processes on general state-space running in continuous time, and then exemplified by discussion of \MEXIT for Brownian motions with two different constant drifts.Comment: 28 page

Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii

Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period of 12 months when stored at −70°C using dimethyl sulfoxide (DMSO; 5 or 10%) as cryopreservant. As A. castellanii are naturally capable of encystment, studies were undertaken to determine whether induced encystment might improve the viability of organisms under a number of storage conditions. A. castellanii cysts stored in the presence of Mg2+ at 4°C remained viable over the study period, although time to confluence was increased from approximately 8 days to approximately 24 days over the 12-month period. Storage of cysts at −70°C with DMSO (5 or 10%) or 40% glycerol, but not 80% glycerol as cryopreservants increased their viability over the 12-month study period compared with those stored at room temperature. Continued presence of Mg2+ in medium during storage had no adverse effects and generally improved recovery of viable organisms. The present study demonstrates that A. castellanii can be stored as a non-multiplicative form inexpensively, without a need for cryopreservation, for at least 12 months, but viability is increased by storage at −70°C