Mass assembly and AGN activity at z≳1.5z\gtrsim1.5 in the dense environment of XDCPJ0044.0-2033

XDCP0044.0-2033 is the most massive galaxy cluster known at z>1.5 and its core shows a high density of galaxies which are experiencing mergers and hosting nuclear activity. We present a multi-wavelength study of a region located 157 kpc from the center of this galaxy cluster, for which we have photometric and spectroscopic multi-wavelength observations (high resolution HST images in F105W, F140W and F160W bands, NIR KMOS data in H and YJ bands and Chandra ACIS-S X-ray data). Our main goal is to investigate the environmental effects acting on the galaxies inhabiting this high density region. We find that the analyzed region hosts at least nine different sources, six of them confirmed to be cluster members within a narrow redshift range 1.5728<z<1.5762. These sources form two different complexes at a projected distance of $\sim$13 kpc, which are undergoing merging on an estimated timescale off 10-30 Myr. One of the sources shows the presence of a broad H alpha emission line and is classified as Type 1 AGN. This AGN is associated to an X-ray point-like source, whose emission appears moderately obscured (with intrinsic absorption $N_{H} \sim 10^{22} cm^{-2}$) and hosts a relatively massive black hole with mass $M_{BH} \sim 10^{7} M_{\odot}$, which is accreting with an Eddington ratio of $\sim$0.2. We conclude that the analyzed region is consistent with being the formation site of a secondary BCG. These findings, together with an in-depth analysis the X-ray morphology of the cluster, suggest a merging scenario of the entire cluster, with two massive halos both harbouring two rapidly evolving BCGs on the verge of being assembled. Our results are also consistent with the scenario in which the AGN phase in member galaxies is triggered by gas-rich mergers, playing a relevant role in the formation of the red sequence of elliptical galaxies observed in the center of local galaxy clusters

AGN-Host Interaction in IC 5063. I. Large-scale X-Ray Morphology and Spectral Analysis

We report the analysis of the deep (270 ks) X-ray Chandra data of one of the most radio-loud, Seyfert 2 galaxies in the nearby Universe (z=0.01135), IC 5063. The alignment of the radio structure with the galactic disk and ionized bi-cone, enables us to study the effects of both radio jet and nuclear irradiation on the interstellar medium (ISM). The nuclear and bi-cone spectra suggest a low photoionization phase mixed with a more ionized or thermal gas component, while the cross-cone spectrum is dominated by shocked and collisionally ionized gas emission. The clumpy morphology of the soft (<3 keV) X-ray emission along the jet trails, and the large (~2.4 kpc) filamentary structure perpendicular to the radio jets at softer energies (<1.5 keV), suggest a large contribution of the jet-ISM interaction to the circumnuclear gas emission. The hard X-ray continuum (>3 keV) and the Fe K-alpha 6.4 keV emission are both extended to kpc size along the bi-cone direction, suggesting an interaction of nuclear photons with dense clouds in the galaxy disk, as observed in other Compton Thick (CT) active nuclei. The north-west cone spectrum also exhibits an Fe XXV emission line, which appears spatially extended and spatially correlated with the most intense radio hot-spot, suggesting jet-ISM interaction.Comment: 28 pages, 10 figures and 3 tables (with 10 more figures and 3 more tables in the Appendix), submitted to ApJ on May 24, 202

A Grafting Strategy for the Design of Improved G-Quadruplex Aptamers and High-Activity DNAzymes

Nucleic acid aptamers are generally obtained by in vitro selection. Some have G-rich consensus sequences with ability to fold into the four-stranded structures known as G-quadruplexes. A few G-quadruplex aptamers have proven to bind hemin to form a new class of DNAzyme with the peroxidase-like activity, which can be significantly promoted by appending an appropriate base-pairing duplex onto the G-quadruplex structures of aptamers. Knowing the structural role of base pairing, here we introduce a novel grafting strategy for the design of improved G-quadruplex aptamers and high-activity DNAzymes. To demonstrate this strategy, three existing G-quadruplex aptamers are chosen as the first generation. A base-pairing DNA duplex is grafted onto the G-quadruplex motif of the first generation aptamers. Consequently, three new aptamers with the quadruplex/duplex DNA structures are produced as the second generation. The hemin-binding affinities and DNAzyme functions of the second generation aptamers are characterized and compared with the first generation. The results indicate three G-quadruplex aptamers obtained by the grafting strategy have more excellent properties than the corresponding original aptamers. Our findings suggest that, if the structures and functions of existing aptamers are thoroughly known, the grafting strategy can be facilely utilized to improve the aptamer properties and thereby producing better next-generation aptamers. This provides a simple but effective approach to the design of nucleic acid aptamers and DNAzymes

Advantages of using non-isothermal bioreactors for the enzymatic synthesis of antibiotics: the penicillin G acylase as enzyme model

A new hydrophobic and catalytic membrane was prepared by immobilizing Penicillin G acylase (PGA, EC.3.5.1.11) from E. coli on a nylon membrane, chemically grafted with butylmethacrylate (BMA). Hexamethylenediamine (HMDA) and glutaraldehyde (Glu) were used as a spacer and coupling agent, respectively. PGA was used for the enzymatic synthesis of cephalexin, using D(-)-phenylglycine methyl ester (PGME) and 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) as substrates. Several factors affecting this reaction, such as pH, temperature, and concentrations of substrates were investigated. The results indicated good enzyme-binding efficiency of the pre-treated membrane, and an increased stability of the immobilized PGA towards pH and temperature. Calculation of the activation energies showed that cephalexin production by the immobilized biocatalyst was limited by diffusion, resulting in a decrease of enzyme activity and substrate affinity. Temperature gradients were employed as a way to reduce the effects of diffusion limitation. Cephalexin was found to linearly increase with the applied temperature gradient. A temperature difference of about 3°C across the catalytic membrane resulted into a cephalexin synthesis increase of 100 ith a 50␛eduction of the production times. The advantage of using non-isothermal bioreactors in biotechnological processes, including pharmaceutical applications, is also discussed

Solitary skin metastasis from papillary thyroid carcinoma

Although cutaneous lesions in patients with history of thyroid cancer are rare, the present report describes the appearance of a small skin metastasis that grew in situ over about 20 years from the initial diagnosis without any local or distant metastases