Treatment with the immunomodulator FTY720 does not promote spontaneous bacterial infections after experimental stroke in mice

Background: FTY720, an immunomodulator derived from a fungal metabolite which reduces circulating lymphocyte counts by increasing the homing of lymphocytes to the lymph nodes has recently gained interest in stroke research. The aim of this study was to evaluate the protective efficacy of FTY720 in cerebral ischemia in two different application paradigms and to gather first data on the effect of FTY720 on the rate of spontaneous bacterial infections in experimental stroke. Methods: Middle cerebral artery occlusion (MCAO) in C57BL/6 mice (strain J, groups of 10 animals) was performed with two different durations of ischemia (90 min and 3 h) and FTY720 was applied 2 h after vessel occlusion to study the impact of reperfusion on the protective potency of FTY720. Lesion size was determined by TTC staining. Mice treated with FTY720 or vehicle were sacrificed 48 h after 90 min MCAO to determine the bacterial burden in lung and blood. Results: FTY720 1 mg/kg significantly reduced ischemic lesion size when administered 2 h after the onset of MCAO for 3 h (45.4 +/- 22.7 mm3 vs. 84.7 +/- 23.6 mm3 in control mice, p = 0.001) and also when administered after reperfusion, 2 h after the onset of MCAO for 90 min (31.1 +/- 28.49 mm3 vs. 69.6 +/- 27.2 mm3 in control mice, p = 0.013). Bacterial burden of lung homogenates 48 h after stroke did not increase in the group treated with the immunomodulator FTY720 while there was no spontaneous bacteremia 48 h after MCAO in treated and untreated animals. Conclusions: Our results corroborate the experimental evidence of the protective effect of FTY720 seen in different rodent stroke models. Interestingly, we found no increase in bacterial lung infections even though FTY720 strongly reduces the number of circulating leukocytes

Effect of lutein and antioxidant dietary supplementation on contrast sensitivity in age-related macular disease:A randomized controlled trial

Objective: The aim of the study is to determine the effect of lutein combined with vitamin and mineral supplementation on contrast sensitivity in people with age-related macular disease (ARMD). Design: A prospective, 9-month, double-masked randomized controlled trial. Setting: Aston University, Birmingham, UK and a UK optometric clinical practice. Subjects: Age-related maculopathy (ARM) and atrophic age-related macular degeneration (AMD) participants were randomized (using a random number generator) to either placebo (n = 10) or active (n=15) groups. Three of the placebo group and two of the active group dropped out. Interventions: The active group supplemented daily with 6 mg lutein combined with vitamins and minerals. The outcome measure was contrast sensitivity (CS) measured using the Pelli-Robson chart, for which the study had 80% power at the 5% significance level to detect a change of 0.3log units. Results: The CS score increased by 0.07 ± 0.07 and decreased by 0.02 ± 0.18 log units for the placebo and active groups, respectively. The difference between these values is not statistically significant (z = 0.903, P = 0.376). Conclusion: The results suggest that 6 mg of lutein supplementation in combination with other antioxidants is not beneficial for this group. Further work is required to establish optimum dosage levels

Prevention of age-related macular degeneration

Age-related macular degeneration (AMD) is one of the leading causes of blindness in the developed world. Although effective treatment modalities such as anti-VEGF treatment have been developed for neovascular AMD, there is still no effective treatment for geographical atrophy, and therefore the most cost-effective management of AMD is to start with prevention. This review looks at current evidence on preventive measures targeted at AMD. Modalities reviewed include (1) nutritional supplements such as the Age-Related Eye Disease Study (AREDS) formula, lutein and zeaxanthin, omega-3 fatty acid, and berry extracts, (2) lifestyle modifications, including smoking and body-mass-index, and (3) filtering sunlight, i.e. sunglasses and blue-blocking intraocular lenses. In summary, the only proven effective preventive measures are stopping smoking and the AREDS formula

Germination Energy, Germination Capacity and Microflora of Allium cepa L. Seeds after RF Plasma Conditioning

This paper presents the results of an experiment on the effect of the cold plasma (He+O2 or He+Air) pre-sowing stimulation of seeds of the Wolska cultivar of onion on the process of their germination. Four groups of seeds characterized by different exposure times (60, 120, 240 and 480 s) were used. Untreated seeds were used as a control. The distance between the electrode and the tested material was 50 mm. Pre-sowing plasma stimulation improved germination parameters such as germination capacity and germination energy for all the tested groups relative to the control. The highest fractions of germinated seeds were observed for an exposure time of 120 s. Analysis of the data showed a statistically significant impact of RF plasma on the seed germination parameters of the onion. SEM analysis showed that the interaction with plasma produced tension in the cells, leading to a change in their shape. No visible damage to the onion seed cells was observed, apart from the effect of depletion of the upper wax layer. The best influence on pathogenic fungi was when the group of seeds underwent 240 and 480 s of exposure to plasma fumigation, especially using the He+Air RF plasma jet

Pulsed electromagnetic field: an organic compatible method to promote plant growth and yield in two corn types

Pre-sowing treatment of pulsed electromagnetic fields was used in corn seeds, in both indoor and outdoor conditions, in order to investigate the effect on plant growth and yield. The results of this research showed that pulsed electromagnetic fields can enhance plant characteristics, both under controlled environmental conditions and uncontrolled field conditions. The two varieties responded differently in the duration of magnetic field. Seeds were treated for 0, 15, 30, and 45 min with pulsed electromagnetic field (MF-0, MF-15, MF-30, and MF-45). Common corn variety performed better results in MF-30 treatment, while sweet corn variety performed better in MF-45 treatment. Magnetic field improved germination percentage, vigor, chlorophyll content, leaf area, plant fresh and dry weight, and finally yields. In the very interesting measurement of yield, seeds that have been exposed to magnetic field for 30 and 45 min have been found to perform the best results with no statistical differences among them. Another interesting finding was in root dry weight measurements, where magnetic field has a negative impact in MF-30 treatment in both hybrids, however without affecting other measurements. Enhancements on plant characteristics with economic impact on producer's income could be the future of a modern, organic, and sustainable agriculture