3 research outputs found
Analysis of cow dung microbiota—A metagenomic approach
372-378Cow dung is being used from ancient times in
agriculture as it has a significant role in plant growth promotion and plant
protection. It is also being used in various religious practices as a purifier.
Since only a small fraction of the total microbial diversity can be recovered
by culturable methods, a culture independent 16S rDNA approach was taken up for
more detailed analysis of cow dung microbiota. Total community DNA was extracted from fresh dung of Brown-Swiss
breed and bacterial 16S rRNA genes were subsequently amplified, cloned,
sequenced and deposited in GenBank. Bacteria belonging to the phyla
Bacteroidetes (38.3%), Firmicutes (29.8%), Proteobacteria (21.3%) and
Verrucomicrobia (2%) were identified. Bacteroidetes clones included the genera Bacteroides, <i style="mso-bidi-font-style:
normal">Alistipes and Paludibacter;
while Clostridium, Ruminococcus, <i style="mso-bidi-font-style:
normal">Anaerovorax and Bacillus
were predominant in Firmicutes. α- and γ-proteobacterial genera included Acinetobacter, <i style="mso-bidi-font-style:
normal">Pseudomonas, Rheinheimera,
Stenotrophomonas and Rhodobacter. The Verrucomicrobial clone
showed high similarity to Akkermansia.
Unculturable bacteria constituted 83.3% in the phylum Bacteroidetes and 87.5%
in Firmicutes. All clones under phylum Proteobacteria were culturable bacteria.
Eight per cent of the clone library represented previously uncharacterized and
unidentified bacteria