Analisis Faktor Yang Mempengaruhi Intensi Pembelian Hijau Pada Green Cosmetic

The current increase public awareness of the environment against environmental degradation , environmental awareness is also affecting people's habits as consumers . People tend to want products that are environmentally friendly . These conditions resulted in the company adapting environmental problems . One of the concepts that currently can be adapted by the company is green marketing . Companies that create products are green can pave the way for manufacturers to enter the market , especially green product market . This study used a survey method . Factors examined include factors perceived product price and quality ( PQ ) , organization 's green image ( OGI ) , environmental concerns ( EC ) , and environmental knowledge ( EK ) against green purchase intention ( GPI ) . The research found that before dimoderating by PQ all independent factors have a significant influence on purchase intentions green . After dimoderating by PQ all independent factors did not have a significant effect on purchase intentions green because consumers are sensitive to price causes consumer intentions to buy the product is reduced . Businessmen are advised to consider the factors in order to improve the cosmetic green business

Gene-Trap Mutagenesis Identifies Mammalian Genes Contributing to Intoxication by Clostridium perfringens ε-Toxin

The Clostridium perfringens ε-toxin is an extremely potent toxin associated with lethal toxemias in domesticated ruminants and may be toxic to humans. Intoxication results in fluid accumulation in various tissues, most notably in the brain and kidneys. Previous studies suggest that the toxin is a pore-forming toxin, leading to dysregulated ion homeostasis and ultimately cell death. However, mammalian host factors that likely contribute to ε-toxin-induced cytotoxicity are poorly understood. A library of insertional mutant Madin Darby canine kidney (MDCK) cells, which are highly susceptible to the lethal affects of ε-toxin, was used to select clones of cells resistant to ε-toxin-induced cytotoxicity. The genes mutated in 9 surviving resistant cell clones were identified. We focused additional experiments on one of the identified genes as a means of validating the experimental approach. Gene expression microarray analysis revealed that one of the identified genes, hepatitis A virus cellular receptor 1 (HAVCR1, KIM-1, TIM1), is more abundantly expressed in human kidney cell lines than it is expressed in human cells known to be resistant to ε-toxin. One human kidney cell line, ACHN, was found to be sensitive to the toxin and expresses a larger isoform of the HAVCR1 protein than the HAVCR1 protein expressed by other, toxin-resistant human kidney cell lines. RNA interference studies in MDCK and in ACHN cells confirmed that HAVCR1 contributes to ε-toxin-induced cytotoxicity. Additionally, ε-toxin was shown to bind to HAVCR1 in vitro. The results of this study indicate that HAVCR1 and the other genes identified through the use of gene-trap mutagenesis and RNA interference strategies represent important targets for investigation of the process by which ε-toxin induces cell death and new targets for potential therapeutic intervention

A modified nonlinear spectral Galerkin method for the equations of motion arising in the Kelvin-Voigt fluids

In this paper, a variant of nonlinear Galerkin method is proposed and analysed for equations of motions arising in a Kelvin-Voigt model of viscoelastic fluids in a bounded spatial domain in IRd (d = 2, 3). Some new a priori bounds are obtained for the exact solution when the forcing function is independent of time or belongs to L-infinity in time. As a consequence, existence of a global attractor is shown. For the spectral Galerkin scheme, existence of a unique discrete solution to the semidiscrete scheme is proved and again existence of a discrete global attractor is established. Further, optimal error estimate in L-infinity(L-2) and L-infinity(H-0(1))-norms are proved. Finally, a modified nonlinear Galerkin method is developed and optimal error bounds are derived. It is, further, shown that error estimates for both schemes are valid uniformly in time under uniqueness condition

Benzothiazole hydrazones of furylbenzamides preferentially stabilize c-MYC and c-KIT1 promoter G-quadruplex DNAs

The stabilization of G-quadruplex DNA structures by using small molecule ligands having simple structural scaffolds has the potential to be harnessed for developing next generation anticancer agents. Because of the structural diversity of G-quadruplexes, it is challenging to design stabilizing ligands, which can specifically bind to a particular quadruplex topology. To address this, herein, we report the design and synthesis of three benzothiazole hydrazones of furylbenzamides having different side chains (ligands 1, 2 and 3), which show preferential stabilization of promoter quadruplex DNAs (c-MYC and c-KIT1) having parallel topologies over telomeric and duplex DNAs. The CD melting study revealed that all the ligands, in particular ligand 2, exhibit higher stabilization toward parallel promoter quadruplexes (Delta T-m = 10-15 degrees C) as compared to antiparallel promoter quadruplex (h-RAS1), telomeric quadruplex and duplex DNAs (Delta T-m = 0-3 degrees C). FID assay and fluorimetric titration results also reveal the preferential binding of ligands toward c-MYC and c-KIT1 promoter quadruplex DNAs over telomeric and duplex DNAs. Validating these results further, Taq DNA polymerase stop assay showed IC50 similar to 6.4 mu M for ligand 2 with the c-MYC DNA template, whereas the same for the telomeric DNA template was found to be >200 mu M. Molecular modeling and dynamics studies demonstrated a 1 : 1 binding stoichiometry in which stacking and electrostatic interactions play important roles in stabilizing the c-MYC G-quadruplex structure. Taken together, the results presented here provide new insights into the design of structurally simple scaffolds for the preferential stabilization of a particular G-quadruplex topology