780 research outputs found
Site-Directed Spin Labeling and Electron Paramagnetic Resonance (EPR) Spectroscopy: A Versatile Tool to Study Protein-Protein Interactions
Millimetre-VLBI Monitoring of AGN with Sub-milliarcsecond Resolution
Global millimetre VLBI allows detailed studies of the most central jet
regions of AGN with unprecedent spatial resolution of a few 100-1000
Schwartzschild radii to be made. Study of these regions will help to answer the
question how the highly relativistic AGN jets are launched and collimated.
Since the early 1990s, bright mm-sources have been observed with global 3 mm
VLBI. Here we present new images from an ongoing systematic analysis of the
available observations. In particular, we focus on the structure and structural
evolution of the best observed AGN jets, taking 3C 454.3 as a characteristic
example. This core-dominated and highly variable quasar shows a complex
morphology with individual jet components accelerating superluminally towards
the outer structure. We briefly discuss the X-ray properties of 3C 454.3 and
present its radio- to X-ray large-scale brightness distribution.Comment: 4 pages, 4 figures, Proceedings of the 7th EVN Symposium held in
Toledo, Spain in October 2004, needs evn2004.cl
Modeling of the Super-Eddington Phase for Classical Novae: Five IUE Novae
We present a light curve model for the super-Eddington luminosity phase of
five classical novae observed with IUE. Optical and UV light curves are
calculated based on the optically thick wind theory with a reduced effective
opacity for a porous atmosphere. Fitting a model light curve with the UV 1455
\AA light curve, we determine the white dwarf mass and distance to be (1.3
M_sun, 4.4 kpc) for V693 CrA, (1.05 M_sun, 1.8 kpc) for V1974 Cyg, (0.95 M_sun,
4.1 kpc) for V1668 Cyg, (1.0 M_sun, 2.1 kpc) for V351 Pup, and (1.0 M_sun, 4.3
kpc) for OS And.Comment: 9 pages including 8 figures, to appear in the Astrophysical Journa
9286 Stars: An Agglomeration of Stellar Polarization Catalogs
This is a revision. The revisions are minor. The new version of the catalog
should be used in preference to the old. The most serious error in the older
version was that was incorrect, being sometimes far too large,
for Reiz and Franco entries; the correct values are all zero for that
reference.
We present an agglomeration of stellar polarization catalogs with results for
9286 stars. We have endeavored to eliminate errors, provide accurate
(arcsecond) positions, sensibly weight multiple observations of the same star,
and provide reasonable distances. This catalog is included as an ASCII file
(catalog.txt) in the source of this submission.Comment: The most serious error in the older version was that
was incorrect, being sometimes far too large, for Reiz and Franco entries;
the correct values are all zero for that reference. 11 pages, no figures.
Accepted for Astronomical Journal. Catalog also available as an ASCII file by
anonymous FTP from ftp://vermi.berkeley.edu/pub/polcat/p14.ou
Dynamics of Line-Driven Winds from Disks in Cataclysmic Variables. I. Solution Topology and Wind Geometry
We analyze the dynamics of 2-D stationary, line-driven winds from accretion
disks in cataclysmic variable stars. The driving force is that of line
radiation pressure, in the formalism developed by Castor, Abbott & Klein for O
stars. Our main assumption is that wind helical streamlines lie on straight
cones. We find that the Euler equation for the disk wind has two eigenvalues,
the mass loss rate and the flow tilt angle with the disk. Both are calculated
self-consistently. The wind is characterized by two distinct regions, an outer
wind launched beyond four white dwarf radii from the rotation axis, and an
inner wind launched within this radius. The inner wind is very steep, up to 80
degrees with the disk plane, while the outer wind has a typical tilt of 60
degrees. In both cases the ray dispersion is small. We, therefore, confirm the
bi-conical geometry of disk winds as suggested by observations and kinematical
modeling. The wind collimation angle appears to be robust and depends only on
the disk temperature stratification. The flow critical points lie high above
the disk for the inner wind, but close to the disk photosphere for the outer
wind. Comparison with existing kinematical and dynamical models is provided.
Mass loss rates from the disk as well as wind velocity laws are discussed in a
subsequent paper.Comment: 21 pages, 10 Postscript figures; available also from
http://www.pa.uky.edu/~shlosman/publ.html. Astrophysical Journal, submitte
Light-Induced Switching of HAMP Domain Conformation and Dynamics Revealed by Time-Resolved EPR Spectroscopy
The crystal structure of the C-terminal domain of the salmonella enterica pduo protein: An old fold with a new heme-binding mode
The two-domain protein PduO, involved in 1,2-propanediol utilization in the pathogenic Gram-negative bacterium Salmonella enterica is an ATP:Cob(I)alamin adenosyltransferase, but this is a function of the N-terminal domain alone. The role of its C-terminal domain (PduOC) is, however, unknown. In this study, comparative growth assays with a set of Salmonella mutant strains showed that this domain is necessary for effective in vivo catabolism of 1,2-propanediol. It was also shown that isolated, recombinantly-expressed PduOC binds heme in vivo. The structure of PduOC co-crystallized with heme was solved (1.9 \uc5 resolution) showing an octameric assembly with four heme moieities. The four heme groups are highly solvent-exposed and the heme iron is hexa-coordinated with bis-His ligation by histidines from different monomers. Static light scattering confirmed the octameric assembly in solution, but a mutation of the heme-coordinating histidine caused dissociation into dimers. Isothermal titration calorimetry using the PduOC apoprotein showed strong heme binding (Kd = 1.6
7 10 127 M). Biochemical experiments showed that the absence of the C-terminal domain in PduO did not affect adenosyltransferase activity in vitro. The evidence suggests that PduOC:heme plays an important role in the set of cobalamin transformations required for effective catabolism of 1,2-propanediol. Salmonella PduO is one of the rare proteins which binds the redox-active metabolites heme and cobalamin, and the heme-binding mode of the C-terminal domain differs from that in other members of this protein family
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