Epidemiological data of falciparum malaria in Ado-Odo/Ota, Southwest Ogun State, Nigeria

In this data article, Blood and corresponding saliva samples from subjects presenting with fever and parasetaemia Z2000 were obtained from selected hospitals in Ado-Odo/Ota, Ogun State over a period of two years and analyzed using Polymerase chain reaction-Restriction fragment Length Polymorphism (PCR/Nested PCR-RFLP) to detect genetic mutations of Plasmodium falciparum chloroquine resistance transporter (Pfcrt), Plasmodium falciparum multidrugs resistance (Pfmdr1) and non-synonymous Pkelch (pk13) mutated genes. The study confirmed the presence of resistance genes in the blood and saliva samples collected from the study sit

In-vivo Antiplasmodial Activity of Crude n-hexane and Ethanolic Extracts of Moringa oleifera (LAM.) Seeds on Plasmodium berghei

Studies were carried out to determine the antiplasmodial activity of crude n-hexane and ethanolic seed extracts of Moringa oleifera using cold extraction method. Twenty-four albino mice (Mus musculus) induced intraperitoneally with chloroquine sensitive Plasmodium berghei strain were divided into 4 groups and treated at three concentrations viz: 50, 100 and 200 ml/kg. Positive control was set up with chloroquine diphosphate while negative control was set up with olive oil. The mice models were treated for 72 h. For the ethanolic extract, a parasite inhibition rate of 61% was observed at concentration 50 ml/kg, 65% at concentration 100 ml/kg and 100% at concentration 200 ml/kg in day 3 after treatment. In n-hexane extract of the seeds of M. oleifera, plasmodial inhibition rate of 61% was observed at concentration 50 ml/kg, 70% at concentration 100 ml/kg and 97% at concentration 200 ml/kg after treatment for 72 h. A 100% inhibition rate was observed for mice treated with 25 mg/kg of standard chloroquine diphosphate after day 3 of treatment while parasitaemia increased from 48 on day 0 to 86 after day 3 for mice treated with olive oil. Overall, crude ethanolic extract of M. oleifera seed showed higher parasite inhibition activity than the crude n-hexane extrac

COVID-19 Pandemic: Perception, Practices and Preparedness in Nigeria

Since Coronavirus disease 19 (COVID-19) pandemic was declared a public health emergency of international concern by the World Health Organization (WHO) on the 30th of January, 2020. Nigeria, with 343 cases and 10 deaths as at April 14, 2020 is classified as one of the countries at high risk of importation of the disease from China. The ability to limit and control local transmission after importation depends on the application and execution of strict measures of detection, prevention and control. The initial response of some percentage of the population was of doubt due to the ignorance of the far-reaching effect of the virus. More than 1,700 leaders of religious groups and communities in all 36 States and FCT were therefore sensitized to increase awareness level and consequences of COVID-19 among the populace. Major response activities were initiated before the first case was reported and were upgraded within weeks after the number of cases began to rise. Based on previous experience of perception, and awareness of other viral disease outbreaks, COVID-19 infection prevention and control interventions recommended by WHO are yet to be fully entrenched in the Nigerian public health system in order to reduce the general risk of contracting SARS-CoV-2 from infected individuals. There is therefore the need to execute strict measures of detection, prevention and control and drive compliance with the Nigeria Centre for Disease Control (NCDC) and WHO guidelines in Nigeria

Occurrence of Fluoride and some Heavy Metals in Groundwater from Shallow Aquifers Near Ogbomosho, North-central Nigeria

Groundwater is one of the most important natural resources that when contaminated by either natural or anthropogenic means is difficult and expensive to clean-up. Fluorosis is a disease affecting the bone and teeth of humans due to excessive intake of fluoride either through water or food. Heavy metal pollution is a burning environmental issue due their toxic, persistent and bio-accumulative nature. The present study evaluates the presence of fluoride and some heavy metals in groundwater from shallow aquifers around Ogbomosho, north-central Nigeria and the result of investigation confirmed their enrichment. The fluoride concentration ranged between 1.35mg/l to 2.75mg/l with a mean value of 2.18mg/l as against the recommended value of 1.50mg/l. This is an indication that continuous use of water from this area may result to colouration of the teeth and deformation of the bone among the people especially children since they are the most vulnerable. High fluoride content in groundwater can be attributed to the continuous water-rock interaction during the process of percolation with fluoride-bearing country rocks under arid, low precipitation, and high evapotranspiration conditions. The study has established that the fluoride-rich groundwater as well as heavy metal contamination in the area may have emanated from geochemical processes of dissolution and weathering of the granite aquifers in the area. The heavy metal enrichment is in the order of: Ni > Mn > Cr > Cd > Fe > Cu > Zn. These findings suggest that the enrichment of the groundwater system is geogenic and related to the local geology of the area. It is recommended that people living in the coarse grained porphyritic biotite granite dominated area should discontinue the use of groundwater for domestic and drinking purposes in order not to experience fluorosis in future. Environmentally friendly techniques such as phyto-remediation and bio-remediation should be employed to monitor and control fluoride and heavy metal content in the groundwater system. Keywords: Groundwater Quality, Fluorosis, Heavy metals contamination, Shallow Aquifers Ogbomosho and North-central Nigeri

Fecal carriage and phylodiversity of communityacquired bla TEM Enteric bacilli in Southwest Nigeria

Purpose: Increasing rates of clonal spread of fecal blaTEM bacilli remains a huge concern to the community health with resultant high morbidity. The fecal carriage and clonal diversity of blaTEM within the communities in Southwest Nigeria were surveyed. Materials and methods: Enteric bacilli obtained from fresh fecal samples randomly collected from community residents were biotyped and profiled for antibiotic susceptibility. Resistant strains were typed for beta-lactamase, extended-spectrum beta-lactamases (ESBL), AmpC and carbapenemase production while the R-plasmid carriage was detected and mating activities were examined. The presence of blaTEM gene was assayed by PCR and its phylodiversity determined with 16sRNA genomic profiling. Results: Escherichia coli have the highest (28.6%) occurrence rate and Pseudomonas aeruginosa (20.5%) showing significant resistance to beta-lactamase inhibitors (ampicillin, cefuroxime and cefotaxime), and high-level multidrug resistance of more than 15.2% rate to ampicillin, cefuroxime, ceftazidime, tetracycline and imipenem. E. coli and Klebsiella oxytoca, are the highest beta-lactamase, ESBL and AmpC producers encoded with high molecular weight R-plasmid (>11.0 kbp) and significant rate of conjugation and transformational activities. Only 2/14, 1/13 and 1/6 ESBL-type of E. coli, K. oxytoca and Enterobacter cloaca, expressed blaTEM gene, clustering into five different phylodiverse groups with close genomic relatedness with other bacilli. Conclusion: This is an indication of clonal dissemination of ESBL blaTEM encoded enteric bacilli having high phylodiverse characteristics through fecal carriage in the Nigerian community which requires public health education, food and environmental hygiene for its preventio

Epidemiology of Plasmodium falciparum infection and drug resistance markers in Ota Area, Southwestern Nigeria

Purpose: Effective routine monitoring and surveillance of parasite genes is a necessary strategy in the control of parasites’ resistance to antimalarial drugs, according to the WHO’s recommendation. This cross-sectional study therefore aimed at carrying out an epidemiological analysis on malaria incidence in Ado-Odo/Ota, Ogun State. Patients and methods: Blood and corresponding saliva samples were collected from 1,243 subjects of all ages and sex presenting with fever and a parasitemia level ≥2,000 between September 2016 and March 2018. Samples were collected from selected health facilities in the study area of Ogun state to establish the prevalence of falciparum malaria and determine resistance genes harbored by the parasites. The overall prevalence of falciparum malaria in the study site by microscopic examination was 45.86%. The highest incidence of 57.42% was recorded among male subjects. Point mutations of K76T and N86Y in the Pfcrt and pfmdr-1 genes, as well as non-synonymous mutations in Pfk13 genes, were screened for and sequenced for further analysis. Results: Pfcrt was detectable in 57.42% of blood and 51.02% of saliva samples, respectively. About 34.78% of the subjects that were confirmed microscopically harbored the Pfmdr-1 mutated gene while 26.67% of the saliva samples revealed Pfmdr-1. Epidemiological studies identified the presence of wild-type Pfk13 genes in 21.84% of blood and 44.44% of saliva samples correspondingly. For each of the genes evaluated, saliva portrayed great diagnostic performance when compared with blood. Conclusion: Findings from this study have established the prevalence of malaria and the resistance pattern of P. falciparum in the study area. The findings may help in formulating drug policies and suggest the use of saliva as a noninvasive point-of-care method of diagnosing malaria potentially deployable to rural endemic areas

Purification of Cellulase obtained from Tomato fruits (Lycopersicon lycopersicum (L.) Karst) deteriorated by Aspergillus Flavus Linn.

Tomato fruits infected by Aspergillus flavus Linn produced proteins with cellulolytic activity. The enzyme was partially purified by Ammonium Sulphate Precipitation, Gel filtration and ionexchange chromatography. Three peaks of absorption A, B and C were obtained. Peak B had Cellulase activity with molecular weight of approximately 30,200 Daltons while Peaks A and C lacked Cellulase activity. Elution of components of Peak B on CM Sephadex C-25 produced four peaks of absorption designated Ba, Bb, Bc and Bd. Only components of Peaks Bb and Bc possessed Cellulase activity. Purification folds of approximately 80 and 81 were obtained for components of Peaks Bb and Bc respectively for Cellulase of A. flavus. The apparent Km values for the hydrolysis of carboxymethylcellulose by A.flavus Cellulase fractions, Bb and Bc were approximately 16.7 and 15.4mg/ml respectively. The partially purified enzyme preparations obtained from A.flavus during the deterioration of tomato fruits caused tissue maceration and cellular death. This result can be very useful in splitting and solubilization of pectic substances and pathogenicity

Molecular Detection of ESBLs, TEM, SHV, and CTX‐M in Clinical Pseudomonas aeruginosa Isolates in Ogun State

This study sought to detect the Class A extended‐spectrum beta‐lactamases (ESBLs) present in Pseudomonas aeruginosa from clinical samples using molecular methods. Twenty‐seven P. aeruginosa isolates were characterized among one hundred and fifty clinical samples obtained from three major hospitals in Ogun State. Twenty‐five isolates were found to be ESBL producers upon phenotypic screening. DNA was extracted using Zymo DNA extraction kit. Polymerase chain reaction was used to amplify the ESBL genes using specific primers for the CTX‐M, SHV, and TEM genes. Agarose gel electrophoresis was used to resolve the amplicons, and they were visualized with a UV transilluminator, 64% (16 isolates) were found positive for TEM, 52 and (13 isolates) for SHV, and 44% (11 isolates) for CTX‐M. Some isolates were found positive for two or more of the screened genes. This research identifies the need for surveillance of ESBL producers within Ogun state

Antibiotic Resistance Status of Pseudomonas aeruginosa in Clinical Isolates in Ogun State

Pathogenic Gram-negative bacteria mostly produce extended-spectrum beta-lactamases (ESBLs), a feature that confers resistance to some newer generations of antibiotics. The study was aimed at evaluating the antibiotic resistance status of Pseudomonas aeruginosa isolates collected from September to November 2020 from tertiary care hospitals in Ogun State, Nigeria. One hundred fifty isolates from clinical samples (high vaginal swab, wound, urine, ear, blood) were obtained, and their susceptibility pattern was determined against eight antibiotics. Out of the 150 samples, 27 P. aeruginosa were identified. Anti-microbial susceptibility testing was carried out on the isolates using the modified Kirby-Bauer disc diffusion method, and ESBL production was detected phenotypically. The mean age group of the patients was 29.56 years. Gentamicin from the aminoglycoside class showed best activity (74.1%), and ampicillin and penicillin showed little to no activity (100% and 96.3%, respectively). Cephalosporins, ceftazidime and cefuroxime were 100% resistant, while the fluoroquinolones Ciprofloxacin and Ofloxacin had a mean resistance of 40.7%. The moderate active antibiotic was streptomycin (44.4% susceptible). The poor monitoring of antibiotic usage, the abuse of their availability and the acquisition of resistance elements by organisms in hospitals and the community may be the causes of high antibiotic resistanc