In this data article, Blood and corresponding saliva samples from subjects presenting with fever and parasetaemia Z2000 were obtained from selected hospitals in Ado-Odo/Ota, Ogun State over a period of two years and analyzed using Polymerase chain reaction-Restriction fragment Length Polymorphism (PCR/Nested PCR-RFLP) to detect genetic mutations of Plasmodium falciparum

chloroquine resistance transporter (Pfcrt), Plasmodium falciparum multidrugs resistance (Pfmdr1) and non-synonymous Pkelch (pk13) mutated genes. The study confirmed the presence of resistance genes in the blood and saliva samples collected from the study sit

Arogundade, D.

Darby, P.

Diji-Geske, Ruth I.

Fadinad, Irawo

Olasehinde, G. I

English

Covenant University Repository

Contents lists available at ScienceDirectData in BriefData in Brief 19 (2018) 1398–1402SMTHDhttps://2352-34(http://cAbbren CorrE-mgrace.oldamolajournal homepage: www.elsevier.com/locate/dibData ArticleEpidemiological data of falciparum malariain Ado-Odo/Ota, Southwest Ogun State, NigeriaI.Ruth Diji-geske, I.Grace Olasehinde n, Irawo Fadinad,Damola Arogundade, Precious DarbyDepartment of Biological Sciences, Covenant University, Ota, Nigeriaa r t i c l e i n f oArticle history:Received 4 April 2018Received in revised form10 May 2018Accepted 5 June 2018Available online 9 June 2018Keywords:MalariaPrevalencePlasmodium falciparumResistance genesdoi.org/10.1016/j.dib.2018.06.00209/& 2018 Published by Elsevier Inc. Thisreativecommons.org/licenses/by/4.0/).viations: PCR, Polymerase chain reaction; Responding author.ail addresses: ruth.diji-geske@stu.cu.edu.ngasehinde@covenantuniversity.edu.cu.ng (I.G.arogundade@stu.cu.edu.ng (D. Arogundadea b s t r a c tIn this data article, Blood and corresponding saliva samples fromsubjects presenting with fever and parasetaemia Z2000 wereobtained from selected hospitals in Ado-Odo/Ota, Ogun State overa period of two years and analyzed using Polymerase chainreaction-Restriction fragment Length Polymorphism (PCR/NestedPCR-RFLP) to detect genetic mutations of Plasmodium falciparumchloroquine resistance transporter (Pfcrt), Plasmodium falciparummultidrugs resistance (Pfmdr1) and non-synonymous Pkelch (pk13)mutated genes. The study confirmed the presence of resistancegenes in the blood and saliva samples collected from the study site.& 2018 Published by Elsevier Inc. This is an open access articleunder the CC BY license(http://creativecommons.org/licenses/by/4.0/).Specification Tableubject area Microbiologyore specific subject area Epidemiology of malariaype of data Tables and graphow data was acquired Sample collection, Microscopy, PCR analysisata format Raw, analyzedis an open access article under the CC BY licenseFLP, Restriction fragment length polymorphism(I.R. Diji-geske),. Olasehinde), fadinad.irawo@stu.cu.edu.ng (I. Fadinad),), precious.darby@stu.cu.edu.ng (P. Darby).EEDDRI.Ruth Diji-geske et al. / Data in Brief 19 (2018) 1398–1402 1399xperimental factors DNA extraction from blood and saliva samples of subjects presentingwith fever and parasitaemia of Z2000 parasites/ul of blood inselected health facilities.xperimental features PCR was used to detect of Plasmodium falciparum parasites andresistance genes.ata source location Medical diagnostics laboratory Covenant University Medical centerand Molecular Research laboratory, Covenant University, Ota, Nigeria.ata accessibility Within this researchelated research article Olasehinde GI, Ojurongbe OO, Fagade EO, Ruchi S, Egwari LO, Ajayi AA,Adeyeba OA. Detection of Molecular Markers of Antimalarial DrugResistance in Plasmodium Falciparum from South-Western Nigeria.Covenant Journal of Physical and Life Sciences; 2014 1(2): 61- 75.Value of the data The data provides an epidemiology on falciparum malaria and resistance genes in the study site. The data set provides researchers with a platform for enhanced studies in the production of non-invasive malaria test kit using saliva. The data establishes the need for improvement of existing drugs/ development of new ones.1. DataThis data article presented and analyzed incidence and prevalence of Plasmodium falciparum andresistance genes (Pfcrt, Pfmdr1 and Pk13) in ADO-Odo/Ota, Ogun State. It also examined the pro-spective of saliva to serve as a non-invasive diagnostic method for malaria diagnosis [1]. This data willencourage pragmatic monitoring and surveillance of falciparum malaria in the research area asrecommended by the WHO's recommendation [2]. It also provides researchers with a platform forenhanced studies in the production of non-invasive malaria test kit using saliva.2. Experimental design, materials and methodsSamples of blood and corresponding saliva from subjects were collected from various hospitals inAdo-Odo Local government area of Ogun State for three years. The study group for the research cutacross sexes of different age groups of patients presenting with fever and parasitaemia of Z2000parasites/ul of blood. Ethical approval for this study was obtained from the Covenant UniversityBiological Sciences Ethical Review Committee (CUBIOSCREC). Informed consent was obtained from allparticipants. Where participant was a minor, consent was obtained from participant's guardian.Parasite identification and infective stage was determined using microscopy study. For molecularstudies, Parasite DNA from blood and saliva was extracted using a genomic DNA extraction kit. NestedPCR-RFLP using the specific primers - Pfcrt, Pfmdr1 and Pk13 gene was carried out [3–5]. Ampliconswere sequenced directly by using each primer for target gene amplification. Data were analysed andpresented as follows; Table 1 shows the incidence of P. falciparum infection in Ado-Odo/Ota Localgovernment area of Ogun State, Nigeria in the year 2015. Table 2 shows the incidence of falciparummalaria in the year 2016. Table 3 shows the incidence of falciparum malaria in the year 2017. Table 4shows the prevalence of falciparum malaria within two years. Table 5 shows the prevalence ofresistance genes. Fig. 1 presents the incidence of P. falciparum malaria infection in males and females.Fig. 2 presents the resistance genes detected in blood and saliva samples. Fig. 3 presents Pfcrt genedetected in blood and exact number of corresponding saliva samples.Table 2Incidence of falciparum malaria in Ado-Odo/Ota, Ogun State (2016).Number of samples collected Number of positive casesAge Male Female Total Male Female Total % Incidencer5 8 15 23 5 8 13 56.5210–20 39 35 74 31 21 52 70.27Z20 38 30 68 14 23 37 54.41Total 85 80 165 50 52 102 61.82Table 3Incidence of falciparum malaria in Ado-Odo/Ota, Ogun State (2017).Number of samples collected Number of positive casesAge Male Female Total Male Female Total % Incidencer5 17 9 26 7 5 12 46.1510–20 41 47 88 12 18 30 34.09Z20 27 41 68 8 12 20 29.41Total 85 97 182 27 35 62 34.07Table 4Prevalence of falciparum malaria in Ado-Odo/Ota, Ogun State (2015–2017).Number of samples collected Number of positive casesAge group Male Female Total Male Female Total % Totalr5 68 80 148 36 33 69 46.6210–20 313 358 671 187 153 340 50.67Z20 191 203 394 69 68 137 34.77Total 572 641 1213 292 254 546 45.01Table 5Prevalence of resistance genes in Ado-Odo/Ota, Ogun State.GENES No of samples Positive samples Incidence (%)Blood Saliva Blood Saliva Blood SalivaPfcrt 71 35 34 11 47.89 31.43Pfmdr 46 30 16 8 34.78 26.67PfK13 87 18 19 8 21.84 44.44Total 204 83 69 27 33.82 32.53Table 1Incidence of falciparum malaria in Ado-Odo/Ota, Ogun State (2015).Number of samples collected Number of positive casesAGE Male Female Total Male Female Total % Incidencer5 43 56 99 24 20 44 44.4410–20 233 276 509 144 114 258 50.69Z20 126 132 258 47 33 80 31.01Total 402 464 866 215 167 382 44.11I.Ruth Diji-geske et al. / Data in Brief 19 (2018) 1398–14021400Fig. 1. Incidence of P. falciparum malaria infection in males against females.Fig. 2. Resistance genes detected in blood and saliva samples.Fig. 3. Pfcrt gene in blood and corresponding saliva samples.I.Ruth Diji-geske et al. / Data in Brief 19 (2018) 1398–1402 1401I.Ruth Diji-geske et al. / Data in Brief 19 (2018) 1398–14021402AcknowledgementCovenant University Center for Research and Discovery (CUCRID), and all staffs of the CovenantUniversity Molecular research laboratory, for their immense support in this research.Transparency document. Supporting informationTransparency data associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2018.06.002.References[1] O.M. Kenji, T.Y. Samuel, F.E. Livo, N.B. Obase, Y. Jessica, C.D. Jean, D.M. Calixt, W.T. Diane, R.N. Vivek, F.L. Rose, Detection ofPlasmodium falciparum DNA in saliva samples stored at room temperature: potential for a non-invasive saliva-baseddiagnostic test for malaria, Malar. J. 16 (2017) 434.[2] World malaria report , Geneva: World Health Organization, 〈http://www.who.int/malaria/publications/world_malaria_report_2014/en/〉, (Accessed 10 March 2018), 2014.[3] G.I. Olasehinde, O.O. Ojurongbe, E.O. Fagade, S. Ruchi, L.O. Egwari, A.A. Ajayi, O.A. Adeyeba, Detection of molecular markersof antimalarial drug resistance in Plasmodium falciparum from South-Western Nigeria, Covenant J. Phys. Life. Sci. 1 (2)(2014) 61–75.[4] C.V. Plowe, Monitoring antimalarial drug resistance: making the most of the tools at hand, J. Exp. Biol. 206 (2003)3745–3752.[5] O. Ojurongbe, T.O. Ogungbamigbe, A.F. Fagbenro-Beyioku, R. Fendel, P.G. Kremsner, J.F. Kun, Rapid detection of Pfcrt andPfmdr1 mutations in Plasmodium falciparum isolates by FRET and in vivo response to chloroquine among children fromOsogbo, Nigeria, Malar. J. 6 (2007) 41.

Epidemiological data of falciparum malaria in Ado-Odo/Ota, Southwest Ogun State, Nigeria

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Epidemiological data of falciparum malaria in Ado-Odo/Ota, Southwest Ogun State, Nigeria

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