Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: Establishment of an efficient ELISA for antigen detection in feces

Monoclonal antibodies to bovine enteric coronavirus (BEC) were produced. Additionally, polyclonal antibodies were made in rabbits and guinea pigs and extracted from the yolk of immunized hens. The antibodies were characterized by neutralization test, hemagglutination inhibition test, enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Neutralizing antibody titers of polyclonal antisera ranged from 1:1280 to 1:40 000. Only one out of 908 hybridoma colonies tested secreted antibodies with neutralizing activity. By ELISA, polyclonal sera exhibited high background reactions that could be significantly reduced by treatment with kaolin in the case of rabbit sera. Attempts to establish an ELISA for BEC antigen detection based on polyclonal sera failed due to low sensitivity and specificity. Optimal results were achieved when a mixture of two monoclonal antibodies was coated onto microplates for antigen capture, while rabbit hyperimmune serum served as detecting antibodies in an indirect assay. The combination of the two monoclonal antibodies did not increase sensitivity synergistically, but in a compensatory fashion, probably because of epitope differences between BEC field strain

Density Functional Theory of the Hubbard-Holstein Model

We present a density functional theory (DFT) for lattice models with local electron-electron (e-e) and electron-phonon (e-ph) interactions. Exchange-correlation potentials are derived via dynamical mean field theory for the infinite-dimensional Bethe lattice, and analytically for an isolated Hubbard-Holstein site. These potentials exhibit discontinuities as a function of the density, which depend on the relative strength of the e-e and e-ph interactions. By comparing to exact benchmarks, we show that the DFT formalism gives a good description of the linear conductance and real-time dynamics.Comment: 5 pages, 3 figures, supplemental material provided as pd

Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe

A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures. DNA fragments digested with restriction endonuclease BamHI were separated, transfered and immobilized on filter membranes. A Digoxigenin-labeled probe derived from EHV 1 was used for hybridization. This probe hybridized specifically to sequences of the inverted terminal repeat region which in case of Rac H include a deletion of 0.8 kb. By comparing the different migration patterns after blot hybridization it could be shown that in 65 isolates from cases of abortion the live vaccine strain Rac H was not involve

Geometry of Universal Magnification Invariants

Recent work in gravitational lensing and catastrophe theory has shown that the sum of the signed magnifications of images near folds, cusps and also higher catastrophes is zero. Here, it is discussed how Lefschetz fixed point theory can be used to interpret this result geometrically. It is shown for the generic case as well as for elliptic and hyperbolic umbilics in gravitational lensing.Comment: RevTEX4, 13 pages, submitted to J. Math. Phy

Phase transitions and the internal noise structure of nonlinear Schr\"odi nger equation solitons

We predict phase-transitions in the quantum noise characteristics of systems described by the quantum nonlinear Schr\"odinger equation, showing them to be related to the solitonic field transition at half the fundamental soliton amplitude. These phase-transitions are robust with respect to Raman noise and scattering losses. We also describe the rich internal quantum noise structure of the solitonic fields in the vicinity of the phase-transition. For optical coherent quantum solitons, this leads to the prediction that eliminating the peak side-band noise due to the electronic nonlinearity of silica fiber by spectral filtering leads to the optimal photon-number noise reduction of a fundamental soliton.Comment: 10 pages, 5 figure

Simulation of seismic events induced by CO2 injection at In Salah, Algeria

Date of Acceptance: 18/06/2015 Acknowledgments The authors would like to thank the operators of the In Salah JV and JIP, BP, Statoil and Sonatrach, for providing the data shown in this paper, and for giving permission to publish. Midland Valley Exploration are thanked for the use of their Move software for geomechanical restoration. JPV is a Natural Environment Research Council (NERC) Early Career Research Fellow (Grant NE/I021497/1) and ALS is funded by a NERC Partnership Research Grant (Grant NE/I010904).Peer reviewedPublisher PD

Serendipitous discovery of an extended X-ray jet without a radio counterpart in a high-redshift quasar

A recent Chandra observation of the nearby galaxy cluster Abell 585 has led to the discovery of an extended X-ray jet associated with the high-redshift background quasar B3 0727+409, a luminous radio source at redshift z=2.5. This is one of only few examples of high-redshift X-ray jets known to date. It has a clear extension of about 12", corresponding to a projected length of ~100 kpc, with a possible hot spot located 35" from the quasar. The archival high resolution VLA maps surprisingly reveal no extended jet emission, except for one knot about 1.4" from the quasar. The high X-ray to radio luminosity ratio for this source appears consistent with the $\propto (1+z)^{4}$ amplification expected from the inverse Compton radiative model. This serendipitous discovery may signal the existence of an entire population of similar systems with bright X-ray and faint radio jets at high redshift, a selection bias which must be accounted for when drawing any conclusions about the redshift evolution of jet properties and indeed about the cosmological evolution of supermassive black holes and active galactic nuclei in general

Triaxiality and the determination of the cubic shape parameter K3 from five observables

The absolute and the relative quadrupole shape invariants q3 and K3 provide a model independent measure of triaxiality for beta-rigid nuclei. We will show that one can obtain q3 and K3 from a small number of observables. The approximations which are made will be shown to hold within a few percent both in the rigid triaxial rotor model and the interacting boson model. The shape parameter K3 is given for an exemplary set of nuclei and is translated into effective values of the geometrical deformation parameters beta and gamma.Comment: 16 pages, 4 figure