Raman Spectroscopy for Analysis of Implants from the Dura Mater

In this paper we present results of the comparative evaluation of the structural properties of the dura mater specimens (DM), manufactured using the ”Lioplast” technology, used in the clinic in the field of atrophic processes in multiple gums recessions, using the Raman spectroscopy (RS) method. The introduced coefficients and a two-dimensional analysis that showed that the processing retains the main components and removes DNA / RNA, which increases the quality that provides access to quality materials in the treatment of multiple gum recessions. It was found that the main differences appear at wavenumbers of 835 cm−1 (tyrosine), 855 cm−1 (proline), 940 and 1167 cm−1 (GAGs, CSPGs), 1240 cm−1 (amide III), 1560 cm−1 (amide II) and 1447 cm−1 (lipids and proteins). It is shown that Raman spectroscopy can be used to evaluate implants from the dura mater. Keywords: Raman spectroscopy, dura mater, biomaterial, spectral analysis

Spectral Analysis of Structural Changes of the Heart Valves at Different Stages of Their Decellularization

Presents the results of applying the method of Raman spectroscopy for the qualitative analysis of the surfaces of the heart valve of sheep before and during their decellularization. Optical analysis showed that the implementation of decellularization valves reduces the content of glycosaminoglycans, proteins and lipids. Found that using specified optical coefficients, it is possible to control the efficiency of the process of decellularization heart valves. Keywords: Raman spectroscopy, heart valve, optical coefficient, decellularization, spectral analysis

The application of the Raman Spectroscopy Method for Evaluating Implants from the Dura Mater

The results of a comparative spectral evaluation of the component composition of the surfaces of implants from the dura mater manufactured using the Lioplast technology with the use of ultrasound and sterilization are presented. Based on the analysis, coefficients were introduced reflecting the change in the relative concentration of components that determine the quality of the implants. It is established that Raman spectroscopy can be used to assess the change in the composition of implants based on the dura mater during their manufacture. Keywords: Raman spectroscopy, coefficients, spectral features, implants, dura mater

Analysis of Synovial Fluid for Study of Diseases of Joints Using the Method of Raman Spectroscopy

This paper presents the results of experimental studies using the synovial fluid Raman spectroscopy method, which was obtained from the joint cavity during the operation. By analyzing the composition of the synovial fluid, it was found that with the development of the degenerative-dystrophic process in the synovial fluid of the affected joint, the total number of components at wave numbers: 1155 cm-1 (Hyaluronic acid (C-O, C-C)) and 1250 cm-1 (Amide III). The introduced optical coefficients allow estimating the synovial fluid in osteoarthritis and further this method of Raman spectroscopy can become a new diagnostic screening for the detection of articular pathology. Keywords: Raman spectroscopy, osteoarthrosis, synovial flui

Spectral Studies of Rat Bone Tissue in Modeling Osteoporosis and Effectiveness of Treatment By Hydroxyapatite

Presents the result of experiments on the study of the model of osteoporosis in rats using Raman spectroscopy and the effectiveness of its treatment with hydroxyapatite. Were revealed spectral differences between groups of samples (control group, group with the model of osteoporosis and a group with the model of osteoporosis after treatment with hydroxyapatite). In addition, optical coefficients were introduced to evaluate the effectiveness of treatment. Keywords: Raman spectroscopy, optical coefficients, osteoporosis, hydroxyapatite, collagen matri

Забезпечення Європейським судом з прав людини прямої дії норм Конвенції 1950 р.

Тимченко Л. Д. Забезпечення Європейським судом з прав людини прямої дії норм Конвенції 1950 р. / Л. Д. Тимченко, В. П. Кононенко // Правове забезпечення ефективного виконання рішень і застосування практики Європейського суду з прав людини : матер. 2-­ї Міжнар. наук.-­практ. конф. (Одеса, 20-­21 вересня 2013 р.) / за ред. С. В. Ківалова ; НУ «ОЮА». – Одеса : Фенікс, 2013. – С. 131-138.Стаття присвячена розгляду ставлення до діяльності суддів у про­цесі правозастосування, аналізу їх правотворчої функції. Необхід­ність ефективної дії норм конвенції 1950 р., в яких закріплено права людини і основоположні свободи, практично передбачає не тільки визнання їх природно-­правового характеру, а їх невідчужуваність й непорушність. йдеться про встановлення нових стандартів право­суддя при тлумаченні тих норм конвенції, які з огляду на специфіку юридичної природи прав людини не можуть бути сформульовані абсолютно чітко. Досить загальні положення конвенції 1950 р. в ре­зультаті інтерпретації їх Європейським судом з прав людини під час розгляду конкретних справ, забезпечують пряму дію норм, в яких закріплено права людини і основоположні свободи.В статье рассматривается отношение к деятельности судей в процес­се правоприменения, анализа их правотворческой функции. Необхо­димость эффективного действия норм Конвенции 1950 г., в которых закреплены права человека и основные свободы, практически пред­полагает не только признание их естественно­правового характера, а их неотчуждаемость и нерушимость. речь идет об установлении новых стандартов правосудия при толковании тех норм Конвенции, с учетом специфики юридической природы прав человека, которые не могут быть сформулированы абсолютно четко. Достаточно общие положения конвенции 1950 г. в результате интерпретации их евро­пейским судом по правам человека при рассмотрении конкретных дел, обеспечивают прямое действие норм, в которых закреплены права человека и основные свободы.The article is devoted attitude of judges in the enforcement analyze their law­making function. The need for effective action provisions of the Convention in 1950, which enshrined human rights and fundamental freedoms, practically involves not only the recognition of natural law, and their inalienability and inviolability. It’s about setting new standards of justice in the interpretation of the provisions of the Convention are given the specificity of the legal nature of human rights can not be defined clearly. Quite general provisions of the Convention in 1950 as a result of their interpretation by the European Court of human Rights during consideration of specific cases, providing direct effect framework in which enshrined human rights and fundamental freedoms

Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study

Background. Contemporary dental diagnosis should supplement clinical examination with instrumental diagnostic techniques. Raman spectroscopy has become widely adopted due to high spatial resolution, non-invasiveness, the lack of strict requirements and ease of sample preparation.Objectives. A qualitative assessment of enamel mineral and organic composition dynamics using Raman spectroscopy.Methods. Raman spectroscopy was used as primary research method. The trial bench consisted of a Shamrocksr-303i high-resolution digital spectrometer with built-in DV420A-OE cooled optical detector (spectral range 200–1200 nm), RPB-785 optic fibre scattering probe integrated with LuxxMaster LML-785.0RB-04 laser source with wavelength 785 nm.Software spectrum processing was performed with Wolfram Mathematica 9. Spectra were denoised with a median filter (5 points), the approximating line (fifth order polynomial) of autofluorescent component was determined in 700–2200 cm-1 range using an iterative algorithm and then subtracted to receive isolated Raman spectra.Linear discriminant analysis (LDA) of data was performed with the IBM SPSS Statistics package. Results. The assay included 28 teeth, with 14 extracted for orthodontic indications and 14 — for chronic periodontitis. Spectral dental enamel dynamics has been established in periodontal disease and after the in-office bleaching procedure. The evidence obtained can be applicable in dental practice to verify patients at risk of periodontal disease by interpreting the tooth enamel spectral properties, as well as prior to in-office bleaching.Conclusion. We demonstrate high efficiency of Raman spectroscopy for qualitative assessment of the mineral and organic enamel composition dynamics in various settings. Raman spectroscopy is confirmed effective and versatile in various aetiologies. It was successfully applied to diagnose periodontitis by changes in the organic and mineral enamel composition and evaluate enamel after in-office bleaching

Production of 180m{^{180\rm{m}}}Hf in photoproton reaction 181{^{181}}Ta(γ,p)(\gamma,p) at energy EγmaxE_{\rm{\gamma max}} = 35-95 MeV

The production of the $^{180\rm{m}}\rm{Hf}$ nuclei in the photoproton reaction ${^{181}\rm{Ta}}(\gamma,p)$ was studied at end-point bremsstrahlung energies $E_{\rm{\gamma max}}$ = 35-95 MeV. The experiment was performed at the electron linear accelerator LUE-40 NSC KIPT with the use of the $\gamma$ activation and off-line $\gamma$-ray spectroscopy. The experimental values of the bremsstrahlung flux-averaged cross-sections $\langle{\sigma(E_{\rm{\gamma max}})}\rangle_{\rm{m}}$ for the ${^{181}\rm{Ta}}(\gamma,p)^{180\rm{m}}\rm{Hf}$ reaction were determined, and at $E_{\rm{\gamma max}} > 55$ MeV obtained for the first time. The measured values, also as the literature data, are significantly exceed the theoretical flux-averaged cross-sections $\langle{\sigma(E_{\rm{\gamma max}})}\rangle_{\rm{th}}$. The $\langle{\sigma(E_{\rm{\gamma max}})}\rangle_{\rm{th}}$ values were calculated using the cross-section $\sigma(E)$ computed with the TALYS1.95 code for six different level density models. A comparative analysis of the calculated total cross-sections for the reactions ${^{181}\rm{Ta}}(\gamma,p)^{180}\rm{Hf}$ and ${^{181}\rm{Ta}}(\gamma,n)^{180}\rm{Ta}$ was performed. It was shown that the photoproton $(\gamma,p)$ to photoneutron $(\gamma,n)$ strength ratio is consistent with the estimates based on the isospin selection rules and the value from the $(e,e'p)$ experiment.Comment: 9 pages, 4 figures, 2 table

West nile fever case in neurological practice

West Nile fever is a zoonotic arbovirus mosquito- and tick-borne infection, which occurs in humans in the form of an acute febrile illness with symptoms of general intoxication, in severe cases – with the central nervous system damage. West Nile virus belongs to the Flaviviridae family.After the start of Russians’ mass tourism to tropical and subtropical regions, number of cases of the disease in Russia has increased, especially in the south, where the virus is more viable. The infection mainly affects birds but also people and many mammals (bats, cats, dogs, skunks, squirrels, rabbits, etc.), which become infected after the bite of a mosquito of the genus Culex (Culex pipiens) carrying the disease, ixodid or argas mite. A clinical case of hemorrhagic stroke caused by West Nile fever is presented in the article