Prevalence and cause of gestational diabetes mellitus in a tertiary care center in Kolar district: a population based study

Background: Gestational diabetes mellitus (GDM) is carbohydrate intolerance at the onset of pregnancy which induces pathological short term or long term outcomes for both mother and baby. The aim of the present study was to know the prevalence of GDM in pregnant women who were attending the antenatal care (ANC) center at a tertiary care hospital in Kolar, Karnataka, India.Methods: This prospective study was conducted in Department of Obstetrics and Gynecology, Sri Devaraj Urs Medical College, a constituent of Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India. The duration of the study was two months. In this study, 108 pregnant women above 24 weeks of gestation were screened for GDM by oral glucose tolerance test. Fasting 2 milli liter blood was collected and were given 75 grams of glucose in 200 milli liters of water and asked to drink within 5 minutes. Again 2 milli liters venous blood was collected after 1 hour and 2 hours from all participants. Plasma sample was used for the estimation of glucose by glucose oxidase and peroxidase (GOD-POD) method.Results: Out of 108, 12 women (11.1%) were diagnosed with GDM. The prevalence rate was higher in the age group of 26-30 years (41.6%).  Among 12 diabetic women, five (47.2%) exercised regularly and seven (58.3%) did not doing exercise. Out of 12 GDM subjects, eight of them had family history of diabetes in first degree relatives; among which one was hypertensive and five were suffering from thyroid problems.Conclusions: In the present study, the prevalence of GDM was found to be 11.1%. Prevalence of GDM might be influenced by increasing age, pre pregnancy weight, family history of diabetes, past history of pregnancy complications, status of literacy and exercise

Block-Chain-Based Vaccine Volunteer Records Secure Storage and Service Structure

Accurate and complete vaccine volunteer’s data are one valuable asset for clinical research institutions. Privacy protection and the safe storage of vaccine volunteer’s data are vital concerns during clinical trial services. The advent of block-chain technology fetches an innovative idea to solve this problem. As a hash chain with the features of decentralization, authentication, and resistibility, blockchain-based technology can be used to safely store vaccine volunteer clinical trial data. In this paper, we proposed a safe storage method to control volunteer personal /clinical trial data based on blockchain with storing on cloud. Also, a service structure for sharing data of volunteer’s vaccine clinical trials is defined. Further, volunteer blockchain features are defined and examined. The projected storage and distribution method is independent of any third person and no single person has the complete influence to disturb the processing.

In vitro haemolytic, antioxidant and antibacterial (ESBLs and MRSA) activity of Datura metel L. flower and leaf extracts

500-506Prevalence of antibiotic resistant bacteria is an issue of concern as it affects the efficiency of antibiotics significantly. It makes the drug manufacturers continuously search for newer molecules that are safe and effective. To meet this challenge, researchers look towards natural resources, particularly plants, for possible new molecules that can be potentially tapped for medicinal purposes. In this context, here, we investigated Datura metel, commonly called the Angel’s trumphet as it is known for medicinal properties. We studied the hot water and methanol extracts of its flowers and leaves for antibacterial, antioxidant and antihemolytic activities. We used disc diffusion, phospho-molybdate and spectrophotometric methods. The bacteria tested were Methicillin resistant Staphylococcus aureus (MRSA) and β lactamase (ESBL) producing E. coli. The zone of inhibition (ZoI), as a measure of bacterial growth inhibition, was checked and found to range from 1.2-17.5 mm at the tested concentrations; ESBL producing E. coli was more subtle than MRSA. Total antioxidant capacity measured at 695 nm showed an average score of 17.4 μg/mL, displaying better activity of methanol extract. Antihemolytic property was tested on 0.5% RBC and percentage hemolysis was measured at optical density of 540 nm. Both the extracts showed considerably similar activity, though hot water lowered the hemolytic activity. Over all, the results suggest that D.metel could be exploited for its potential to inhibit drugresistant bacteria; free radical scavenging activity; and anti-hemolytic properties

Metallurgical investigation of cracked Al–5.5Zn–2.5Mg–1.5Cu aluminium alloy valve

AbstractThe high strength aluminium alloy Al–5.5Zn–2.5Mg–1.5Cu (AA7075) is being widely used in realisation of aerospace components. A component ‘fill and vent valve’ used in liquid propulsion system was fabricated from AA 7075 forgings in T7352 temper condition, and subsequently undergone various functional tests, four years back. Recently, during dye penetrant test after proof pressure test at 525bar, a valve indicated presence of a crack. Detailed metallurgical investigation indicated that failure was caused by stress corrosion cracking

A SECRET-KEY OF FLEXIBLE CHOICE ON CONFIDENTIAL OUTSIDERS FILES

Expansion for data outsourcing may be used becoming an important understanding for a lot of programs. Inside the recent occasions, the efforts that have been made earlier mainly spotlight on minimization of communication needs. Inside the recent cryptography techniques, the essential concern is regarding leveraging of confidentiality of knowledge to deal with cryptographic functions numerous occasions. We produce a study making of understanding key more commanding so it permits knowledge of several cipher-texts, missing of the size increase. We commence an excellent public-key file encryption known to as key-aggregate cryptosystem. Cryptographic techniques of key assignment decreases spending in storing additionally to controlling of secret keys for wide-different cryptographic use. We study a novel cryptosystems of public-key that leave constant size cipher-texts for competent delegation of understanding legal rights for possible cipher-texts. Our strategy is flexible when compared to hierarchical key assignment that saves spaces when the entire key-holders distribute an connected number of legal rights

DEFINITE OUTLAY OPTIMALITY BY SERVING VOLATILE REQUESTS

Several projects were emerged inside the yesteryear couple of years that explore migration of services into cloud platform. More novel programs were created on cloud platform while numerous traditional programs are in addition considering cloud-ward move including programs of content distribution programs. Two important tasks are concerned for just about any go to transfer contents towards cloud storage, also to allocate web service load towards cloud-based web services. Inside our work we design dynamic control formula to place contents and dispatch demands in the hybrid cloud system spanning geo-distributed data centres that reduces general operational expenditure ultimately, prone to the limitations and services information response time

In vitro haemolytic, antioxidant and antibacterial (ESBLs and MRSA) activity of Datura metel L. flower and leaf extracts

Prevalence of antibiotic resistant bacteria is an issue of concern as it affects the efficiency of antibiotics significantly. It makes the drug manufacturers continuously search for newer molecules that are safe and effective. To meet this challenge, researchers look towards natural resources, particularly plants, for possible new molecules that can be potentially tapped for medicinal purposes. In this context, here, we investigated Datura metel, commonly called the Angel’s trumphet as it is known for medicinal properties. We studied the hot water and methanol extracts of its flowers and leaves for antibacterial, antioxidant and antihemolytic activities. We used disc diffusion, phospho-molybdate and spectrophotometric methods. The bacteria tested were Methicillin resistant Staphylococcus aureus (MRSA) and β lactamase (ESBL) producing E. coli. The zone of inhibition (ZoI), as a measure of bacterial growth inhibition, was checked and found to range from 1.2-17.5 mm at the tested concentrations; ESBL producing E. coli was more subtle than MRSA. Total antioxidant capacity measured at 695 nm showed an average score of 17.4 μg/mL, displaying better activity of methanol extract. Antihemolytic property was tested on 0.5% RBC and percentage hemolysis was measured at optical density of 540 nm. Both the extracts showed considerably similar activity, though hot water lowered the hemolytic activity. Over all, the results suggest that D. metel could be exploited for its potential to inhibit drug resistant bacteria; free radical scavenging activity; and anti-hemolytic properties

Evaluation of QTLs for Shoot Fly (Atherigona soccata) Resistance Component Traits of Seedling Leaf Blade Glossiness and Trichome Density on Sorghum (Sorghum bicolor) Chromosome SBI-10L

Shoot fly is a major insect pest of sorghum damaging early crop growth, establishment and productivity. Host plant resistance is an efficient approach to minimize yield losses due to shoot fly infestation. Seedling leaf blade glossiness and trichome density are morphological traits associated with shoot fly resistance. Our objective was to identify and evaluate QTLs for glossiness and trichome density using- i) 1894 F2s, ii) a sub-set of 369 F2-recombinants, and iii) their derived 369 F2:3 progenies, from a cross involving introgression lines RSG04008-6 (susceptible) × J2614-11 (resistant). The QTLs were mapped to a 37–72 centimorgan (cM) or 5–15 Mb interval on the long arm of sorghum chromosome 10 (SBI-10L) with flanking markers Xgap001 and Xtxp141. One QTL each for glossiness (QGls10) and trichome density (QTd10) were mapped in marker interval Xgap001-Xnhsbm1044 and Xisep0630-Xtxp141, confirming their loose linkage, for which phenotypic variation accounted for ranged from 2.29 to 11.37 % and LOD values ranged from 2.03 to 24.13, respectively. Average physical map positions for glossiness and trichome density QTLs on SBI-10 from earlier studies were 4 and 2 Mb, which in the present study were reduced to 2 Mb and 800 kb, respectively. Candidate genes Glossy15 (Sb10g025053) and ethylene zinc finger protein (Sb10g027550) falling in support intervals for glossiness and trichome density QTLs, respectively, are discussed. Also we identified a sub-set of recombinant population that will facilitate further fine mapping of the leaf blade glossiness and trichome density QTLs on SBI-10

Fine mapping of stay-green QTLs on sorghum chromosome SBI-10L ‒ An approach from genome to phenome

Sorghum is the fifth most important C4 cereal crop grown globally in arid and semi-arid climatic conditions. Drought is the major cause for loss of productivity worldwide. Delayed senescence of plants leads to adaptation to drought stress conditions by staying-green and giving high yields. In order to identify and dissect the stay-green genomic regions we have developed a high resolution fine mapping population from introgression line cross RSG04008-6 (stay-green) × J2614-11 (shoot fly resistant). Nearly 1894 F2 genotypes were screened with 8 SSR in order to identify double recombinants for both the parents in the sorghum chromosome-10 long arm (SBI-10L). The selected F2:4 recombinants were GBSed to increase the marker density between flanking markers Xgap001- Xtxp141 for stay-green QTLs on SBI-10L and a high resolution linkage map was developed using GBS SNPs. Out of 182 only 152 recombinants were replicated thrice in field for staygreen screening for two seasons (Summer 2013 and 2014). Fine mapping of the per cent Green Leaf Area (%GLA) traits identified 33 QTLs and 19QTLs were clustered into 7groups where 8 genes were identified. These were AP2/ERF transcription factor family (Sb10g025053), Ankyrin-repeat protein (Sb10g025310), WD40 repeat protein (Sb10g025320), NBSLRR Protein (Sb10g025283), Calcium dependant protein kinase (Sb10g030150), LEA2 protein (Sb10g029570), a putative uncharacterized protein (Sb10g024920) and senescence associated protein (Sb10g030520). With this study SBI-10L staygreen genomic regions were delimited from 15Mb to 8 genes co-localized with GWAS MTAs. Further cloning and expression level studies of the identified candidate genes will improve the development of drought tolerant genotypes